Interaction Between time of Nodal Explant Collection and Growth Regulators Determines the Efficiency of Morus alba Micropropagation

  • Hassanein A.M. (Genetics Laboratory, Faculty of Science, South Valley University) ;
  • Galal A.A. (Genetics Laboratory, Faculty of Science, South Valley University) ;
  • Azooz M.M. (Botany Department, Faculty of Science, South Valley University)
  • Published : 2003.12.01

Abstract

The hormonal requirement suiting micropropagation of Morus alba during any season throughout the year was studied. Sprouting frequency from axillary buds of M. alba was greatly influenced by the time of explant collection, the highest value was achieved when nodal explants were collected at the end of bud dormancy period (late in March) and cultured on Murashige and Skoog (MS) medium supplemented with low concentration (0.5 mg/L) of BAP, kinetin or IBA (85-68%). In addition, they showed higher axillary bud sprouting on growth-regulators-free medium (49%) than others collected in autumn or winter and cultured on medium supplemented with various growth regulators (47-48%). Regardless of that period, young explants with greenish buds collected in summer exhibiting high sprouting frequency (66%) on MS medium supplemented with 0.5 mg/L kinetin and 0.5 mg/L GA3. Shoot multiplication via adventitious bud formation was achieved when the nodal explants were cultured on MS medium supplemented with 2 mg/L BAP and 0.2 mg/L IBA. Further multiplication via nodal explants of in vitro grown shoots was obtained on MS medium supplemented with 0.5 mglL BAP and 0.5 mg/L GA3. While half strength MS medium supplemented with low concentration (0.5 mg/L) of IBA, IAA or 2,4-D stimulated adventitious root formation, IBA was the best. After transfer the plantlets to the soil, acclimatization for three weeks was essential prerequisite for survival in high frequency (92%). Peroxidase activity is related to break of bud dormancy where maximum enzyme activity was detected when the lateral buds were induced to commence growth under field condition (early in spring) or in vitro.

Keywords

References

  1. Physiol. Plant v.30 Growth and dormancy cycles in Citrus bud cultures and their hrmonal control Altman,A.;Goren,R.
  2. Annali Di Botanica v.15 Micropropagation of mulberry (Morus alba L.) Anuradha,M.;Pullaiah,T.
  3. Plant growth regulation v.33 no.215-219 Changes in peroxidase activity and level of phenolic compounds during light-induced plantlet regeneration from Eucalyptus camaldulensis Dehn Arezki,O.;Boxus,P.;Kevers,C.;Gaspar,T.
  4. Plant Cell, Tissue and Organ Cult. v.66 Effect of genotype, explant type and grwoth regulators on organogenesis in Morus Alba Bhau,B.S.;Wakhlu,A.K.
  5. Plant Cell, tissue and Organ cult. v.35 Relation between peroxidases and budding in date palm tissues cultured in vitro Booij,I.;Monfort,S.;Macheix,S.
  6. Bio Plant v.32 Stimulation of the regeneration capacity of tree shot segment explants in vitro Civinova,B.;Sladsky,Z.
  7. Proceeding of the national seminar on silk research and development Mulberry taxonomy, cytogenetics and breeding Das,B.C.
  8. Plant Physiology Deylin,M.R.;Witham,H.F.
  9. Plant Cell Biotechnology Endress,R.
  10. Acta Agrono Hungar v.47 Phenol-oxidizing isoenzymes, malate dehyderogenase patterns and organogenesis of Solanum nigrumas affected by light treatments Hassanein,A.M.;Ahmed,A.M.;Abed-Hafez;Soltan,D.M.
  11. Biol Plant v.42 Isoenzymes expression during root and shoot organogenesis of Solanum nigrum Hassanein,A.M.;Ahmed,A.M.;Abdel-Hafez;Soltan,D.M.
  12. Plant Cell Rep v.11 Micropriopagation of Morus laevigata wall, frommature trees Hossain,M.;Rahman,S.M.S.;Zaman,A.M.;Joarder,O.I.;Islam,R.
  13. Handbook fo Plant Cell Culture Meristem, shoot tip and bud cultures. Hu,C.Y.;Wang,P.J.;Evans,D.A.(ed);Sharp,W.R.(ed);Ammirato,P.V.(ed);Yamada,Y.(ed)
  14. Plant Cell Rep v.8 In vitro propagation through axillary bud multiplication in different mulberry genotypes Jain,A.K.;Dandin,S.B.;Segupta,K.
  15. Sericologia v.37 Adventitious shoot formation and plant regeneration from callus cultures of mulberry(Morus albaL.) Kathirayan,K.;Shajahan,A.;Ganapathi,A.
  16. Z. Phlanzenphysiol v.101 Organogenesis in habituated sugar beat callus: Auxin content and protectors, peroxidase pattern and inhibiotrs kevers,C.;Coumans,M.D.E.;Greef,W.;Jacobs,M.;Gaspar,T.
  17. Plant Cell Rep v.4 Regeneration of plants from the culture of leaves and axillary buds in mulberry(morus alba L.) Mhatre,M.;Bapat,V.A.;Rao,P.S.
  18. Physiol Plant v.15 A revised medium for rapid growth and bioassay with tobacco tissue culture Murashige,T.;Skoog,F.
  19. Physiol Plant v.86 Appearance of peroxidase isoenzymes in floral-initiated shoot apices of Pharbitis nil Nakanishi,F.;Fujii,T.
  20. Proc Indian Natl Sci Acad v.55 Regeneration of plantlets from callus of stem segments of mature plants of Morus alba L. Narayan,P.;Chakraboty,S.;SubbaRao,G.
  21. J. Seric Sci Jpn v.4 Studies on in vitro culture of excised bud in mulbery tree.∥. Effect of growth substances on the development of shoots from bud Oka,S.;Ohyama,K.
  22. Cell and Tissue culture in Forestry v.3 Mulberry Ohyaman,K.;Durjan,D.S.;Bonga,J.(ed);Durjan,D.S.(ed)
  23. Plant Cell Rep v.16 Rapid clonal propagation of three mulberries Morus alba cathayana Itemsl., M. Ihou Koiz and M. serrata Roxb., through in vitro culture of apicla shoot buds and modal explants from mature trees Pattnail,S.I.K.;Chand,P.K.
  24. Plant Cell Tissue and Organ cult. v.60 Morphogenic response of the alginate-encapsulated axillary buds from in vitro shoot cultures of six mulberries Pathaik,S.;Chand,P.K.
  25. Plant Cell, Tissue and Organ cult. v.45 In vitro micropropagation from nodal segments of Cleistnthus collinus Ouraishi,A.;Koche,V.A.;Mishra,S.K.
  26. Micropropation of Woody Plants Micropropagation of sandalwood(Santalum album L.) Rao,P.S.;Bapat,V.A.;Ahuja,M.R.
  27. Scientia Hortic v.42 Propagation of mulberry(Morus alba L.) Thrugh nodal segments SharmaKK;ThorpeTA
  28. Physiol Plant v.44 Isoperoxidseas in epidermal layers of tobacco and changes during organ formation i vitro Thorpe,T.A.;Tran,Thanh,Van,M.;Gaspar,T.
  29. Scientia Hortic v.80 Clonal propagation of mulberry (Morus indica L. cultivars M-5) through in vitro culture of nodal explants Vjiaya,Chitra,D.S.;Padmaja,G.
  30. Scientia Hotic v.92 Seasonal influence on axillary bud sprouting and micropropagation of elite cultivars of mulberry Vijya,Chitra,D.S.;Padmaja,G.
  31. A review. Sericologia v.41 Tissue cultures in mulberry Wakhlu,A.K.;Bhau,B.S.
  32. Physiol Plant v.88 Changes in peroxidase activity and in peroxidase isoenzyme in carrot callus Wakamatsu,K.;Takahama,U.
  33. Scientia Hort v.44 Micropropagation of Morus nigra L. From shoot tip and nodal explants of mature trees YadavU;MadanL;JaiswalVS
  34. Scientia Hort v.44 Micropropagation of Morus nigra L. From shoot tip and nodal explants of mature trees Yadav,U.;Madan,L.;Jaiswal,V.S.
  35. Acta Hort v.314 Cardiman M Research on in vitro propagation of mulberry (Morus alba L.) Ponchia,G.