대한한의학회지 (The Journal of Korean Medicine)

  • 제24권3호
  • /
  • Pages.96-107
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  • 2003
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  • 1010-0695(pISSN)
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  • 2288-3339(eISSN)
대한한의학회 (The Society of Korean Medicine)
권호 표지

평간개울지혈탕이 독성약물에 의한 간조직 손상에 미치는 영향

Effect of Pyunggangaeuljihyul-tang (Pinggankaiyuzhixue-tang) on Toxic Agent Induced Liver Cell Damage

  • 오세광 (동의대학교 한의과대학 비계내과학교실) ;
  • 김원일 (동의대학교 한의과대학 비계내과학교실) ;
  • 김우환 (동의대학교 한의과대학 비계내과학교실)
  • 발행 : 2003.09.01
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초록

Objective : This study was undertaken to determine if Pyunggangaeuljihyul-tang (Pinggankaiyuzhixue-tang, PG) has a protective effect against cell injury induced by various toxic agents in rabbit liver, Methods : Cell injury in vitro was estimated by measuring lactate dehydrogenase (LDH), and that in vivo was estimated by measuring alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity in serum. Lipid peroxidation was examined by measuring malondialdehyde, a product of lipid per oxidation. Results : PG prevented the LDH release by $CCl_4$, mercury, menadione, and tert-butyl hydroperoxide treatment in vitro in liver slices. The extent of protection by 2% PG was similar to that of $10{\mu\textrm{M}}$ N,N'-diphenyl-p-phenylenedianline, a potent antioxidant, in tert-butyl hydroperoxide-induced LDH release. PG also prevented lipid peroxidation and depletion of cellular ATP induced by Hg. Hg causes motphological changes including cell necrosis and its effect was significantly prevented by PG. When rats were treated intraperitoneatly with 0.5 ml/kg of $CCl_4$, serum alanine aminotransferase and aspartate aminotransferase activities were increased compared with the control, which was significantly inhibited by pretreatment of PG. PG also prevented reduction in GSH and lipid peroxidation induced by $CCl_4$ Conclusion : These results suggest that PG exerts aprotective effect against various toxic agents by its antioxidant action in liver tissues. Thus, PG may be used in prevention and treatment of drug-induced liver cell injury. However, the precise mechanisms of PG protection remain to be determined.

키워드

참고문헌

  1. 傅靑主女科 葉天士
  2. 방제학 貴陽中醫學院(主編)
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  4. 동의대학교 대학원 학위논문 平肝開鬱止血湯이 Oxidant에 의한 간조직 지질의 과산화에 미치는 영향 허재영
  5. 동의대학교 대학원 학위논문 平肝開鬱止血湯이 간조직에서 반응성 산소기 제거작용에 미치는 영향 김종국
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