KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Identification of Endogenous Gibberellins by Feeding of [$^{14}\textrm{C}$] $\textrm{GA}_{12}$ in Chinese Yam, Dioscorea opposite

  • Lee, In-Jung (College of Agricultural Life Sciences, Division of Plant Biosciences, Kyungpook National University) ;
  • Kim, Sang-Kuk (Institute for Bioresources Research, Kyongbuk Provincial Agricultural Technology Administration) ;
  • Lee, Sang-Chul (College of Agricultural Life Sciences, Division of Plant Biosciences, Kyungpook National University) ;
  • Lee, Bong-Ho (Institute for Bioresources Research, Kyongbuk Provincial Agricultural Technology Administration) ;
  • Jang, Soo-Won (College of Agricultural Life Sciences, Division of Plant Biosciences, Kyungpook National University) ;
  • Park, Tae-Shik (Division of Rice Physiology and Cultivation, National Crop Experiment Station, Rural Development Administration)
  • Published : 2003.06.01
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Abstract

The metabolism of [$^{14}\textrm{C}$] $\textrm{GA}_{12}$ in the Chinese yam (Dioscorea opposita Thunb. var. Tsukune) was examined to determine the identification of endogenous gibberellins. [$^{14}\textrm{C}$] $\textrm{GA}_{12}$ was metabolized to $\textrm{GA}_{53}$, $\textrm{GA}_{44}$, $\textrm{GA}_{19}$, $\textrm{GA}_{20}$, $\textrm{GA}_1$, $\textrm{GA}_8$, $\textrm{GA}_{15}$, $\textrm{GA}_{24}$, $\textrm{GA}_9$, $\textrm{GA}_{36}$ and $\textrm{GA}_4$. Radioactivity of GAs in non C-13 hydroxylation route was five-fold higher than that of early C-13 hydroxylation in analyzed GA-metabolites. Radioactivity of $\textrm{GA}_4$ was always four times higher than that of $\textrm{GA}_1$ at every feeding time. $\textrm{GA}_1$ radioactivity has always a lower level to below 200 DPM. The major pathway of endogenous GA metabolism in seedlings of the Chinese yam might be the non C-13 hydroxylation pathway.

Keywords

References

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