Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of ethyl acetate layer of Prunellae Spica on the induction of apoptosis in U937 cells

하고초 ethyl acetate분획의 U937세포에 대한 세포고사 유도효과

  • Lee Eun Ok (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University) ;
  • Kim Sung Hoon (Department of Oncology, Graduate School of East-West Medical Science, Kyunghee University)
  • 이은옥 (경희대학교 동서의학대학원 종양학실험실) ;
  • 김성훈 (경희대학교 동서의학대학원 종양학실험실)
  • Published : 2003.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Prunellae Spica is a flower petal of Prunella vulgaris var. lilacina used for treatment of lymphoma, breast cancer, hepatitis and pathological fluid related diseases in oriental medicine. We tried to evaluate the mechanism of Prunellae Spica in the treatment of cancer. The ethyl acetate layer of Prunellae Spica showed a good cytotoxicity on U937 cells with IC50 of 8 ug/ml. It induced apoptosis in U937 dose-dependently by cell cycle analysis following PI staining. We also confirmed it induced DNA fragmentation in U937 cells from the concentration of 10 ug/ml. From western blot assay we observed the ethyl acetate layer of Prunellae Spica downregulated procaspase-3 and cleaved PARP in a dose dependent manner, whereas it didn't affect bax and bcl-2. Taken together, these results indicate the ethyl acetate layer of Prunellae Spica can induce apoptosis in U937 cells suggesting it can be potently applied to cancer.

Keywords

References

  1. 대한 약전 제 7개정 해설 한국 약학대학 협의회 약전분과회
  2. Korean Folk Medicine v.121 Sun Ju Lee
  3. 동의병리학회지 용담사간탕 및 조구등, 하고초, 차전자 가미방이 고혈압에 미치는 영향 김의태;최승훈;안규전;문준전
  4. Life Sci. v.67 no.23 A comparision of human immunodeficiency virustype-a protease inhibition activities by the aqueous and methanol extracts of chinrse medicinal herbs. Lam, TL.;Lam ML.;Au TK.;Ng TB.Fong WP.;Wan DC. https://doi.org/10.1016/S0024-3205(00)00864-X
  5. 생약학회지 v.31 no.4 하고초로부터 Uesolic acid의 함량분석 김주선;강삼식;이정순;장승엽;원도희
  6. 생약학회지 v.32 no.2 발암과정 생화학적 표식자를 이용한 하고초 약침액의 암예방 활성촉진 남경수;박신화;조경희;임종국;손윤희
  7. J. Immunol. Methods v.147 Improved shot-and long-term XTT-based colorimetric cellular cytotoxicity assay for melanoma and other tumor cells. Jost, L.M.;Kirkwood, J.M.;Whiteside, T.L. https://doi.org/10.1016/S0022-1759(12)80003-2
  8. Nucleic Acids Res. v.22 A rapid and simple method for the isolation of apoptotic DNA fragments. Herrmann, M.;Lorenz, H.M.;Vollm, R.;Grunkem, M.;Woithm, W.;Kaldenm, J.R.A. https://doi.org/10.1093/nar/22.24.5506
  9. GEnes Dev. v.9 Haupt, Y.;Rowan, S.;Shaulian, E.;Vousden, K.H.;Oren, M. https://doi.org/10.1101/gad.9.17.2170
  10. Trands Cell Biol. v.11 no.12 Programmed cell death: alive and well in the new millennium. Kaufmann SH.;Hengartner MO. https://doi.org/10.1016/S0962-8924(01)02173-0
  11. Trends Mol Med. v.7 no.7 Apoptosis-regulating proteins as targets for drug discovery. Reed JC. https://doi.org/10.1016/S1471-4914(01)02026-3