Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Somatic Embryogenesis from In Vitro Grown Leaf Explants of Rosa hybrida L.

  • Kim Chang-Kil (Department of Horticulture, Sangju National Univ.) ;
  • Chung Jae-Dong (Department of Horticulture, Kyungpook National Univ.) ;
  • Jee Sun-Ok (Division of Life Sciences, Joongbu University)
  • Published : 2003.09.01
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Abstract

Somatic embryogenesis was initiated from in vitro grown leaf explants of rose following an induction period of four weeks on MS basal medium supplemented with auxin and several subcultures on MS medium with cytokinin. '4th of July' showed the highest regeneration frequencies on 1 mg/L NAA followed by culture on medium with 4 mg/L zeatin. The embryogenic callus was propagated on MS medium with NAA, zeatin and $GA_3$. Germination of somatic embryos was achieved on MS medium with 1 mg/L BA. Somatic embryo derived plantlets were hardened and successfully transferred to the greenhouse.

Keywords

References

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