동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제17권4호
  • /
  • Pages.952-957
  • /
  • 2003
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

XO/HX에 의해 손상된 배양 심근세포에 대한 지실해백계지탕과 구성약물 추출물의 방어효과

Protective Effects of Jisilhaebaekgyeji-tang and Constituents Extract on Cultured Rat Myocardial Cell treated by XO/HX

  • 장승호 (원광대학교 한의과대학 생리학교실) ;
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 김인수 (원광대학교 한의과대학 생리학교실) ;
  • 강길성 (원광대학교 한의과대학 생리학교실) ;
  • 김인규 (원광대학교 한의과대학 생리학교실) ;
  • 김인섭 (원광대학교 한의과대학 생리학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Jang Seung Ho (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kwon Kang Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim In Su (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kang Gil Seong (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim In Gyu (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim In Seob (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
  • 발행 : 2003.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

To certify the protective effect of herbal medicine against oxygen free radical-induced myocardiotoxicity, cytotoxicity was measured using LDH activity and TBARS assay in the presence of Jisilhaebaekgyejitang(JHGT) extracts or single constituents of this prescription, In the present study, xanthine oxidase/hypoxanthine (XO/HX) resulted in a cell damage such as increases in LDH activity in culture medium and lipid peroxidation in cultured myocardial cells. In the effect of JHGT extract and its single constituents, which are Fructus Ponciri Seu Aurantii Immaturus (FPSAI), Cortex Magnoliae Officinalis (CMO), Bulbus Allii Macrostemi (BAM), Ramulus Cinnamomi (RC) and Fructus Trichosanthis (FT), they showed the prevention from the XO/HX-induced cardiotoxicity by the decrease of LDH activity and lipid peroxidation. From these results, they show that XO/HX is cardiotoxic in cultured myocardial cells derived from neonatal rat, and it suggests that JHGT, FPSAI, PT, CMO, BAM, RC and FT extracts are positively effective in the blocking in XO/HX-induced cardiotoxicity.

키워드

참고문헌

  1. J. Chin. Med. v.9 Certain progress in the treatment of coronary heart disease with traditional medicinal plants in china, Amer. Chen K. https://doi.org/10.1142/S0192415X81000251
  2. 東醫心系內科學(上) 全國韓醫科大學 心系內科學敎室 編
  3. (正統)금궤요략 醫學硏究社 譯編
  4. 금궤요략 李克光 主編
  5. (國譯)금궤요략 李東建 編著
  6. 금궤요략 再先德 主編
  7. 금궤요략탕증논치 李文瑞 主編
  8. 東醫心系內科學 具本泓;李京變;裵亨變;金永錫;李源哲 (共編著)
  9. 方劑學 李尙仁;金東傑;李暎鐘;盧昇鉉;朱榮丞 (共編譯)
  10. Oxygen free radical involvement in ischemia and reperfusion injury to brain. v.88 Cao W.;Carney J. M.;Duchon A.;Floyd R. A.;Chevion M.
  11. J. Neurochem. v.51 Excitatory amino acid release from rat hippocampal slices as a consequence of free-radical formation Pellegrini-Giampietro D. E.;Cherici G.;Alesiani M.;Carrla V.;Moroni F. https://doi.org/10.1111/j.1471-4159.1988.tb01187.x
  12. J. Neurosci. v.10 Excitatory amino acid and free radical formation may cooperate in the genesis of ischemia- induced neuronal damage, Pellegrini-Giampietro D. E.;Cherici G.;Alesiani M.; Carrla V.;Moroni F. https://doi.org/10.1523/JNEUROSCI.10-03-01035.1990
  13. J. Biol. Chem. v.245 Quantitative aspects of the production of superoxide anion radical by milk xanthine oxidase. Fridovich I.
  14. J. Biol. Chem. v.252 Liposome oxidation and erythrocyte lysis by enzymatically generated superoxideand hydrogen peroxide. Killogg E. W.;Fridovich I.
  15. Proc. Roy. Soc. v.147 The catalytic decomposition of hydrogen peroxide by anion salts. Harber F.;Weiss J.
  16. J. Biol. Chem. v.259 Iron-catalyzed hydroxyl radical formation: Stringent requirement for free iron coordination site. Graf E.;'Mahoney J. R.;Bryant R. G.;Eaton J. W.
  17. 대한동의생리병리학회지 v.15 no.4 失笑散 전탕액이 XO/HX에 의해 손상된 배양 심근세포 박동수에 미치는 영향 한동훈;권강범;황인진;김우경;김희찬;오광수;이호승;류도곤
  18. 대한동의생리병리학회지 v.15 no.4 丹參飮 전탕액이 산소자유기에 의해 손상된 심근세포의 LDH 활성도에 미치는 영향 손창식;권강범;정종선;황인진;김우경;김희찬;오광수;이호승;류도곤
  19. 대한동의생리병리학회지 v.15 no.5 甘豆湯 및 加味甘豆湯 전탕액이 배양 심근세포의 LDH 활성도에 미치는 영향 박준배;권강범;김우경;오광수;김인규;이호섭;기영운;금경수;류도곤
  20. 대한동의생리병리학회지 v.15 no.5 失笑散 전탕액과 구성약물이 배양 심근세포의 LDH 활성도에 미치는 영향 한동훈;권강범;김우경;오광수;김인규;류도곤
  21. 대한동의생리병리학회지 v.16 no.2 과루해백반하탕 추출물이 배양 심근세포의 박동수와 LDH 활성도에 미치는 영향 안효창;권강범;박은영;장승호;류도곤
  22. 대한동의생리병리학회지 v.16 no.2 手拈散 전탕액이 배양심근세포에 미치는 영향 전영석;권강범;박은영;성은경;박승택;류도곤
  23. 대한동의생리병리학회지 v.15 no.2 丹參飮전탕액이 心筋細胞 박동수에 미치는 영향 손창식;권강범;김상범;이호승;이호섭;서은아;류도곤
  24. 대한동의생리병리학회지 v.15 no.3 甘豆湯 및 加味甘豆湯 전탕액이 배양 심근세포의 총단백질량에 미치는 영향 박준배;권강범;이호승;김희찬;김우경;오광수;류도곤
  25. Methods in enzymology v.52 Microsomal lipid peroxidation. Buege J. A.;Aust S. D.
  26. Circulation v.72 Enhancement of recovery of myocardial function by oxygen free radical scavengers after reversible regional ischemia. Myers M. L.;Bolli R.;Lekich R. F.;Hartley C. J.; Roberts R. https://doi.org/10.1161/01.CIR.72.4.915
  27. Sci. v.201 The biology of oxygen radicals. Fridovich, I. https://doi.org/10.1126/science.210504
  28. Life Sci. v.34 Pharmacology of free radicals: Recent views on their action to inflammatory mechanism. Hertz F.;Cloarec A. https://doi.org/10.1016/0024-3205(84)90378-3
  29. Ann. Int. Med. v.93 Oxygen metabolism and the toxic properties of phagocytes. Klebanoff S.J. https://doi.org/10.7326/0003-4819-93-3-480
  30. Pharmacol. Rev. v.33 no.4 Free radicals in pharmacology and toxicology-Selected topocs. Mason R. P.;Chignell C. F.
  31. Ann. Int. Med. v.89 The biology and pathology of oxygen free radicals. McCord J. M.;Fridovich I. https://doi.org/10.7326/0003-4819-89-1-122