Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Salviae Multiorrhizae Radix on The Cultured Mouse Hippocampal Neurons Damaged by Reactive Oxygen Species

단삼이 활성산소에 의하여 손상된 배양 해마신경세포에 미치는 영향

  • 이병찬 (원광대학교 의과대학, 원광의과학연구소) ;
  • 한선희 (원광보건대학) ;
  • 송인영 (목포과학대학) ;
  • 이강창 (원광대학교 한의학전문대학원)
  • Published : 2003.08.01
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Abstract

In order to evaluate the cytotoxic effect of reactive oxygen species(AOS), the cell viability was measured by MTT assay after cultured mouse hippocampal neurons were treated with various concentrations of xanthine oxidase(XO) and hypoxanthine (HX) for 5 hours. And also, the protective effect of Salviae Mutiorrhizae Radix(SMR) on XO/HX-induced neurotoxicity was examined in these cultures. XO/HX significantly decreased cell viability in dose-and time dependent manners when cultured mouse hippocampal neurons were treated with 5～40 mU/ml XO for 5 hours. In the protective effect of SMA, SMR increased cell viability dose-dependently after cultured mouse hippocampal neurons were preincubated with 30～120 ㎍/ml SMR for 2 hours. From these results, it is suggested that XO/HX is toxic on cultured mouse hippocampal neurons, and herbe medicine such as SMR is very effective in blocking the cytotoxicity induced by AOS.

Keywords

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