Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Effect of Porous Membrane on Culture Properties of Blood-Brain Barrier Endothelial Cell

다공성막이 혈액뇌관문 내피세포의 배양에 끼치는 영향

  • Lee, Keum-Jeong (Department of Anatomy, College of Medicine, Chung-Ang University) ;
  • Cho, Hye-Jin (Department of Anatomy, College of Medicine, Chung-Ang University) ;
  • Choi, Hyung-Taek (Department of Anatomy, College of Medicine, Chung-Ang University) ;
  • Na, O-Soon (Department of Anatomy, College of Medicine, Chung-Ang University) ;
  • Kim, Kyung-Yong (Department of Anatomy, College of Medicine, Chung-Ang University)
  • 이금정 (중앙대학교 의과대학 해부학교실) ;
  • 조혜진 (중앙대학교 의과대학 해부학교실) ;
  • 최형택 (중앙대학교 의과대학 해부학교실) ;
  • 나오순 (중앙대학교 의과대학 해부학교실) ;
  • 김경용 (중앙대학교 의과대학 해부학교실)
  • Published : 2003.12.01
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Abstract

The growth patterns of primary culture of bovine brain microvessel endothelial cells (BBMECs) were studied using electron microscopy when grown on $3.0{\mu}m$ and $0.4{\mu}m$ pore Transwell. The capillary fragments and isolated endothelial cells grew on collagen coated culture plate and Transwell membrane. The BBMECs grew only on the upper surface of membrane of $0.4{\mu}m$. But BBMECs on $3.0{\mu}m$ pore membrane migrated through the pore and grew on the opposite side of the membrane. In summary, BBMECs isolated by enzyme digestion could migrate through $3.0{\mu}m$ pore membrane but not through $0.4{\mu}m$ pore membrane. So $0.4{\mu}m$ pore membrane instead of $3{\mu}m$ pore membrane should be used for drug transport experiment or transendothelial electrical resistance measurement.

소 뇌 미세혈관에서 분리한 내피세포(BBMECs)를 직경 $3.0{\mu}m$및 $0.4{\mu}m$인 구멍을 가지는 다공성막(Transwell)에서 일차배양하였을 때의 특징을 전자현미경을 사용하여 살펴보았다. 분리된 모세혈관의 작은 조각과 분리된 내피세포들은 콜라겐으로 도포한 배양기구의 표면에 고착되어 성장하였다. BBMECs들은 직경 $0.4{\mu}m$인 다공성막의 위쪽 구획에서만 성장하였으나 직경 $3.0{\mu}m$인 구멍을 가진 막에서는 세포들이 구멍을 통해 막의 반대쪽으로 이주하여 다공성막의 아래쪽 구획에서도 성장하여 세포단층을 형성하였다. 이상의 결과로 효소 처리에 의해 분리한 BBMECs는 직경 $0.3{\mu}m$의 다공성막을 통과하나 직경 $0.4{\mu}m$의 다공성막을 통과할 수 없음을 알 수 있었으며, 약물이동도를 관찰하는 실험이나 전기저항을 측정을 목적으로 하는 실험에서는 $3.0{\mu}m$의 다공성막을 사용하는 대신 $0.4{\mu}m$ 크기의 다공성막을 사용해야 한다는 것을 알 수 있었다.

Keywords

References

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