Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Effects of Temperature and Urea on in vitro Aggregation of Tryptophan Synthase $\alpha$-Subunits

  • Park, Myung-Won (Department of Molecular Biology, College of Natural Sciences, Pusan National University) ;
  • Jeong, Jae-Kap (Department of Molecular Biology, College of Natural Sciences, Pusan National University) ;
  • Shin, Hae-Ja (Environmental Engineering Major, Division of Applied Engineering, Dongseo University) ;
  • Lim, Woon-Ki (Department of Molecular Biology, College of Natural Sciences, Pusan National University)
  • Published : 2003.12.01
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Abstract

Protein aggregation could be problematic as causes of diseases and hindrance in the production of useful recombinant proteins. Aggregation of mutant tryptophan synthase $\alpha$-subunits was examined by treatment with urea and at high temperature. Large amorphous aggregate seemed to appear by heat treatment, while more various aggregates in size were formed by treatment with urea at low concentration. The result indicates that different aggregate in size could be formed depending on the treatment condition, suggesting different mechanisms underlying aggregation processes.

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