YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

A Role of Neutrophils in Anti-Candida Monoclonal Antibody Protection Against Vaginal Infection due to Candida albicans

캔디다질염에 효과가 있는 단항체에 대한 호중구의 역할

  • 한용문 (동덕여자대학교 약학대학)
  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

As previously reported, an immunoglobulin M (IgM) monoclonal antibody (MAb) B6.1, specific for a cell wall B-l,2-mannotriose, was protective against vaginal infection due to Candida albicans when mice were treated with the antibody. In this study, the role of neutrophil was examined in the protective effect of MAb B6.1 against vaginal infection. To deplete neutrophils, mice were given intravenously rat anti-mouse neutrophile MAb RB6-8C5 prior to intraperitoneal administration of MAb B6.1 to these mice. The mice were examined for antibody in their reproductive tract. By an ELISA, MAb B6.1 was found in the vaginal homogenates, but no antibody was detected in vaginal lavage materials. The neutropenia was induced by a single dose of the anti-neutrophil antibody, but lymphocytes were also partially depleted. The protective effect of MAb B6.1 was decreased when mice pretreated with MAb RB6-8C5 were given the anti-Candida antibody before challenge with C. albicans yeast cells intravaginally. These results show that neutrophils are involved in the MAb B6.1 protection against Candida vaginal infection.

Keywords

References

  1. N. Engl. Med. v.324 Invasive Candida infections: evolution of a fungal pathogen Edwards, J. E. https://doi.org/10.1056/NEJM199104113241511
  2. J. Hosp. Infect. v.11 The emergence of fungi as major hospital pathogens Bodey, G. P. https://doi.org/10.1016/0195-6701(88)90220-4
  3. Clin. Infect. Dis. v.18 Hepatosplenic yeast infection in patients with acute leukemia: a diagnostic problem Anttila, V. J.;Ruutu, P.;Bondestam, S.;Jansson, S. E.;Nordlling, S.;Farkkil'a, M.;Sivonen, A.;Casttren, M.;Ruutu, T. https://doi.org/10.1093/clinids/18.6.979
  4. Rev. Infect. Dis. v.11 Fungemia caused by Candida species and Torulopsis glabrata in the hospitalized patient: frequency, characteristics, and evaluation of factors influencing outcome Komshian, S. V.;Uwaydah, A. K.;Sobel, J. D.;Crane, L. R. https://doi.org/10.1093/clinids/11.3.379
  5. Am. J. Obstet. Gynecol. v.152 Epidemiology and pathogenesis of recurrnt vulvovaginal candidiasis Sobe4l, J. D. https://doi.org/10.1016/S0002-9378(85)80003-X
  6. Ann. N. Y. Acad. Sci. v.544 Pathogenesis and epidemiology of vulvovaginal candidiasis Sobel, J. D. https://doi.org/10.1111/j.1749-6632.1988.tb40450.x
  7. Infect. Immun. v.63 Mice immunized by primary vaginal Candida albicans infection develop acquired vaginal mucosal immunity Fidel, P. L.;Lynch, M. E.;Conaway, D. H.;Tait, L.;Sobel, J. D.
  8. Infect. Immun. v.61 Candida-specific cell mediated immunity is demonstrable in mice with experimental vaginal candidiasis Fidel, P. L.;Lynch, M. E.;Sobel, J. D.
  9. Infect. Immun. v.63 Rats clearing a vaginal infection by Candida albicans acquire specific, antibody-mediated resistance to vaginal reinfection Cassone, A.;Boccanera, M.;Adriani, D.;Santoni, G.;De Bernardis, F.
  10. Infect. Immun. v.63 Antibody response that protects against disseminated candidiasis Han, Y.;Cutler, J. E.
  11. J. Infect. Dis. v.179 Candida albicans mannan extract-protein conjugates induce a protective immune response against experimental candidiasis Han, Y.;Ulrich, M.;Cutler, J. E. https://doi.org/10.1086/314779
  12. Infect. Immun. v.66 A vaccine and monoclonal antibodies that enhance mouse resistance to candida albicans vaginal infection Han, Y.;Morrison, R. P.;Cutler, J. E.
  13. Infect. Immun. v.65 A monoclonal antibody to Candida albicans enhances mouse neutrophil candidacidal activity Caesar-Ton That, T. C.;Cutler, J. E.
  14. J. Immunol. v.167 Complement is essential for protection by an IgM and an IgG3 monoclonal antibody against experimental disseminated candidiasis Han, Y.;Kozel, T. R.;Zhang, M. X.;MacGill, R. S.;Carroll, M. C.;Cutler, J. E. https://doi.org/10.4049/jimmunol.167.3.1550
  15. J. Immunol. v.152 Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection Czuprynski, C. J.;Brown, J. F.;Matoushek, N.;Wagner, R. D.;Steinberg, H.
  16. J. Exp. Med. v.181 Inhibition of tumor grown by elimination of granulocytes Pekara, L. A.;starr, B. A.;Toledano, A. Y.;Schreiber H. https://doi.org/10.1084/jem.181.1.435
  17. Infect. Immun. v.65 Biochemical characterization of Candida albicans epitopes that can elicit protective and nonprotective antibodies Han, Y.;Kanbe, T. K.;Cherniak, R.;Cutler, J. E.
  18. J. Infect. Dis. v.175 Assessment of a mouse model of neutropenia and the effect of an anti-candidiasis monoclonal antibody in these animals Han. Y.;Cutler, J. E. https://doi.org/10.1086/516455
  19. Infect. Immun. v.61 Binding of Candida albicans yeast cells to mouse popliteal lymph node tissue is mediated by macrophages Han, Y.;van Rooijen, N.;Cutler, J. E.
  20. Immunol. Today v.19 no.4 The molecular basis of leukocytosis Opdenakker, G.;Fibbe, W. E.;Damme, J. V. https://doi.org/10.1016/S0167-5699(97)01243-7