Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Study on the Effect of Vitamin E on Cultured Hippocampal Neurons Damaged by Hydrogen Peroxide

과산화수소로 손상된 배양 해마신경세포에 대한 Vitamin E의 영향에 관한 연구

  • Lee Jung Hun (School of Medicine Wonkang University) ;
  • Lee Joung Hwa (School of Medicine Wonkang University) ;
  • Cho Nam Su (Department Emergency, School of Medicine Chosun University)
  • Published : 2003.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

To clerify the cytotoxicity of reactive oxygen species in cultured hippocampal neurons of neonatal mouse, toxic effect was measured by MTT assay after cultured cells were incubated for 3 hours in the media containing 1～40 μM concentrations of H₂O₂. In addition, the protective effect of vitamin E was determined in these cultrures. Cell viability was significantly decreased in a dose-dependent manner after exposure of 10 μM H₂O₂ to cultured mouse hippocampal neurons for 5 hours. In the protective effect of vitamin E, vitamin E prevented the H₂O₂-induced cytotoxicity in these cultures. From these results, it suggests that H₂O₂ has toxic effect in cultured mouse hippocampal neurons and vitamin E has protective effect on the cytotoxicity induced by H₂O₂.

Keywords

References

  1. Diabetes v.40 Role of oxidative stress in development of complication in diabetes. Baynes JW https://doi.org/10.2337/diabetes.40.4.405
  2. Nature (London) v.362 Mutation in Cu /Zn superoxide dismutase gene are associated wigh familial amyotrophic lateral sclerosis. Rosen D;Siddique T;Patterson D;Figlewiez D, Sapp P, Hentati A;Donaldson D;Goto J;0 Regan J;Deng H;Rahmani Z;Krrizus A(et al) https://doi.org/10.1038/362059a0
  3. FASEB J v.8 Oxidative DNA damage correlates with oxygen consumption in humans. Loft S.;Astrup A.;Buemann B.;Poulsen H.E. https://doi.org/10.1096/fasebj.8.8.8181672
  4. J Neurosci Res v.29 Oligodendroglial cell death induced by oxygen radicals and its protection by catalase. Kim YS;Kim SU https://doi.org/10.1002/jnr.490290111
  5. J Appl Physiol v.68 Antioxidants and antioxidant enzymes protect against pulmonary oxygen toxicity in the rabbit. Jacobson J. M.;Michael J. R.;Jafri M. H.;Gurtner G. H. https://doi.org/10.1063/1.346726
  6. J Biolo Chern. v.264 Hyperglycemic can cause membrane lipidpero¬xidation and osmic fragility in human red blood cells. Jain SK
  7. Nature v.353 Long-term potentiation in the hippocampus is blocked by tyrosine kinase inhibitors. O'Dell T. J.;Kandle E. R.;Grant S. G. N. https://doi.org/10.1038/353558a0
  8. J Neurochem v.49 Effects of methyl prednisolone and the combination of alpha-tocopherol and selenium on arachidonic acid metabolism and lipid peroxidatiom in traumatized spinal cord tissue. Saunders R.D.;Dugan L.L.;Demediuk P.;Means E.D.;Harrocks L.A.;Anderson D.K. https://doi.org/10.1111/j.1471-4159.1987.tb03388.x
  9. Neurotoxicol v.17 Methylmercury¬induced neurotoxicity in cerebral neuron culture is blocked by antioxidants and NMDA receptor antagonists. Park ST;Lim KT;Chung YT;Kim SU
  10. Kor J Gerontol v.8 no.3 Effect of Vitamin E on Cultured Mouse Cerebral Neurons Damaged by Oxidative stress. Cho C. G.;Kim H. M.;Park S. T.
  11. The Maillard reaction in food processing, human nutrition and physiology Antioxidative effect of Maillard reaction products in vivo. Chuyen N. V.;Utsunomiya N.;Hodaka A.;Kato H.; PA Pinot(Ed.)
  12. FASEB J v.9 Oxidative processes and antioxidative defense mechanism in the aging brain. Reiter RJ https://doi.org/10.1096/fasebj.9.7.7737461
  13. Agric Biol Chem v.53 Scavenging of active oxygens by melanoid in. Hayase F.;Hirashima S.;Okamoto G.;Kato H. https://doi.org/10.1271/bbb1961.53.3383
  14. Diabetes Care v.14 Vitamin E reduction of protein glycosylation in diabetes. Ceriello A;Giugliano D;Quatraro A;Donzella C;Dipalo G;Lefebvre PJ https://doi.org/10.2337/diacare.14.1.68
  15. J Neurosci Res v.36 Glutamate neurotoxicity in mesencephalic dopaminergic neurons in culture. Kikuchi S.;Kim S. U. https://doi.org/10.1002/jnr.490360508
  16. J lmmunol Methods v.65 Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Mosmann T https://doi.org/10.1016/0022-1759(83)90303-4