Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Analysis of Vasopressin-Induced $Ca^{2+}$ Increase in Rat Hepatocytes

  • Kim, Hyun-Sook (Laboratory of Pharmacology, College of Pharmacy, Pusan National University) ;
  • Fumikazu-Okajima (Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University) ;
  • Im, Dong-Soon (Laboratory of Pharmacology, College of Pharmacy, Pusan National University)
  • Published : 2003.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

To analyze vasopressin-induced $Ca^{2+}$ increase in liver cells, rat hepatocytes were isolated and attached to collagen-coated cover slips. Using fura-2, a $Ca^{2+}$-sensing dye, changes in intracellular $Ca^{2+}$ concentration by vasopressin were monitored. Results in this communication suggested that vasopressin-induced $Ca^{2+}$ increase were composed of both $Ca^{2+}$ release from internal $Ca^{2+}$ stores and influx from the plasma membrane. The $Ca^{2+}$ influx consisted of two distinguishable components. One was dependent on the presence of vasopressin and the other was not. SK＆F96365 blocked vasopressin-induced $Ca^{2+}$ influx in a dose-dependent manner. Vasopressin-induced $Ca^{2+}$ release from internal stores diminished in a primary culture of hepatocytes according to the culture time. However, changes in vasopressin-induced $Ca^{2+}$ influx across the plasma membrane differed from changes in the $Ca^{2+}$ release from internal stores, suggesting two separate signalings from receptor activation to internal stores and to the plasma membrane.

Keywords

References

  1. Barritt, G. J., Receptor-activated Ca2+ inflow in animal cells: a variety of pathways tailored to meet different intracellular Ca2+ signalling requirements. Biochem. J., 337 (Pt 2), 153-169 (1999) https://doi.org/10.1042/0264-6021:3370153
  2. Berridge, M. J., Capacitative calcium entry. Biochem. J., 312 (Pt 1), 1-11 (1995) https://doi.org/10.1042/bj3120001
  3. Boulay, G., Brown, D. M., Qin, N., Jiang, M., Dietrich, A., Zhu, M. X., Chen, Z., Birnbaumer, M., Mikoshiba K., Birnbaumer, L., Modulation of Ca(2+) entry by polypeptides of the inositol 1,4,5-trisphosphate receptor (IP3R) that bind transient receptor potential (TRP): evidence for roles of TRP and IP3R in store depletion-activated Ca(2+) entry. Proc. Natl. Acad. Sci. USA, 96, 14955-14960 (1999) https://doi.org/10.1073/pnas.96.26.14955
  4. Burgess, G. M., Godfrey, P. P., McKinney, J. S., Berridge, M. J., Irvine, R. F., Putney, J. W., Jr., The second messenger linking receptor activation to internal Ca release in liver. Nature, 309, 63-66 (1984) https://doi.org/10.1038/309063a0
  5. Fernando, K. C., Barritt, G. J., Characterisation of the inhibition of the hepatocyte receptor-activated Ca2+ inflow system by gadolinium and SK&F 96365. Biochim. Biophys. Acta, 1222, 383-389 (1994) https://doi.org/10.1016/0167-4889(94)90044-2
  6. Fernando, K. C., Barritt, G. J., Characterisation of the divalent cation channels of the hepatocyte plasma membrane receptor-activated Ca2+ inflow system using lanthanide ions. Biochim. Biophys. Acta, 1268, 97-106 (1995) https://doi.org/10.1016/0167-4889(95)00041-P
  7. Im, D. S., Fujioka, T., Katada, T., Kondo, Y., Ui, M., Okajima, F., Characterization of sphingosine 1-phosphate-induced actions and its signaling pathways in rat hepatocytes. Am. J. Physiol., 272, G1091-1099 (1997)
  8. Irvine, R. F., 'Quantal' Ca2+ release and the control of Ca2+ entry by inositol phosphates--a possible mechanism. FEBS Lett., 263, 5-9 (1990) https://doi.org/10.1016/0014-5793(90)80692-C
  9. Itoh, H., Okajima, F., Ui, M., Conversion of adrenergic mechanism from an alpha- to a beta-type during primary culture of rat hepatocytes. Accompanying decreases in the function of the inhibitory guanine nucleotide regulatory component of adenylate cyclase identified as the substrate of islet-activating protein. J. Biol. Chem., 259, 15464-15473 (1984)
  10. Kajiyama, Y., Ui, M., Switching from alpha 1- to beta-subtypes in adrenergic response during primary culture of adult-rat hepatocytes as affected by the cell-to-cell interaction through plasma membranes. Biochem. J., 303 (Pt 1), 313-321 (1994) https://doi.org/10.1042/bj3030313
  11. Kajiyama. Y., Ui. M., Differential mitogenic actions of alpha 1-and beta-adrenergic agonists on rat hepatocytes. Cell Signal, 10, 241-251 (1998) https://doi.org/10.1016/S0898-6568(97)00122-8
  12. Kiselyov, K., Mignery, G. A., Zhu, M. X., Muallem, S., The Nterminal domain of the IP3 receptor gates store-operated hTrp3 channels. Mol. Cell, 4, 423-429 (1999) https://doi.org/10.1016/S1097-2765(00)80344-5
  13. Kiselyov, K., Xu, X., Mozhayeva, G., Kuo, T., Pessah, I., Mignery, G., Zhu, X., Birnbaumer, L., Muallem, S., Functional interaction between InsP3 receptors and store-operated Htrp3 channels. Nature, 396, 478-482 (1998) https://doi.org/10.1038/24890
  14. Lenz, T., Kleineke, J. W., Hormone-induced rise in cytosolic Ca2+ in axolotl hepatocytes: properties of the Ca2+ influx channel. Am. J. Physiol., 273, C1526-1532 (1997) https://doi.org/10.1152/ajpcell.1997.273.5.C1526
  15. Merritt, J. E., Armstrong, W. P., Benham, C. D., Hallam, T. J., Jacob, R., Jaxa-Chamiec, A., Leigh, B. K., McCarthy, S. A., Moores, K. E., Rink, T. J., SK&F 96365, a novel inhibitor of receptor-mediated calcium entry. Biochem. J., 271, 515-522 (1990) https://doi.org/10.1042/bj2710515
  16. Okajima, F., Ui, M., Predominance of beta-adrenergic over alpha-adrenergic receptor functions involved in phosphorylase activation in liver cells of cholestatic rats. Arch. Biochem. Biophys., 230, 640-651 (1984) https://doi.org/10.1016/0003-9861(84)90445-4
  17. Putney, J. W., Jr., Broad, L. M., Braun, F. J., Lievremont, J. P., Bird, G. S., Mechanisms of capacitative calcium entry. J. Cell Sci., 114, 2223-2229 (2001)
  18. Randriamampita, C., Tsien, R. Y., Emptying of intracellular Ca2+ stores releases a novel small messenger that stimulates Ca2+ influx. Nature, 364, 809-814 (1993) https://doi.org/10.1038/364809a0
  19. Rhee, S. G., Regulation of phosphoinositide-specific phospholipase C. Annu. Rev. Biochem., 70, 281-312 (2001) https://doi.org/10.1146/annurev.biochem.70.1.281
  20. Zubov, A. I., Kaznacheeva, E. V., Nikolaev, A. V., Alexeenko, V. A., Kiselyov, K., Muallem, S., Mozhayeva, G. N., Regulation of the miniature plasma membrane Ca(2+) channel I(min) by inositol 1,4,5-trisphosphate receptors. J. Biol. Chem., 274, 25983-25985 (1999) https://doi.org/10.1074/jbc.274.37.25983