Korean Journal of Microbiology (미생물학회지)
- Volume 39 Issue 4
- /
- Pages.260-266
- /
- 2003
- /
- 0440-2413(pISSN)
- /
- 2383-9902(eISSN)
Evaluating the Impacts of Long-Term Use of Agricultural Chemicals on a Soil Ecosystem by Structural Analysis of Bacterial Community
세균군집의 구조분석을 통한 장기간 농약사용이 토양생태계에 미치는 영향 평가
- Yun, Byeong-Jun (Department of Environmental Science, Sunchon National University) ;
- Kim, Seong-Hyeon (Department of Environmental Science, Sunchon National University) ;
- Lee, Dong-Heon (Department of Life Science, Cheju National University) ;
- O, Gye-Heon (Department of Life Science, Soonchunhyang University) ;
- Gang, Hyeong-Il (Department of Environmental Science, Sunchon National University)
- Published : 2003.01.01
Abstract
In this study bacterial community was analyzed to evaluate the impacts of long-term use of agricultural chemicals on a soil ecosystem as well as to obtain fundamental data on the relationship. Sequences of 16S rRNA clones from a non-agricultural site and a tangerine orchard soil which has a history of long-term use of agricultural chemicals over 30 years were analyzed. This revealed that bacterial community containing 5 divisions and 18 genera was distributed in a tangerine orchard soil, while bacterial community containing 9 divisions and 44 genera was distributed. In a tangerine orchard soil site, the most abundant bacteria in subdivision level were placed into Proteobacteria γ group which occupied 56% of total clones. The other bacterial clones from the ocrhcard soil exposed to agricultural chemicals over 30 years were Acidobacteria group (25%), Fimicutes group (5%), Planctomycetes group (2%), Proteobacteria α (1%), δ group (1%), and Cyanobacteria group (1%). Whereas, the clones were from the non-agricultural site were distributed among the division or subdivision Acidobacteria group (14%), Planctomycetes group (13%), Proteobacteria α (10%), β (9%), δ (9%), Fimicutes group (8%), Verrucomicrobia group (8%), Actinobacteria group (6%), Proteobacteria γ group (3%), Bacteroidetes group (3%), Gemmatimonadetes group (3%), and Cyanobacteria group (1%). This finding suggests the possibility that long-term application of agricultural chemicals or fertilizers on a tangerine orchard might result in drastic reduction or alteration in the composition of the bacterial community in the contaminated soil site.
본 연구는 장기간 동안 지속적인 농약의 사용이 토양생태계에 미치는 영향을 세균군집의 구조분석을 통하여 그 관련성을 평가하고, 이에 대한 기초 자료를 얻고자 수행하였다. 30년 이상 매년 수시로 농약을 사용하여온 제주지역의 한 감귤원 토양과 비농지 토양에 존재하는 세균군집을 16S rRNA clonal library로부터 얻은 각 100 개 클론의 유전자 염기서열을 기초로 하여 비교 분석한 결과 지속적으로 농약을 사용해온 감귤원 토양에서는 3개의 아문(Proteobacteria