The Korean Journal of Ecology

한국생태학회 (The Ecological Society of Korea)
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16S rDNA염기서열에 의한 불가사리(Asterias amurensis) 장내에서 분리된 종속영양세균 군집의 다양성

The Diversity of Heterotrophic Bacteria Isolated from Intestine of Starfish(Asterias amurensis) by Analysis of 16S rDNA Sequence

  • 최강국 (충남대학교 자연과학대학 미생물학과) ;
  • 이오형 (목포대학교 자연과학대학 분자유적학) ;
  • 이건형 (군산대학교 자연과학대학 과학기술학부)
  • 발행 : 2003.12.01
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초록

본 연구는 2000년 7월에 전남 장흥군에서 채집한 불가사리의 장내에 존재하는 종속영양세균의 다양성에 대해서 알아보았다. 불가사리 장내에 존재하는 균체수를 측정하였으며, 순수 분리된 균주를 대상으로 16S rDNA 증폭기법을 이용하여 세균의 다양성을 조사하였다. 불가사리 장내에 분포하는 종속영양세균의 균체수는 8.65${\pm}$0.65${\times}10^3\;dfu\;g^{-1}$이었다. 29 균주의 세균이 순수 분리되었으며, 그 중 그람양성 세균은 분리된 균주의 59% (17균주)를 차지하였다. 불가사리 장내에서 분리된 균주는 Bacillus속, Microbacterium 속, 그리고 Marinobacter 속 등이 우점이었으며, 이외에도 Staphylococcus 속, Psychrobacter 속, Paracoccus 속, Erythrobacter 속, Zoogloea 속, Kocuria 속과 Arthrobacter 속 등이 포함되었다. 분리된 균주 가운데 Bacillus 속에 속하는 8균주 중 3균주는 type strain과 97% 이상의 유사도를 보인 반면, 5 균주는 유사도가 90%로 비교적 낮은 유사도를 보여 현재까지 알려지지 않은 신종일 가능성이 높다고 하겠다.

To study the diversity of heterotrophic bacteria isolated from intestine of starfish, Asterias amurensis, we collected starfishes from the coastal area near Jangheung-Gun, Jeollanam-Do, Korea during July, 2000. Population density and bacterial diversity in the intestine of starfish were measured. The results were as follows; The population densities of heterotrophic bacteria in the intestine of starfish were 8.65${\pm}$0.65${\times}10^3\;dfu\;g^{-1}$. Gram positive bacteria occupied 59% among 29 isolates. The community structure of dominant heterotrophic bacteria in the intestine of starfish consisted of Bacillaceae in the low G+C gram positive bacteria subphylum, Microbacteriaceae in the high G+C gram positive bacteria subphylum, and Alteromonadaceae in ${\gamma}$-Proteobacteria subphylum. Among eight strains of Bacillus spp., three strains showed more than 97% identity, but five strains showed about 90% identity with type strain on the basis of partial 16S rDNA sequence.

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