KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Isolation and Characterization of Microbacterium barkeri LCa and Paenibacillus amylolyticus LCb for PVA [Poiyvinyl Alcohol］Degradation

PVA [Poiyvinyl Alcohol］분해용 균주 Microbacterium barkeri LCa 및 Paenibacillus amylolyticus LCb의 분리 및 특성 연구

  • 최광근 (광운대학교 공과대학 화학공학과) ;
  • 신종철 (광운대학교 공과대학 화학공학과) ;
  • 전현희 (광운대학교 공과대학 화학공학과) ;
  • 김상용 (한국생산기술연구원) ;
  • 류원석 (영남대학교 섬유패션학부) ;
  • 이진원 (광운대학교 공과대학 화학공학과)
  • Published : 2003.12.01
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Abstract

34 strains were isolated from dyeing wastewater in order to improve treatment efficiency of dyeing wastewater containing PVA. Two strains of them were finally selected through the PVA degrading test, and identified as Microbacterium barkeri LCa and Paenibacillus amylolyticus LCb. As a result, optimal conditions for microbial growth and PVA degradation were 30$^{\circ}C$, neutral pH, starch as a carbon source, and peptone as a nitrogen source. And it was concluded that these two strains have good ability for PVA degradation. And 90% over PVA was degraded by single culture as well as a mixed culture of 2 different strains.

염색폐수에 포함된 PVA를 생물학적으로 제거하고자 34 종의 PVA 분해용 균주를 염색폐수 및 슬러지로부터 분리하였다. 이 중 PVA 분해 시험을 거쳐 2 종의 균주를 최종분리하여 동정하여 Microbacterium barkeri LCa와 Paenibacillus amylolyticus LCb로 명명하였다. 최종분리균의 최적성장조건 및 최대분해조건을 규명해보았는데, 온도는 3$0^{\circ}C$, pH는 7, 탄소원은 starch, 그리고 질소원은 peptone으로 판명되었으며, 최적조건 하에서의 PVA 분해율은 89%를 보였다. 또한, 본 연구에서 분리한 균주는 PVA 중합도의 영향 없이 높은 분해율을 유지하는 것으로 판명되었다.

Keywords

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