Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

The Effects of Dex and PDGF-BB on Bony Healing of Calvarial Defect in Rats

골재생 과정에서 혈소판유래성장인자-BB와 덱사메타존의 병용 효과

  • Lee, Jae-Mok (Department of Periodontology, College of Dentistry, Kyungpook National University) ;
  • Park, Jin-Woo (Department of Periodontology, College of Dentistry, Kyungpook National University) ;
  • Suh, Jo-Young (Department of Periodontology, College of Dentistry, Kyungpook National University)
  • 이재목 (경북대학교 치과대학 치주과학교실) ;
  • 박진우 (경북대학교 치과대학 치주과학교실) ;
  • 서조영 (경북대학교 치과대학 치주과학교실)
  • Published : 2003.12.31
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Abstract

Bone remodeling results from the combined process of bone resorption and new bone formation which is regulated in part by some of Dexamethasone related proliferation & mineralization of cultured bone cell and polypeptide growth factors such as platelet derived growth factor(PDGF), which has been known to be an important local regulator of bone cell activity and participate in normal bone remodeling. To evaluate the effects of Dex and PDGF on bony healing of calvarial defect in rats, 10 ng/ml PDGF were applied on P group and 10 ng/ml PDGF and $10^7$ M Dex were applied PD group. 4 rats in each group were sacrificed at 7, 14. 21 days after operation respectively, and the tissue blocks were prepared for light microscope with H-E for evaluation of overall healing, with TRAP(tartrate resistant acid phosphatase) for evaluation of osteoclastic activity and with immunohistochemical staining for macrophages. The results were as follows : 1. In all group, healing aspects were progressed from 7 days to 21 days in soft and bony tissue, but complete repair were not observed in bony defect 2. PDGF and control group were showed similar bony healing aspect , but bony healing in combination of PDGF-BB and Dex were observed slower aspect compared to PDGF and control group from early healing times. 3. There were no significant difference on activities of osteoclast and macrophages in bony healing between control and experimental group In conclusion, PDGF were not influenced on bony healing of defect and combination of PDGF-BB and Dex were showed slower healing through early healing times. it was considered that Dex compared to PDGF did influenced on early hone formation factors in healing period

Keywords

References

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