Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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GCF concentration and minimal inhibitory concentration of cefuroxime axetil against periodontopathogens

Cefuroxime axetil의 치주병원성 세균에 대한 최소억제농도와 치은열구액 농도에 관한 실험적 연구

  • Jang, Hyun-Seon (Department of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Kim, Jae-Duk (Department of Periodontology, College of Dentistry, Chosun University) ;
  • Kook, Joong-Ki (Department of Oral Biochemistry, College of Dentistry, Chosun University, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Kim, Hwa-Sook (Department of Oral Biochemistry, College of Dentistry, Chosun University, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • You, So-Young (Department of Oral Biochemistry, College of Dentistry, Chosun University, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Kim, Mi-Kwang (Department of Oral Biochemistry, College of Dentistry, Chosun University, Oral Biology Research Institute, College of Dentistry, Chosun University) ;
  • Kim, Byung-Ock (Department of Periodontology, College of Dentistry, Chosun University, Oral Biology Research Institute, College of Dentistry, Chosun University)
  • 장현선 (조선대학교 치과대학 치주과학교실, 조선대학교 구강생물학연구소) ;
  • 김재덕 (조선대학교 치과대학 치주과학교실) ;
  • 국중기 (조선대학교 치과대학 구강생화학교실, 조선대학교 구강생물학연구소) ;
  • 김화숙 (조선대학교 치과대학 구강생화학교실, 조선대학교 구강생물학연구소) ;
  • 유소영 (조선대학교 치과대학 구강생화학교실, 조선대학교 구강생물학연구소) ;
  • 김미광 (조선대학교 치과대학 구강생화학교실, 조선대학교 구강생물학연구소) ;
  • 김병옥 (조선대학교 치과대학 치주과학교실, 조선대학교 구강생물학연구소)
  • Published : 2003.09.30
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Abstract

The purpose of this study was to determine the minimal inhibitory concentration (MIC) of cefuroxime axetil,　semisynthetic cefalosporin, for some putative periodotopathogens; F. nucleatum, A. actinomycetemcomitans P. intermedia and P. gingivalis. To investigate the efficacy of cefuroxime axetil, several antibiotics, amoxicillin, metronidazole, and ciprofoxacine, were used as control. The MIC was measured by Murray' s method. The MIC of cefuroxime axetil against some putative microbes, as a single use regimen, was relatively high in comparison with that of the other antibiotics used in this study. The MIC of cefuroxime axetil/metronidazole against some putative microbes, as a simultaneous regimen, was similar to that of the other antibiotics used in this study. The manimal level of cefuroxime concentration in gingival fluid was 9${\mu}$/ml at 36hr after the first dose. In conclusion, within the limited experiment, metronidazole/ cefuroxime axetil therapy of periodontitis may provide a therapeutic benefits in reducing the periodontopathogens.

Keywords

References

  1. van Winkelhoff A, Rams TE, Slots J. Systemic antibiotic therapy in periodontitis. Periodontol 2000 1996;10:45-78 https://doi.org/10.1111/j.1600-0757.1996.tb00068.x
  2. Socransky SS, Haffajee AD. Evidence of bacterial etiology: Historical perspective. Periodontol 2000 1994;2:1-7
  3. Greenstein G, Lamster I. Changing Periodontal Paradigms: Therapeutic Implications. Int J Periodontics Restorative Dent 2001;2:11-31
  4. Haffajee AD, Socransky SS. Microbial etiological agents of destructive periodontal diseases. Periodontol 2000 1994;5:78-111 https://doi.org/10.1111/j.1600-0757.1994.tb00020.x
  5. Position Paper. Systemic Antibiotics in Periodontics. J Periodontol 1996;67:831-838
  6. Murray PR, Jorgensen JH. Quantitative susceptibility test methods in major united states medical center. Antimicrobial agent and chemotherapy 1981;20:66-70 https://doi.org/10.1128/AAC.20.1.66
  7. Gordon JM, Walker CB, Goodson JM, Socransky SS. Sensitivity assay for measuring tetracycline levels in gingival crevice fluid. Antimicrob Agents Chemother 1980;17:193-198 https://doi.org/10.1128/AAC.17.2.193
  8. Ciancio SG. Perio-chernotherapeutics: New directions in Periodontal Disease Maanagement. American Academy of Periodontology 1994:315-335
  9. Golub LM, Ryan ME, William RC. Modulation of the host response in the treatment of periodontitis. Dentistry today 1998;17:123-127
  10. Lindhe J. Clinical periodontology and Implant Dentistry, 3rd ed. UNKSGAARD, 2000:488-507
  11. Genco RJ. Systemic antimicrobials in the management of periodontal diseases in Periodontal Disease Management. American Academy of Periodontology. 1994:237-252
  12. Griffiths GK, Wight LJ, Gudgeon AC, Kelsey M. Efficacy and tolerability of cefuroxime axetil in patients with upper respiratory tract infections. Curr. Med, Res. Opi. 1987;10(8):131-137
  13. Bragd L, Wikstrom M, Slots J. Clinical and microbiological study of refractory adult periodontitis. J Dent Res 1985;64:234
  14. Korman KS, Newman MG, Moore DJ, Singer RE. The influence of supragingival plaque control on clinical and microbial outcomes following the use of antibiotics for the treatement of periodontitis. J Periodontol 1994;65:848-854 https://doi.org/10.1902/jop.1994.65.9.848
  15. Christersson LA, Zambon JJ, Wikesjo UME, et al. The effects of systemic tetracycline alone on localized juvenile periodontitis. J Dent Res 1986;65:805
  16. Mandell RL, Socransky SS. Microbiological and clinical effects of surgery plus doxycycline on juvenile periodontitis. J Periodontol 1988;59:373-379 https://doi.org/10.1902/jop.1988.59.6.373
  17. Slots J, Feik D, Rams TE. In vitro antimicrobial sensitivity of enteric rods and pseudomonads from advanced ault periodontitis. Oral Microbiol Immunol 1990; 5:298-301 https://doi.org/10.1111/j.1399-302X.1990.tb00428.x
  18. Rams TE, Feik D, Slots J. Ciprofloxacin/metromi-dazole treatment of recurrent adult periodontitis. J Dent Res 1992;71:319
  19. Slots J, van Winkelhoff AJ. Antimicrobial therapy in periodontics. J Cal Dent Assoc 1993;21:51-56
  20. Loesche WJ, Syed SA, Morrison EC, et al, Metronidazole in periodontitis: I. Clinical and bacteriological results after 15 to 30 weeks. J Periodontol 1984; 55:325-335 https://doi.org/10.1902/jop.1984.55.6.325
  21. Loesche WJ, Giordano JR, Hujoel P, Schwarcz J, Smith BA. Metronidazole in periodontitis: III. Reduced need for surgery. J Clin Periodontol 1992;19:103-112 https://doi.org/10.1111/j.1600-051X.1992.tb00448.x
  22. van Winkelhoff AJ, Rodenburg JP, Goene RJ, et al, Metronidazol plus amoxycillin in the treatment of Actinobacillus actinomycetemcomitans associated periodontitis. J Clin Periodontol 1989;16:128-131 https://doi.org/10.1111/j.1600-051X.1989.tb01626.x
  23. van Winkelhoff AJ, Tijhof CJ, de Graaff J. Microbiological and clinical results of metronidazol plus amoxicillin therapy in Actinobacillus actinomycetemcomitans associated periodontitis. J Periodontol 1992;63:52-57 https://doi.org/10.1902/jop.1992.63.1.52
  24. Gordon JM, Walker CB, Murphy JC, Goodson JM, Socransky SS. Tetracycline: Levels achievable in gingival crevice fluid and in vitro effect on subgingival organisms: Partl, Concentrations in crevicular fluid after repeated doses. J periodontol 1981;52(10):609-612 https://doi.org/10.1902/jop.1981.52.10.609
  25. Tonetti M, Cugini MA, Goodson JM. Zero-order delivery with periodontal placement of tetracycline-loaded ethylene vinyl acetate fibers. J Periodont Res 1990;25:243-249 https://doi.org/10.1111/j.1600-0765.1990.tb00911.x
  26. Friesen LR, Williams KB, Krause LS, Killoy WJ. Controlled local delivery of tetracycline with polymer strips in the treatment of periodontitis. J Periodontol 2002;73:13-19 https://doi.org/10.1902/jop.2002.73.1.13
  27. Rams TE, Slots J. Antibiotics in Periodontal Therapy: An update. Compendium 1992;13:1130, 1132,1134