Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Immunostimulating and Anti-cancer Effects of Pediococcus pentosaceus EROM101 Isolated from Korea.

한국인으로부터 분리한 Pediococcus pentosaceus EROM101의 면역증강 및 항암활성

  • 송미경 ((주)이롬라이프 생명과학연구원) ;
  • 우석규 ((주)이롬라이프 생명과학연구원) ;
  • 장정순 ((주)이롬라이프 생명과학연구원) ;
  • 김중학 ((주)이롬라이프 생명과학연구원) ;
  • 김화영 ((주)이롬라이프 생명과학연구원) ;
  • 홍성길 ((주)이롬라이프 생명과학연구원) ;
  • 이병욱 ((주)이롬라이프 생명과학연구원) ;
  • 박미현 ((주)이롬라이프 생명과학연구원) ;
  • 정건섭 (연세대학교 생물자원공학과)
  • Published : 2003.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Immunostimulating effects of lactic acid bacteria as biological response modifier is a subject of growing interest, but the knowledge of these focused on some bacteria as Lactobacillus and Bifidobacterium. In this study, we investigated the effects of Pediococcus pentosaceus EROM101 on the immunostimulating and anti-cancer activity in murine model. P. pentosaceus was mainly found in Kimchi and fermented sea food and is facultatively anaerobic, catalase-netative, gram-positive cocci arranged in pairs, tetrads and clusters. The immunostimulating effects of P. pentosaceus EROM101 were evaluated using IgA production assay of Peyer's patch and proliferation assay of exudated immune cells of Balb/C mice fed P. pentosaceus EROM101 for 3 weeks. The macrophage and splenocyte proliferation were enhanced by orally administrated of P. pentosaceus EROM101. Also, IgA production in Peyer's patch increased by P. pentosaceus EROM101. Anti-cancer activity of P. pentosaceus EROM101 was appeared in Sarcoma 180 tumor-bearing ICR mice. However, this bacterium lysate itself appeared to have noncytotoxic substance against Sarcoma 180 cell in vitro. These results suggested that P. pentosaceus EROM101 reinforce immune system and therefore was revealed to be anti-cancer activity in mice.

사람 분변으로부터 대식세포 활성 증강효능이 있는 유산균을 선별하여 이를 동정한 결과 P. pentosaceus 동정되었으며, 이 균주를 P. pentosaceus EROM101로 명명하였다. P. pentosaceus EROM101의 면역증강 및 항암활성을 알아보기 위하여 실험동물에게 3주간 생균 및 파쇄액을 경구 투여한 뒤 대식세포와 비장세포의 활성화를 측정하였으며, 동시에 소장점막상피세포에서 생산되는 IgA의 함량을 측정하였다. P. pentosaceus EROM101의 경구 투여는 대식세포 및 비장세포의 분열을 촉진하여 면역증강 기능이 있는 것으로 나타났다. 특히, 생균보다 파쇄액에서 활성이 더 뛰어난 것으로 나타나, P. pentosaceus EROM101은 장내에서 생균으로 서식하여 지속적으로 면역증강 기능을 나타내는 것이 아닌 세포 구성물에서 면역증강활성이 있는 것으로 사료되었다. 또한, 소장점막상피세포에서 IgA의 분비량에서도 대조군보다 약 4배이상의 IgA 생산량을 증가시켜 장관면역 활성화 기능이 있는 것으로 판단되었다. 또한 P. pentosaceus EROM101 파쇄액이 Sarcoma 180 복수암세포를 이식해 준 실험동물 내에서 복수암성장을 억제하였으나, Sarcoma 180 세포열에 대한 세포독성을 나타내지 않아 면역증강에 의해서 항암 활성이 나타난 것으로 추측되었다. 이상의 연구결과에서 P. pentosaceus EROM101은 면역증강 능력이 있으며, 동시에 항암 활성을 기대할 수 있을 것으로 사료된다.

Keywords

References

  1. FEBS Lett v.1 Antitumour glycopeptides from Lactobacillus bulgaricus cell wall Bogdanov,I.G.;P.G.Dalev;A.I.Gurevich;M.N.Kolosov;V.P.Malekova;L.A.Plemyannikova;I.B.Sorokina https://doi.org/10.1016/0014-5793(68)80078-X
  2. Tohoku. J. Exp. Med v.125 Antitumor activity of hotwater extract from delipidated BCG Sato,H.;A.Yokosawa;H.Arai;H.Nagai;N.Kumano;M.Motomiya;K.Konno https://doi.org/10.1620/tjem.125.247
  3. J. Natl. Cancer. Inst. v.54 Nonspecific Immunotherapy of Murine Solid Tumors With Corynebacterium granulosum Milas,L.;B.Hunter;K.Mason;D.Grdina;H.Withers
  4. J. Immunol. 2001 v.166 Immunological and biochemical characterization of streptococcal pyrogenic exotoxins I and J (SPE-I and SPE-J) from Streptococcus pyogenes Proft,T.;V.L.Arcus;V.Handley;E.N.Baker;J.D.Fraser
  5. Clin. Microbiol. Rev. v.8 Identification, classification and clinical relevance of catalase-negative, gram-positive cocci, excluding the Streptococci and Enterococci Facklam,R.;J.A.Elliott
  6. J. Basic. Microbiol v.40 Analysis of the genetic determinant for production of the pediocin P of Pediococcus pentosaceus Pep1 Osmanagaoglu,O.;Y.Beyatli;U.Gunduz;S.C.Sacilik https://doi.org/10.1002/1521-4028(200008)40:4<233::AID-JOBM233>3.0.CO;2-H
  7. Chem. Pharm. Bull v.44 Chemical composition of the cell wall of lactic acid bacteria and related species de Ambrosini,V.M.;S.Gonzalez;G.Perdigon;H.A.P.de Ruiz;G.Oliver https://doi.org/10.1248/cpb.44.2263
  8. Korean. J. Biotechnol. Bioeng. v.13 Immunostimulating effects of mice fed with cell lysate of Lactobacillus plantrarum isolated from kimchi Chae,O.W.;K.S.Shin;H.W.Chung;T.B.Choe
  9. Ph.D. thesis. Chung-Ang Univ. Function and phyiological characteristics of lactic acid bacteria isolated from kimchi Park,I.S.
  10. Cancer. Immunol. Immunother v.20 Anti-tumour effect of humoral and cellular immunities mediated by a bacterial immunopotentiator, Lactobacillus casei, in mice Yasutake,N.;M.Ohwaki;M.Mutai;Y.Koide;T.Yoshida
  11. Cancer. Immunol. Immunother v.24 Antimetastatic effect of Lactobacillus casei YIT9018(LC9018) on a highly metastatic variant of B16 melanoma in C57BL/6J mice Matsuzaki,T.;T.Yokokura;I.Azuma
  12. Clin. Diag. Lab. Immunol v.6 Effects of orally administrated viable Lactobacillus rhamnosus GG and Propionibacterium freudenreichii subsp. sheramnii JS on mouse lymphocyte proliferation Kirjavainen,P.V.;H.S.Elnezami;S.J.Salminen;J.T.Ahokas;P.F.A.Wright
  13. J. Parenter. Enteral. Nutr v.23 Gut as the largest immunologic tissue Takahashi,I.;H.Kiyono https://doi.org/10.1177/014860719902300503
  14. Am. J. Clin. Nutr v.73 Probiotics:effects on immunity Isolauri,E.;Y.Sutas;P.Kankaanpaa;H.Arvilommi;S.Salminen
  15. Infect. Agents. Dis. v.2 New perspectives in vaccine development: mucosal immunity to imfections McGhee,J.R.;H.Kiyono
  16. Biosci. Biotechnol. Biochem v.62 Effects of orally ingested Bifidobacterium longum on the mucosal IgA response of mice to dietary antigens Takahashi,T.;N.T.Nakagawa;T.Yajima;T.Kuwata https://doi.org/10.1271/bbb.62.10
  17. J. Dairy. Sci. v.82 Ingestion of yogurt containing Lactobacillus acidophilus and Bifidobacterium to potentiates immunoglobulin A responses to cholera toxin in mice Tejada-Simon,M.V.;J..H.Lee;Z.Ustunol;J.JPestka https://doi.org/10.3168/jds.S0022-0302(99)75281-1
  18. Cancer Lett v.42 Antitumor activity of lactic acid bacteria on a solid fibrosarcoma, sarcoma-180 and Ehrlich ascites carcinoma Kelkar,S.M.;M.A.Shenoy;G.S.Kaklij https://doi.org/10.1016/0304-3835(88)90241-8
  19. Kor. J. Appl. Microbiol. Biotechnol. v.22 Antitumor activity of Bifidobacterium adolesnits ATCC-15703 against Sarcoma 180 in mice Kim,G.T.;H.Bae;Y.J.Baek;H.Y.Lee
  20. Biotherapy v.3 Antitumor action of an acidic glycoprotein(SAGP) from Streptococcus pyogenes in mice Yoshida,J.;S.Takamura;S.Suzuki https://doi.org/10.1007/BF02221325