퇴비로부터 분리된 Burkholderia cepacia No.15-2의 특성과 항균 효과

Characteristics and Antimicrobial Effects of Novel Burkholderia cepacia No. 15-2 Isolated from Compost

  • Yun, Soon-Il (Faculty of Applied Biological Science Hiroshima University)
  • 발행 : 2003.12.01

초록

길항미생물을 이용한 항곰팡이성 퇴비의 개발을 목적으로 공시 식물로써 Spinacia oleracea L과 식물 병원균 Rhizoctonia solani Kuhn O-28을 모델로 사용하여 수행 되었다. 다양한 음식쓰레기를 일년동안 숙성시킨 퇴비로부터 80 균주를 분리하였고, 그 중 No.15-2 균주가 R. solani Kuhn O-28에 대해 가장 높은 항곰팡이 활성을 보였다. 16S rDNA sequencing과 primer pair PCR에 의해 표현형과 분류학적으로 거의 독성이 없는 것으로 밝혀진 Burkholderia cepacia genomoval V로 분류되었다. 이 B. cepacia No. 15-2가 퇴비화 중에 우점하였으며 그 균수는 15일 동안 거의 $10^{13}$ cfu/g을 유지하였다. S. oleracea L을 배양 했을 경우 R. solani Kuhn O-28에 의한 발병율은 B. cepacia No.15-2를 첨가함으로써 40% 감소하였다. 결론적으로 B. cepacia No.15-2는 다양한 식물의 곰팡이 유래의 병을 방제하는 데 이용 가능할 것으로 사료된다.

To develop the functional-compost containing antifungal substance by using antagonistic microorganisms, Spinacia oleracea L and Rhizoctonia solani Kuhn O-28 were used as a model plant and phytopathogen, respectively. Total 80 strains were isolated from the compost of various waste foods mixture processed for a year. Among them, No.15-2 strain was selected due to its highest antifungal activity against R. solani Kuhn O-28 and was identified phyno- and phylogenotypically as Burkholderia cepacia genomovar V. which is rare probability in pathogen, by 16S rDNA sequencing and specific primer pair PCR method. B. cepacia No.15-2 preferentially dominated during the compost and its cell numbers were maintained almost $${\times}$10^{13}$ cuf/g for 15 days. The morbidity caused by R. solani Kuhn O-28 in S. oleracea L cultivation was reduced to 40% by addition of B. cepacia No.15-2. In conclusion, the antifungal compost using B. cepacia No.15-2 could be applied to biocontrol of various crops blights caused by fungal pathogen.

키워드

참고문헌

  1. Phytopathology v.14 Sour skin, a bacterial rot of onion bulbs Burkholder,W.H.
  2. Appl. Environ. Microbiol. v.66 Identification and characteristics of a novel Burkholderia strain with broad-spectrum antimicribioal activity Cain,C.C.;A.T.Henry;R.H.Waldo Ⅲ;L.J.Casida JR;J.O.Falkinham Ⅲ https://doi.org/10.1128/AEM.66.9.4139-4141.2000
  3. Int. J. Syst. Bacteriol v.45 Polyphasic taxonomy in the genus Burkholderia leading to an amended description of the genus and proposition of Burkholderia vietanmiensis sp. nov. for N₂-fixing isolates for rice in Vietnam Gillis,M.;T.V.Van;R.Bardin;M.Goor;P.Hebbar;A.Willems;P.Segers;K.Kersters;T.Heulin;M.P.Fernandez https://doi.org/10.1099/00207713-45-2-274
  4. Phytopathol. v.78 Biological control of blue mold and gray mold on apple and pear with Pseudomonas cepacia Janisiewicz,W.;J.Roitman
  5. Tetrahedron Letters v.37 Structural identification of cepaciamide A, a novel fungi-toxic compound form Pseudomonas cepacia D-202 Jiao,Y.;T.Yoshihara;S.Ishikuri;H.Uchino;A.Ichihara https://doi.org/10.1016/0040-4039(95)02342-9
  6. J. Clin. Microbiol. v.37 Development of rRNA-Based PCR assay for identification of Bukholdria cepacia complex isolates recovered from Cystic Fibrosis patients John,J.L.;J.D.Betty;D.M.Jenifer;W.W.Paul;L.S.Terrence;C.Tom;V.Peter
  7. Clin. Microbiol. Infect v.8 Differential invasion of respiratory epithelial cells by members of the Burkholderia cepacia complex Keig,P.M.;E.Inghant;P.A.R.Vandamme;K.G.Kerr https://doi.org/10.1046/j.1469-0691.2002.00356.x
  8. J. Microbiol. Biotechnol. v.9 Immunosuppressive activity of cepacidine A, a novel antifungal antibiotic produced by Pseudomonas cepacia Lee,C.H.;J.W.Suh;Y.H.Cho
  9. J. Antibiol v.47 Cepacidine A, a novel antifungal antibiotic produced by Pseudomonas cepacia.Ⅰ.Taxonomy, production, isolation and biological activity Lee,G.H.;S.Kim;B.Hyun;J.W.Suh https://doi.org/10.7164/antibiotics.47.1402
  10. Current Opinion in Pulmonary Medical v.4 Burkholderia cepacia epidemiology and pathogenesis:implication fro infection control Lipuma,J.J https://doi.org/10.1097/00063198-199811000-00005
  11. J. Clin. Microbiol v.37 Development of rRNA-Based PCR assay for identification of Burkholderia cepacia complex isolates recovered from cystic fibrosis patients Lipuma,J.J.;B.J.Dulaney;J.D.McMenamin;P.W.Whitby;T.L.Stull;T.Coenye;Vandamme,P.
  12. Pediatric Research v.35 PCR-based detection and typing of Pseudomonas cepacia, abstract. 2342 Lipuma,J.J.;G.J.Heidekcer;T.L.Stull
  13. Soft-science Co. New biological chemical science Matsunaka,S.
  14. J. Antibiot. v.21 Xylocandun;a new complex of antifungal peptide. Ⅰ. Taxonomy, isolation and biological activity Meyer,E.;G.S.Bisacchi;L.Dean
  15. J. Clin Microbiol v.38 Identification of members of Burkholderia cepacia complex by species-specific PCR Paul,W.W.;B.C.Karen;L.H.Kenneth;J.L.John;L.S.Terrence
  16. J. Clin. Microbiol. v.36 Comparison of culture and PCR for detection of Bukholderia cepacia in sputum samples of patients with cystic fibrosis Paul,W.W.;L.N.D.Hani;W.C.Preston;E.D.Tullis;M.Anne;L.S.Terrence
  17. J. Clin. Microbiol v.38 Identification and population structure of Burkholderia stabilis sp. nov. Peter,V.;E.Mahenthiralingam;B.Holmes;T.Coenye;B.Hoste;P.VosDe;D.Henry;P.D.Speert
  18. Endospore-forming Gram-positive rods and cocci v.1 Bergeys Manual of Systematic Bacteriology Sneath,P.H.A.;N.S.Mair;M.E.Sharpe;J.G.Holt
  19. Respirology v.7 Effect of Burkholderia cepacia infection in the clinical course of patients with cystic fibrosis: A pilot study in a Sydney clinic Soni,R.;G.Marks;D.A.Henry;M.Robinson;C.Moriarty;S.Parsons;O.Taylor;E.Mahenthiralingam;D.P.Speert;P.T.Bye https://doi.org/10.1046/j.1440-1843.2002.00387.x
  20. Int. J. Syst. Bacteriol v.47 Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov. Vandamme,P.;B.Holmes;M.Vancanneyt;T.Coenye;B.Hoste;R.Coop-man;H.Revets;S.Lauwers;M.Gillis;K.Kersters;J.R.Govan https://doi.org/10.1099/00207713-47-4-1188
  21. J. Clin. Microbiol v.36 Comparison of culture and RCR for detection of Burkholderia cepacia in sputum samples of patients with Cystic Fibrosis Whitby,P.W.;H.D.Dick;P.W.Campbell Ⅲ;D.E.Tullis;A.Matlow;T.L.Stull
  22. J. Clin. Microbiol. v.38 Identification of memvers of theBurkholderia sepacia complex by species-specific PCR Whitby,P.W.;K.B.Carter;K.L.Hatter;J.J.Lipuma;T.L.Stull
  23. Microbiol. Immunol. v.36 Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group Ⅱ to the new genus, with the type species Burkholderia cepacia(Palleroni and Holmes 1981) comb. nov. Yabuuchi,E.;Y.Kosako;H.Oyaizu;I.Yano;H.Hotta;Y.Hashimoto;T.Esaki;M.Arakawa