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The Pharmaceutical Society of Korea (대한약학회)
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Characteristics of Chitosanases from Aspergillus fumigatus KB-1

  • Published : 2003.11.01
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Abstract

Two chitosanases produced by Aspergillus fumigatus KB-1 were purified by ion exchange and size exclusion chromatographies. Molecular weights of chitosanases were 111.23 kDa (chitosanase I) and 23.38 kDa (chitosanase II). The N-terminal amino acid sequence of chitosanase II was determined as follows: YNLPNNLKQIYDKHKGKXSXVLAKGFTN. The optimum pH of the chitosanase I and II was 6.5 and 5.5, respectively. The optimum temperatures were $60^{\circ}C$ for chitosanase land $70^{\circ}C$ for chitosanase II. Hydrolysis products of two chitosanases were analyzed by HPLC and GPC. Chitosanase I hydrolyzed substrate to glucosamine. Chitosanase II produced chitooligosaccharides.

Keywords

References

  1. Amano, K., Hayashi, H., Araki, Y., and Ito, E., The action of lysozyme on peptidoglycan with N-unsubstituted glucosamine residues. Isolation of glycan fragments and their susceptibility to lysozyme. Eur. J. Biochem., 76, 299-307 (1977) https://doi.org/10.1111/j.1432-1033.1977.tb11596.x
  2. Bartnicki-Garcia, S., Cell wall chemistry, morphogenesis, and taxanomy of fungi. Annu. Rev. Microbiol., 22, 87-108 (1968) https://doi.org/10.1146/annurev.mi.22.100168.000511
  3. Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254 (1976) https://doi.org/10.1016/0003-2697(76)90527-3
  4. C. Y. and Li, Y. K., An Aspergillus chitosanase with potential for large-scale preparation of chitosan oligosaccharides. Biotechnol. Appl. Biochem., 32, 197-203 (2000) https://doi.org/10.1042/BA20000063
  5. Hadwiger, L. A., Fristensky, B., and Riggleman, R. C., Chitin, chitosan and related enzymes. J. P. Zikakis(ed.), New York, pp. 291-302 (1984)
  6. Hirano, S., Hirochi, K., Hayashi, K., Mikami, T., and Tachibana, H., Pharmaceutical and cosmetic uses of chitin and chitosan. Cosmetic and Pharmaceutical Polymers. Plenum, New York, pp. 95-104 (1991)
  7. Izumo, M., Nagae, S., Kawagishi, H., Mitsutomi, M., and Ohtakara, A., Action pattern of Bacillus sp. No. 7-M chitosanase on partially N-acetylated chitosan. Biosci. Biotech. Biochem., 56, 448-453 (1992) https://doi.org/10.1271/bbb.56.448
  8. Jennings, C. D., Boleyn, K., Bridges, S. R., Wood, P. J., and Anderson, J. W., A comparison of the lipid-lowering and intestinal morphological effects of cholestyramine, chitosan, and oat gum in rats. Proc. Soc. Exp. Biol. Med., 189, 13-20 (1988) https://doi.org/10.3181/00379727-189-42773
  9. Kim, S. Y., Shon, D. H., and Lee, K. H., Purification and characteristics of two type chitosanases from Aspergillus fumigatus KH-94. J. Microbiol. Biotechnol., 8, 568-574 (1998)
  10. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970) https://doi.org/10.1038/227680a0
  11. Lee, D. M., Noh, H. J., and Lee K. M., Purification and characterization of a chitinase from Cytophaga sp. HJ isolated from sea sand. J. Microbiol. Biotechnol., 9, 839-846 (1999)
  12. Muzzarelli, R. A., Tanfani, F. and Scarpini, G., Chelating, filmforming, and coagulating ability of the chitosan-glucan complex from Aspergillus niger industrial wastes. Biotechnol. Bioeng., 22, 885-896 (1980) https://doi.org/10.1002/bit.260220412
  13. Rondle, C. J. and Morgan, W. T., The determination of glucosamine and galactosamine. Biochem. J., 61, 586-589 (1955) https://doi.org/10.1042/bj0610586
  14. Shimosaka, M., NCBI's BLAST Search, Accession BAC10610 (2002)
  15. Somashekar, D. and Joseph, R., Chitosanase-properties and applications : A review. Bioresource Technol., 55, 35-45 (1996) https://doi.org/10.1016/0960-8524(95)00144-1
  16. Suzuki, K., Mikami, T., Okawa, Y., Tokoro, A., Suzuki, S., and Suzuki, M., Antitumor effect of hexa-N-acetylchitohexaose and chitohexaose. Carbohydr. Res., 151, 403-408 (1986) https://doi.org/10.1016/S0008-6215(00)90359-8
  17. Yoon, H. G., Ha, S. C., Lim, Y. H., and Cho, H. Y., New thermostable chitosanases from Bacillus sp. : Purification and characterization. J. Microbiol. Biotechnol., 8, 449-454 (1998)
  18. Zhang, X. Y., Dai, A. L., Kuroiwa, K., Kodaira, R., Nogawa, M., Shimosaka, M., and Okazaki, M., Cloning and characterization of a chitosanase gene from the koji mold Aspergillus oryzae strain IAM 2660. Biosci. Biotechnol. Biochem., 65, 977-981 (2001) https://doi.org/10.1271/bbb.65.977