Mycobiology

The Korean Society of Mycology (한국균학회)
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ITS Primers with Enhanced Specificity to Detect the Ectomycorrhizal Fungi in the Roots of Wood Plants

  • Kim, Dong-Hun (Graduate School, Biological Science and Education, Korea National University of Education) ;
  • Chung, Hung-Chae (Graduate School, Biological Science and Education, Korea National University of Education) ;
  • Ohga, Shoji (Laboratory of Forest Resources Management, Department of Forest and Forest Products Science, Faculty of Agriculture, Kyushu University) ;
  • Lee, Sang-Sun (Graduate School, Biological Science and Education, Korea National University of Education)
  • Published : 2003.03.31
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Abstract

With universal primer ITS1-F, the specific DHJ2 primer was developed to detect the Ectomycorrhizal(ECM) root tips in soil and to identify the species of ECM fungi, as based on DNA sequences of rDNA stored in GeneBank of NCBI. This primer was designed with the common sites of rDNA of Amanita and Boletus, and was also designed with several DNA programs provided by NCBI. The DNA fragments synthesized by PCR were calculated to be 1,000 to 1,200 bps of DNA located to 18s to 28s rDNA to contain two variable sites of ITS, indicating much diversities for specific species or ecotypes of ECM fungi. The primer DHJ2 reacted with the genomic DNA's extracted from the tissues of basidiocarp at the rate of 73 of 80 fungi collected produced single bands with a 1,100 bps length. The DNA fragment synthesized with the genomic DNA that extracted from eight ECM tips of Pinus densiflora was confirmed and analysized to the rDNAs of ECM in full sequences, and informed to be a ECM fungal species in the forest.

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References

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