Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Phospholipids from Bombycis corpus and Their Neurotrophic Effects

  • Kwon, Hak-Cheol (College of Pharmacy, SungKyunKwan University) ;
  • Jung, I-Yeon (Department of Sericulture and Entomology, National Institute Agricultural Science and Technology, RDA) ;
  • Cho, Se-Yeon (Department of Sericulture and Entomology, National Institute Agricultural Science and Technology, RDA) ;
  • Cho, Ock-Ryun (College of Pharmacy, SungKyunKwan University) ;
  • Yang, Min-Cheol (College of Pharmacy, SungKyunKwan University) ;
  • Lee, Sung-Ok (College of Pharmacy, SungKyunKwan University) ;
  • Hur, Jin-Young (Korea and Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Kim, Sun-Yeou (Korea and Graduate School of East-West Medical Science, Kyung Hee University) ;
  • Yang, Jong-Beom (Dept. of Food Science ＆ Technology, DongNam Health College) ;
  • Lee, Kang-Ro (College of Pharmacy, SungKyunKwan University)
  • Published : 2003.06.01
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Abstract

Three phospholipids (4-6) and three aromatic amines (1-3) were obtained from the methanol extract of Bombycis corpus. Based on spectral data, their structures have been elucidated as nicotiamide (1), cytidine (2), adenine (3), 1-Ο-(9Z-octadecenoyl)-2-Ο-(8Z,11Z-octadecadienoyl)-sn-glycero-3-phosphorylcholine (4), 1,2-di-Ο-hexadecanoyl-sn-glycero-3-phosphorylcholine (5) and 1,2-di-Ο-9Z-octadecenoyl-sn-glycero-3-phosphorylcholine (6). We examined the effects of compounds on synthesis of NGF in cultured astrocytes. By RT-PCR analysis, expresison of NGF mRNA in astrocytes cultured in serum-starvation increased after the addition of phospholipid (10 $\mu$M). The NGF content in the culture medium was significantly increased by compound 5, compared with the control value. These results suggest that three phospholipid compounds isolated from the methanol extract of Bombycis corpus may exert neurotrophic effects by stimulation of NGF synthesis in astrocytes.

Keywords

References

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