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The Analysis of Cyanobacterial Neurotoxins by High-Performance Liquid Chromatography-Mass Spectrometry


초록

Cyanobacteria were dominant from June to September in the Nakdong River and the Hoedong Reservoir. Microcystis aeruginosa was dominant from June to September; Anabaena flos-aquae from June to August and Aphanizomenon flos-aquae from July to August. Cyanobacterial neurotoxins, Anatoxin-a and saxitoxin were analyzed by electrospray ionization-mass spectrometry with strains of Aphanizomenon flos-aquae NIES-81 and Anabaena flosaquae NIER-10002. Anatoxin-a was not detected from the cultured Anabaena flos-aquae nor from the wild samples. Low levels of saxitoxin were detected in the cultured Aphanizomenon flos-aquae however, those of field samples were below the detection limit.

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참고문헌

  1. Alam M., Ikawa M., Sasner J.J.Jr,. and Sawyer P.J. 1973. Purification of Aphanizomenon flos-aquae toxin and its chemical and physiological properties. Toxicon 11: 65-72 https://doi.org/10.1016/0041-0101(73)90154-2
  2. AOAC. 1995. In official methods of analysis, 16th ed., vol. 2. Association of official analytical chemists, Arlington, Va. Method 959.08, Chapter 49, pp. 46-48
  3. Baker P.D. and Humpage A.R. 1994. Toxicity associated with commonly occurring cyanobacteria in surface waters of the Murray-Darling Basin. Aust. J. Mar. Freshwater Res. 45: 773-786 https://doi.org/10.1071/MF9940773
  4. Bumke-Vogt C, Mailahn W. and Chorus I. 1999. Anatoxin-aand neurotoxic cyanobacteria in German lakes and reservoirs. John Willy & Sons. Inc. pp. 117-125
  5. Carmichael W.W. 1992. Cyanobacteria secondary metabolitesthe cyanotoxins. J. Appl. Bacteriol. 72: 445-459 https://doi.org/10.1111/j.1365-2672.1992.tb01858.x
  6. Carmichael W.W., Evans W.R., Yin Q.Q., Bell P. and Moczydlowski E. 1997. Evidence for paralytic shellfish poisons in the freshwater cyanobacterium Lyngbya wollei (Farlow ex Gomont) comb. nov. Appl. Environ. Microbiol. 63: 3104-3110
  7. Choi A.R., Park J.H. and Lee J.A. 2002. Population dynamics and the toxin of Anabaena in the lower Naktong river. Algae 17: 95-104 https://doi.org/10.4490/ALGAE.2002.17.2.095
  8. Codd G.A., Jefferies T.M., Keevil C.W. and Potter E. 1994. Detection methodsfor cyanobacterial toxins. Cambridge, UK pp.25-33
  9. Falconer I.R. 1993. Mechanism of toxicity of cyclic peptide toxins from blue-green algae. In: Falconer I.R. (ed.), Algal toxins in seafood and drinking water.Academic Press. San Diego. pp.176-186
  10. Falconer I.R. 1999. An overview of problems caused by toxic blue-green algae (cyanobacteria) in drinking and recreational water. Environ. Toxicol. 14: 5-12
  11. Ha K. 1999. Phytoplankton community dynamics and Microcystis bloom development in a hypertrophic river (Nakdong River, Korea). Ph. D. dissertation. Pusan National University, 140 pp
  12. Harada K.I., Ohtani I., Iwamoto K, Suzuki M., Watanabe M.F., Watanabe M. and Terao K 1994. Isolation of cylindrosperrmopsin from a cyanobacterium Umezakia natans and its screening method. Toxicon 32: 73-84 https://doi.org/10.1016/0041-0101(94)90023-X
  13. Himberg K 1989. Determination of anatoxin-a, the neurotoxin of Anabaenaflos·aquae cyanobacyerium, in algae and water by gas chromatography-mass spectrometry. J. Chromatography 481: 358-362 https://doi.org/10.1016/S0021-9673(01)96779-2
  14. Humpage A.R., Rositano J., Bretag A., Brown R., Baker P., Nicholson B.C. and Steffensen. D.A 1994. Paralytic shellfish poisons from Australian cyanobacterial blooms. Aust, J. Mar.freshwater Res. 45: 761-771 https://doi.org/10.1071/MF9940761
  15. Ikawa M., Wegener K., Foxall T. L. and Sasner J.J.Jr. 1982. Comparison of the toxins of the blue-green alga Aphanizonicnon [los-uquac with the Gonyaulax toxins. Toxicon 20: 747-752 https://doi.org/10.1016/0041-0101(82)90122-2
  16. Jaime E., Hummert c.. Hess P. and Luckas B. 2001. Determination of paralytic shellfish poisoning toxins by high-performance ion-exchange chromatography. J. Chromatography A 929: 43-49 https://doi.org/10.1016/S0021-9673(01)01181-5
  17. Kaas H. and Henriksen P. 2000. Saxitoxin (psp toxins) in Danish Lakes. Wat. Res. 34: 2089-2097 https://doi.org/10.1016/S0043-1354(99)00372-3
  18. Lagos N., Onodera H., Zagatto P.A., Andrinolo D., Azevedo S.M.F.Q. and Oshima Y. 1999. The first evidence of paralytic shellfish toxins in the freshwater cyanobacterium Culindrospermopsis raciborskii, isolated from Brazil. Toxicon 37: 1359-1373 https://doi.org/10.1016/S0041-0101(99)00080-X
  19. Lee J.A., Choi A.R. and Watanabe M. 1997. Toxonomic implication of the genus Microcystis (cyanophyceae) from the Naktong River. Algae 12: 167-176
  20. Mahmood N.A and Carmichael W.W. 1986. Paralytic shellfish poisons produced by the freshwater cyanobacterium Aphanizornenon flos-aquae NH-5. Toxicon 24: 175-186 https://doi.org/10.1016/0041-0101(86)90120-0
  21. Namera A., So A and Pawliszyn J. 2002. Analysis of anatoxin-a in aqueous samples by solid-phase microextraction coupled to high-performance liquid chromatography with fluorescence detection and on-fiber derivatization. J. Chromatography A 963: 295-302 https://doi.org/10.1016/S0021-9673(02)00648-9
  22. Ohsima Y. 1995. Post-column derivatization HPLC methods for paralytic shellfish poisons. In: Hallegraeff G.M., Anderson D.M. and Cembella A.D.(eds), Manual on harntiul marine mieroalgae. IOC manuals and guides No. 33. UNESCO. pp. 81-94
  23. Onodera H., Satake M., Oshima Y., Yasu rno to T. and Carmichael W.W. 1997. New saxitoxin analogues from the freshwater filamentous cyanobacterium Lyngbya iootlci, Nat. Toxins 5: 146-151
  24. Park H.D., Kim B., Kim E. and Okino T. 1998. Hepatotoxic microcystins and neurotoxic anatoxin-a in cyanobacterial blooms from Korean Lakes. Environ. Toxicol. Water Qual. 13: 225-234
  25. Pereira P., Onodera P.H., Andrinolo D., Franca S., Araujo F., Lagos N. and Oshima Y. 2000. Paralytic shellfish toxins in the freshwater cyanobacterium Aphanizomenon flosaquae, isolated from Montargil reservoir, Portugal. Toxicon 38: 1689-702 https://doi.org/10.1016/S0041-0101(00)00100-8
  26. Powell M.W. 1997. Analysis of anatoxin-a in aqueous samples. Chrornatographia 45: 25-28 https://doi.org/10.1007/BF02505532
  27. Sivonen K. and Jones G. 1999. Cyanobacterial toxins. In: Chorous 1. and Bartram J. (eds). Toxic cyanobacteria ill water. EFN Spon., London. pp. 41-111
  28. Steffensen D.A., Humpage A. R., Rositano J., Bretag A.H., Brown R., Baker r.o. and Nicholson B.C. 1994. Neurotoxin from Australian Anabaena. In: Codd G.A., Jefferis T.M., Keevil C.W. and Potter E. (eds), Detection methods for cyanobacterial toxins. RSC., pp. 45-50
  29. Wetzel R.G. and Likens G.E. 1991. Lirnnologicalallalysis. 2nd ed. Springer-Verlag. New York. 391 pp
  30. Zotou A and Jefferies T.M. 1999. Determination of anatoxin-a and homoanatoxin in blue-green algal extracts by highperformance liquid chromatography and gas chromatography-mass spectrometry. Analyst. 118: 753-758 https://doi.org/10.1039/an9931800753

피인용 문헌

  1. Analysis of anatoxin-a in aqueous and cyanobacterial samples from korean lakes by liquid chromatography with fluorescence detection vol.24, pp.3, 2011, https://doi.org/10.5806/AST.2011.24.3.225
  2. A review of the phylogeny, ecology and toxin production of bloom-forming Aphanizomenon spp. and related species within the Nostocales (cyanobacteria) vol.54, 2016, https://doi.org/10.1016/j.hal.2015.09.007
  3. Molecular Verification of Bloom-forming Aphanizomenon flos-aquae and Their Secondary Metabolites in the Nakdong River vol.15, pp.8, 2018, https://doi.org/10.3390/ijerph15081739