Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Functional Analysis of Spectinomycin Biosynthetic Genes from Streptomyces spectabilis ATCC 27741

  • Jo, You-Young (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University) ;
  • Kim, Sun-Hee (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University) ;
  • Yang, Young-Yell (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University) ;
  • Kang, Choong-Min (Childrens Hospital, Harvard Medical School) ;
  • Sohng, Jae-Kyung (Department of Chemistry, SunMoon University) ;
  • Suh, Joo-Won (Department of Biological Science, Institute of Bioscience and Biotechnology, Myong Ji University)
  • Published : 2003.12.01
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Abstract

The function of genes related to spectinomycin biosynthesis (spcD, speA, speB, spcS2) from Streptomyces spectabilis ATCC 27741, a spectinomycin producer, was analyzed. Each gene was subcloned from a spectinomycin biosynthetic gene cluster and overexpressed in E. coli BL21 (DE3) using pET vector. After incubating each purified protein with its possible substrates, the final products were analyzed using high-performance liquid chromatography (HPLC). From these results, spcD, speA, and speB have been identified to be dTDP-glucose synthase, myo-inositol monophosphatase, and myo-inositol dehydrogenase, respectively. In addition, the results suggest that the spcS2 gene product functions downstream of the speB gene product in the biosynthetic pathway of spectinomycin. Taken together, the present study elucidates the early steps of the biosynthetic pathway for 6-deoxyhexose (6-DOH) part (actinospectose) and aminocyclitol part (actinamine) of spectinomycin.

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References

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