Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effects of enamel matrix derivative and titanium on the proliferation and differentiation of osteoblasts

법랑기질유도체를 도포한 타이태늄 표면에서 조골세포의 증식 및 분화

  • Park, Sang-Hyun (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Lee, In-Kyeong (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Yang, Seung-Min (Department of Dentistry, College of Medicine, Sung Kyun Kwan University) ;
  • Shin, Seung-Yun (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Lee, Yong-Moo (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Ku, Young (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Rhyu, In-Chul (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Chung, Chong-Pyoung (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Han, Soo-Boo (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Choi, Sang-Mook (Department of Periodontology, College of Dentistry, Seoul National University)
  • 박상현 (서울대학교 치과대학 치주과학교실) ;
  • 이인경 (서울대학교 치과대학 치주과학교실) ;
  • 양승민 (성균관대학교 의과대학 치과학교실) ;
  • 신승윤 (서울대학교 치과대학 치주과학교실) ;
  • 이용무 (서울대학교 치과대학 치주과학교실) ;
  • 구영 (서울대학교 치과대학 치주과학교실) ;
  • 류인철 (서울대학교 치과대학 치주과학교실) ;
  • 정종평 (서울대학교 치과대학 치주과학교실) ;
  • 한수부 (서울대학교 치과대학 치주과학교실) ;
  • 최상묵 (서울대학교 치과대학 치주과학교실)
  • Published : 2003.09.30
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Abstract

Among objectives of periodontal therapy. the principal one is the morphological and functional reconstruction of lost periodontal supporting tissues. This includes de novo formation of connective tissue attachment and the regrowth of alveolar bone. The use of enamel matrix derivative(EMD) may be a suitable means of regeneration new periodontal attachment in the infrabony defects. Implant used to replace lost tooth but, implantitis occurred after installation. The purpose of this study was to investigate the effects of EMD on differentiation and growth of osteoblast in titanium disc. Twentyfive millimeter diameter and 1mm thick Ti disc which was coated 25, 50, 100, 200${\mu}g$/ml of EMD(Emdogain(R)) used as experimental group, 25, 50, 100, 200ng/d of rhBMP-2 as positive control group, and no coat as negative control group. A human osteosarcoma cell line Saos-2 was cultured in Ti disc and cell proliferation and Alkaline phosphatase (ALP) activity were measured at 1 and 6 days. PCR was performed at 2 and 8 hours. Semi-quantitative RT-PCR for mRNA expressions of various osteoblastic differentiation markers -type I collagen, ALP, osteopontin, and bone sialoprotein - were performed at appropriate concentrations based upon the results of MTT and ALP assay. Cultured cell-disc complexes were prepared for scanning electron microscopy (SEM) at 2 hour. Data were analyzed using Mann-Whitney and repeated- measures 1-way analysis of variance(SPSS software version 10,SPSS. Chicago. IL). After culture, there was more osteoblast in EMD100${\mu}g$/ml than in EMD50, 200${\mu}g$/ml on day 6. There was significant difference in experimental and positive control group compared control group, as times go by(1 and 6 days). Alkaline phosphatase activity was different significantly in EMD100, 200${\mu}g$/ml and BMP100, 200${\mu}g$/ml on day 6. The results of reverse transcriptase-polymerase chain reaction (RT-PCR) showed that expression of mRNA for ALPase, collagen type I, osteopontin. hone sialoprotein and BMP-2 was detected at 2 hour and 8 hour in EMI 200${\mu}g$/ml subgroup and BMP100ng/ml subgroup. The results of this study suggest that application of enamel matrix derivative on osteoblast attached to titanium surface facilitate the expression of bone specific protein and the differentiation and growth of osteoblast.

Keywords

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