한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제18권4호
  • /
  • Pages.177-182
  • /
  • 2003
  • /
  • 1229-1153(pISSN)
  • /
  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
권호 표지

삼백초 건초 추출물의 간세포 보호 효과 연구

Protective Effects of Saururus chinensis Baill Extracts on Liver Cell

  • 하배진 (신라대학교 공과대학 생명공학) ;
  • 하종명 (신라대학교 공과대학 생명공학) ;
  • 이상현 (신라대학교 공과대학 생명공학) ;
  • 이진영 (신라대학교 공과대학 생명공학) ;
  • 박선이 (아주대학교 의과대학 생화학)
  • Ha, Bae-Jin (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Ha, Jong-Myung (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Lee, Sang-Hyeon (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Lee, Jin-Young (Department of Bioscience and Biotechnology, College of Engineering, Silla University) ;
  • Park, Sung-Yi (Department of BIochemistry, Ajou University School of Medicine)
  • 발행 : 2003.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

삼백초가 $CCl_4$ 투여로 유발된 rat의 지질대사에 미치는 효과를 알아보기 위해 NCT, CTA, SCT군으로 각각 10마리씩 나누어 NCT군과 CTA군은 0.9% saline을 SCT군은 삼백초를 100 mg/kg이 되게 2주 동안 매일 복강내에 투여하였다. 15일째 $CCl_4$를 CTA군과 SCT군에 투여하였다. 희생시켜서 혈청과 간의 지질대사를 관찰한 결과는 다음과 같다. 1. 혈청 중의 AST와 ALT의 활성도에서 SCT군은 CTA군에 비교해서 각각 43.18%, 96.05% 억제되었다. 2. Total cholesterol에서 삼백초를 투여한 SCT군은 NCT군 비교해서 0.95배 증가하였으며, 혈청중의 HDL-cholesterol은 SST군이 CTA군과 비교해서 2.63배 증가하였고 90.00%로 억제되었다. LDL-cholesterol에서는 SCT군이 CTA군보다 0.73배 감소하였다. 3. 혈청중의 TG의 함량은 CTA군이 NCT군에 비해 1.53배 증가하였다. CTA군에 비해 SCT군은 0.93배 감소하였으며 19.00%로 억제되었다. 4. 간조직 중의 MDA량은 SCT군이 CTA군에 비해 0.85배 감소하였고, 33.00%의 억제율을 보였다.

Saururus chinensis Baill (Saururaceae) is a perennial plant that has been used for therapy of edema, jaundice and gonorrhea in Korean folk medicine. This study was carried out to investigate the inhibitory effects of Saururus chinensis Baill(SCB) on hepatoprotective effects in accutely Carbone Tetrachloride ($CCl_4$) treated rats. Saururus chinensis Baill (100 mg/kg) was administered into rats intraperitoneally for 2 weeks, after the injection of CCl4 (3.3 ml/kg). We dxamined the biochemical parameters by measuring the levels of Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Total cholesterol, Triglyceride (TG), HDL-cholesterol, and LDL-cholesterol in serum and Malonedialdehyde (MDA) in liver tissue of rats. cholesterol was siginificantly elevated in $CCl_4$-treated abnormal group (CTA). The higher level of HDL-cholesterol was found is Saururus chinensis Baill and CCl4 administered group (SCT), which showed the lower levels of Total-cholesterol and LDL-cholesterol. TG content in the SCT was decreased compared to CTA by 0.93 times. MDA content in the SCT was inhibited compared to CTA by 33.00%. These findings indicate that Saururus chinensis. Baill may have a protective effect against CCl4-treated hepatotoxicity in rats.

키워드

참고문헌

  1. Ashburn, L.L., Endicott, K.M. Daft, F.S. and Little, R.D.: The nonportal distribution of the trabecule in dietary cirvhosis of mouse and huinea pigs. Am. J. Path. 23, 159, (1947)
  2. 정덕상: Saururus Chinesis 의 지방산 및 아미노산의 성분연구. cheju univ. Jour. (Natural Sci.), 35, 111, (1992)
  3. 전길환, 신민교, 송호준: 三白草 腹腔 大食細胞로부터 Nitric Oxide(NO) 형태변환에 대한 연구. 대한학의학회지, 19-23, (1998) (1998)
  4. 이상호, 박철우, 박경아, 이영춘, 김무남, 하영래: 삼백초 Hexane 분획물의 Heterocylic Amine 돌연변이성 조정효과. 한국환경성돌연변이발암원학회지, 18, 26, (1998)
  5. 이인선: 삼백초 열수출물의 항암 및 세포독성 저해 효과. 농산물저장유통학회지 8, 213-216, (2001)
  6. Friedwld, W.T., Levy, R.I., Fedreicson, D.S.: Estimation of the concentration of low-density lipoprotein cholesterol in plasma, withoutwas of the preparative Ultracentrifuge. Clin Chem, 18, 499-506, (1972)
  7. Stacey, N. H., Ottenwaelder, H. and Kappus, H.: CCl4-induced lipid peroxidation in Isolated Rat Hepatocytes with Different oxygen concentrations., Toxicol. Appl. Pharmacol., 62, 421-427, (1982) https://doi.org/10.1016/0041-008X(82)90143-0
  8. Lowry. O.H., Rosenbrough. N.J., Farr, A.S. and Randall, R.J.: Protein Measurenment with folin phenol reagent, J. Biol. Chem. 193, 256, (1951)
  9. Hayes, Gabriel L. Plaa and William R. Hewitt.: Principle and methods of Toxicology, Raben Press, 407-445, (1982)
  10. Freeman, B.A. and Crapo, J.D.: Biology of diseasde: Free radicals and tissue injury, Lab. Invest., 47, 412-426 (1981) https://doi.org/10.1038/jid.1966.69
  11. Simon, R.H., Scoggin, C.M. and Patterson, D.: Hydrogen peroxide cause the fetal injury to human fibroblasts exposed to oxygen radicals, J. Bioi. Chem., 226, 7181-7187
  12. 여지영, 이영주, 한준표: 송화분 단백질이 사염화탄소를 투여한 흰쥐의 혈청에 미치는 효과, J. korean Soc. Food Nutr. 25(1) 34-38 (1996).
  13. 성인수, 김명주, 조수열: 도토리추출물이 흰쥐의 체내 지질대사에 미치는 영향, J. korean Soc. Food Sci. nutr., 25(1), 327-333 (1997)
  14. Gordon, T., Castelli, W.P., Hjortland, M.C., Kannel, W.B. and Dawber, T.R.: Higher denstiy lipoprotein as a protective factor against coronary heart diseases : the framingham study, Am. J. Med., 62, 707-714, (1997) https://doi.org/10.1016/0002-9343(77)90874-9
  15. Rhoades, G.G., Gulbrandsen, C.L, and Kagan, A.: Serum lipoproteins and coronary heart disease in a population study of Hawaii Japaness men, N. Eng. J. Med., 294, 293-297 (1976)
  16. 南質根: 地質代謝에 관여하는 因子, J. Korean Food Nutr., 15(2), 191-200 (1986)
  17. Steinbeerg, D.: Lipoproteins and atherosclerosis : a look back and a look ahead, Atherosclerosis, 3, 283-301 (1983)
  18. Gordon, T., kannel, W. B., Castelli, W. P. and Dawber, T. R.: Lipoproteins, cardiovascular disease, and death : the Framingham study, Arch. Inter. Med, 141, 1128-1132, (1981) https://doi.org/10.1001/archinte.141.9.1128
  19. 北川淸雄: 독성학, 남강당, (1982)
  20. Curtis, M. T., Gilfor, D. and Farber, J. L.: Lipid peroxidation increased the molecular order of microsomal membranes, Arch. Biochem. Biophy., 235, 644-651 (1984) https://doi.org/10.1016/0003-9861(84)90239-X
  21. Takeda, S., Funo, S., Ilzuka, A., Kase, Y., Arai, I, Ohkura, Y., Sudo, K., Kiuchi, N., Yoshida, C., Maeda, S., Abrade, M. and Hosoya, E.: Pharmacological studies on schizandra fruits Ill. Effects of wuweizisu C, a ligan component of schixanda fruits, on experimental liver injuries in rats. Folia Pharmacol. Japon, 85, 194-208 (1985)
  22. Noll, T. and Groot, H.: The critical steady state hypoxic conditions in carbon tetrachloride induced lipid peroxidation in rat liver microsomes, Biochem. Biophys, Acta, 795, 356-362 (1984) https://doi.org/10.1016/0005-2760(84)90086-9