Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Multiple Shoots Regeneration and In vitro Bulblet Formation from Garlic Callus

  • Kim Soon-Seob (Dept. of Applied Life Sciences, Andong National University) ;
  • Guo De-Ping (Dept. of Horticulture, Coll. of Agriculture and Biotechnology, Zhejiang University) ;
  • Jung Do-Cheol (Dept. of Plant Resources, Sangju National University) ;
  • Kwon Soon-Tae (Dept. of Applied Life Sciences, Andong National University)
  • Published : 2003.06.01
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Abstract

The leaf segments of garlic (Allium sativum L.) were cultured in vitro and determined optimal concentration of plant growth regulators and sugars for callus induction, multiple shoots regeneration and in vitro bulblet formation. Highest yield of callus was observed in the leaf segment culture on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-D, 30 g/L sucrose and 8 g/L agar. Regeneration rate of multiple shoots from callus was high in the MS medium supplemented with kinetin 3.0 + NAA 3.0 mg/L or SA 1.0 + NAA 3.0 mg/L, containing 30 g/L sucrose. High rate of bulblet formation was observed as the concentration of jasmonic acid increased from 0.5 to 2.0 mg/L in medium, whereas addition of gibberellic acid significantly suppressed bulblet formation. The rate of in vitro bulbing was as high as $96\%$ in MS medium supplemented with 2.0 mg/L jasmonic acid and 120 g/l sucrose after two month culture at $25{\pm}1^{\circ}C$ under 16 hours day light.

Keywords

References

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