Abstract
The optimum conditions for the residue analysis of hydroxyanilide fungicide fenhexamid on cucumber, strawberry and grape were investigated by gas chromatography equipped with electron capture detector (GC-ECD) and the residual amount was determined by sprayed days before harvest. Each samples were extracted with acetone, filtered and concentrated to 50 mL. The concentrated extracts were transferred to dichloromethane and then thoroughly concentrated. The concentrated phase was loaded on the filtration column stuffed with silica gel and purified with acetone:hexane (5:95, 15:85, v/v) mixed solvent. The regression equation and linearity of the standard calibration curves between 0.05~2.00 ng were as follows : cucumber; Y=312.40X+10.26, $R^2=0.9996$, strawberry; Y=313.33X+5.54, $R^2=0.9998$, grape; Y=253.27X-2.23, $R^2=0.9994$. From the standard additional experiments with 0.10 mg/L and 0.40 mg/L, the average recoveries of cucumber, strawberry and grape were 94.8%, 88.1% and 93.7%, respectively and the detection limits were all the same as 0.01 mg/L. Residual amounts in crops were ranged from 0.01 to 0.58 mg/L.
Hydroxyanilide계 살균제 fenhexamid의 작물 (오이, 딸기, 포도) 중 잔류량을 전자포획검출기가 장착된 기체크로마토그래피를 이용하여 최적 분석조건을 설정하고, 처리시기별 잔류량을 구하였다. 각 시료를 blender로 마쇄 후 acetone으로 1차 추출 한 후 dichloromethane으로 2차 추출하였으며, silica gel이 충전된 plastic 컬럼에서 acetone:hexane (5:95, 15:85, v/v) 혼합액으로 분리하였다. 표준 검량선의 회귀방정식은 0.05~0.20 ng에서 오이 Y=312.40X+10.26, 딸기 Y=313.33X+5.54, 포도 Y=253.27X-2.23 이었으며, 각각 $R^2=0.9996$, $R^2=0.9998$, $R^2=0.9994$의 직선성을 나타내었다. 0.10 mg/L 과 0.40 mg/L의 표준시료 처리시 평균 회수율은 오이, 딸기, 포도 각각 94.8%, 88.1%, 93.7% 이었으며, 순수표준품의 검출한계는 0.01 mg/L이었다. 각 작물체의 잔류량은 0.01~0.58 mg/L 범위이었다.
