Abstract
The freeze dried young antler residue was extracted by proteases and hydrochloric acid(HCl). The young antler was extracted by water at 50$^{\circ}C$ and the residue was reacted by proteases for 5 hours at 50$^{\circ}C$. The extraction rate of its residue was 32.8%(absorbance 3.61 at 280nm) of bacteria protease, 23.8%(absorbance 0.69) of papain, and 31.2% (absorbance 2.96) of pepsin. The young antler was extracted by boiling water and the residue was reacted by proteases for 5 hours at 50$^{\circ}C$. The extraction rate of its residue was 45.0%(absorbance 3.61) of bacteria protease, 30.4%(absorbance 0.33) of papain, and 51.2% (absorbance 2.77) of pepsin. The result of HPLC analysis reveals that in 50$^{\circ}C$ water extract and boiling water extract, all high molecular peak was reduced under MW 1,000 by proteases. The result from the extract of young antler residue reacted by HCl for 5 hours at 50$^{\circ}C$ shows that its extraction rate was 45% (absorbance 0.78) in concentration of 0.1N HCl, 61% (absorbance 1.82) in 0.2N, 81% (absorbance 2.29) in 0.4N, and 82.0% (absorbance 3.28) in 2.0N. The result of HPLC analysis also reveals that in the extract by 0.8N HCl, the peak of about MW 70,000 accounted for 78% in total. Protein content of the extract by 0.8N HCl was 8.2%, and content of amino acid was 81.6%, ash was 1.3%, and mineral contents were 0.1 % of Ca, 2.3% of P, 0.8 % of Mg, 3.4% of Na, 0.002% of F by dry base.
녹용각질을 protease와 염산으로 가용화시킬 수 있는 최적 조건을 조사하였다. 녹용을 5$0^{\circ}C$에서 물로 추출하고 난 각질에 세균 protease를 가하여 5$0^{\circ}C$에서 5시간 추출한 것은 32.8%(흡광도 3.61), 파파인 추출액은 23.8%(흡광도 0.94), 펜신 추출액은 31.2%(흡광도 2.96)가 녹았다. 녹용을 끓여서 추출하고 남은 각질에 세균 protease를 가하여 5$0^{\circ}C$에서 5시간 반응시킨 것은 45.0%(흡광도 3.61), 파파인 추출액은 30.4%(흡광도 0.33), 펜신 추출액은 51.2%(홉광도 2.77)가 녹았다. HPLC로 분석한 결과, 저온 물추출 각질과 열수 추출각질은 protease에 의하여 모두 분자량 1,000 이하의 펩티드로 작아졌다. 녹용 각질에 염산을 가하여 5$0^{\circ}C$에서 5시간 반응시킨 결과, 염산 0.1N농도에서 45%(흡광도 0.78), 0.2N에서 61%(흡광도 1.82), 0.4N에서는 81.0%(흡광도 2.29), 2.0N에서 82.0%(흡광도 3.28) 녹았다. HPLC로 분석한 결과, 0.8N 염산으로 추출한 것은 분자량 7만 정도의 피크가 58%였다. 녹용을 0.8N 염산으로 추출하여 동결 건조한 분말의 단백질 함량은 8.2%, 아미노산 함량은 81.6%, 회분 함량은 1.3%를 나타냈고, 무기물 함량은 Ca 0.1%, P 2.3%, Mg 0.8%, Na 3,4%, F 0,02%를 나타냈다. 녹용각질 가용화의 최적조건은 세균 pretense를 작용시켜서 5$0^{\circ}C$에서 5시간 반응시킨 다음, 0.8N 염산으로 5시간 추출하는 것이었다.