Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Genetic analysis for Polymorphism of 5,10-Methylenetetrahydrofolate Reductase (MTHFR) A1298C and Infertile Males in Korea

한국인 남성 불임 환자에서 5,10-Methylenetetrahydrofolate Reductase (MTHFR) 유전자의 1298번의 다형성과의 관련성에 관한 연구

  • Jeong, Yu-Mi (Genome Research Center for Reproductive Medicine and Infertility, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Chung, Tae-Gue (Department of Urology, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Kim, Hyun-Joo (Department of Urology, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Lee, Sook-Hwan (Genome Research Center for Reproductive Medicine and Infertility, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Park, Jung-Hoon (Genome Research Center for Reproductive Medicine and Infertility, CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Kim, Nam-Keun (CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Kim, Se-Hyun (CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Cha, Kwang-Yul (CHA General Hospital, College of Medicine, Pochon CHA University) ;
  • Lee, Su-Man (Genome Research Center for Reproductive Medicine and Infertility, CHA General Hospital, College of Medicine, Pochon CHA University)
  • 정유미 (차병원 생식의학 및 불임유전체 연구센터) ;
  • 정태규 (차병원 비뇨기과) ;
  • 김현주 (차병원 비뇨기과) ;
  • 이숙환 (차병원 생식의학 및 불임유전체 연구센터) ;
  • 박정훈 (차병원 생식의학 및 불임유전체 연구센터) ;
  • 김남근 (차병원) ;
  • 김세현 (차병원) ;
  • 차광렬 (차병원) ;
  • 이수만 (차병원 생식의학 및 불임유전체 연구센터)
  • Published : 2003.12.30
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Abstract

Objective: To investigate the association of genetic background between MTHFR A1298C genotype and male infertility. Materials and Methods: We compared 377 infertile males with 396 healthy fertile males with one or more offspring. Infertile males were classified into four subtypes (281 azoospermia, 26 oligoasthenoteratozoospermia (OAT), 59 severe OAT and 11 remnants) by World Health Organization (WHO). Pyrosequencing analysis for MTHFR (methylenetetrahydrofolatereductase) A1298C variation was performed on polymerase chain reaction (PCR) product of study group. To validate pyrosequencing data of A1298C variation for randomly selected 50 samples, we compared the pyrosequencing result with the PCR-RFLP (Restriction Fragment Length Polymorphism) result of MTHFR A1298C genotype. Results: We studied MTHFR A1298C variation by pyrosequencing. A1298C variation data (1298 AC; p=0.2166 and 1298 CC; p=0.5056) of MTHFR gene was no significant difference in between fertile and infertile males. Conclusion: The genetic analysis in MTHFR gene didn't appear genetic difference in Korean fertile and infertile males. We require further study for MTHFR gene in infertile males.

Keywords

References

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