Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Paeonia lactiflora Pall. Seed Extracts and Forsythia viridissima Lindl. Extracts on Antioxidative System and Lipid Peroxidation in Erythrocytes of Rats Fed High-Cholesterol Diet

  • Lee, Jeong-Min (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Rhee, Soon-Jae (Department of Food Science and Nutrition, Catholic University of Daegu)
  • Published : 2003.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the antioxidative effects of Paeonia lactiftora Pall. (PL) seed extracts and Forsythia viridissima Lindl. (FVL) extracts on the antioxidative defense system and lipid peroxidation in the erythrocytes of rats fed a high-cholesterol diet. Sprague-Dawley male rats weighing 100 10 g were randomly assigned to nine experimental groups and fed 0.5 % cholesterol. The HC group did not receive any supplement, while the MP group was supplemented with 0.1 % methanol extract of PL seed, the MP2 group with 0.2 % methanol extract of PL seed, the EP1 group with 0.05 % ether-soluble fraction of PL seed, the EP2 group with 0.1 % ether-soluble fraction of PL seed, the MS1 group with 0.05 % methanol extract of FVL, the MS2 group with 0.1 % methanol extract of FVL, the ES group with 0.025% ethyl acetate-soluble fraction of FVL, and the ES2 group with 0.05 % ethyl acetate-soluble fraction of FVL. The experimental diets were fed ad libitum for 3 weeks. The erythrocyte SOD activity in the EP1 and EP2 groups increased 38% and 59%, respectively, when compared with the HC group, while the erythrocyte GSHpx activity in the EP1, EP2, and ES2 groups increased 30%, 31 %, and 29%, respectively, when compared with the HC group. The level of erythrocyte TBARS was significantly lower in the MP2, EP1, and EP2 groups than in the HC group, yet the level of serum TBARS was significantly lower in the all supplemented groups than in the HC group. The level of serum HDL- TBARS was significantly lower in the EP1 and EP2 groups than in the HC group, while the level of serum LDL- TBARS was significantly lower in the all the supplemented groups than in the HC group. Accordingly, the results indicated that the PL seed extracts and FVL extracts reduced oxidative damage by activating the antioxidative defense system in the erythrocytes of rats fed a high-cholesterol diet.

Keywords

References

  1. Kor J Nut v.29 Nutritional probelms in Korea Moon SJ
  2. Kor J Nut v.29 Nutritional problems in Korean: Pattern of disease incidence and nutrition in korea Lee HG
  3. J Lipid Res v.21 Effect of saturated and polyunsaturated fat diet in the chemical composition and metabolism of low density lipoprotein in man Shepherd J;Packard CJ;Grundy SM;Yeshumin P;Gotto AM;Taunton OD
  4. Am J Cardiol v.90 The role of high-density lipoprotein(HDL) cholesterol in the prevention and treatment of coronary heart disease: expert group recommendation Sacks FM https://doi.org/10.1016/S0002-9149(02)02436-0
  5. Am J Clin Nutr v.17 Quantitative effects of dietary fat on serum cholesterol in man Hegsted DM;McGandy RB;Myers ML; Stare FJ
  6. Life Sci v.58 Effects of probucol on hyper cholesterolemia-induced changes in antioxidant enzymes Mantha SV;Kalra J;Prasad K https://doi.org/10.1016/0024-3205(95)02315-1
  7. Atherosclerosis v.81 Aortic antioxidant defence mechanisms: time-related changes in cholesterol-fed rabbits Del Boccio G;Lapenna D;Porreca E;Pennelli A;Savini F;Feliciani P;Ricci G;Cuccurullo F https://doi.org/10.1016/0021-9150(90)90019-F
  8. Biosci Biotechnol Biochem v.66 Improving effect of dietary taurine supplementation on the oxidative stress and lipid levels in the plasma, liver and aorta of rabbits fed on a high-cholesterol diet Balkan J;Kanbagli O;Hatipoglu A;Kucuk M;Cevikbas U;Aykac-Toker G;Uysal M https://doi.org/10.1271/bbb.66.1755
  9. J Atheroscler Thromb v.9 The protective effects of tetrahydrocurcumin on oxidative stress in cholesterol-fed rabbits Naito M;Wu X;Nomura H;Kodama M;Kato Y;Kato Y;Osawa T https://doi.org/10.5551/jat.9.243
  10. J Agric Food Chem v.50 Antioxidative activity of green tea polyphenol in cholesterol-fed rats Yokozawa T;Nakagawa T;Kitani K https://doi.org/10.1021/jf020029h
  11. Life Sci v.69 Antioxidative activity of naringin and lovastatin in high cholesterol-fed rabbits Jeon SM;Bok SH;Jang MK;Lee MK;Nam KT;Park YB;Rhee SJ;Choi MS https://doi.org/10.1016/S0024-3205(01)01363-7
  12. Int J Vitam Nutr Res v.72 Quercetin dihydrate and gallate supplements lower plasma and hepatic lipids and change activities of hepatic antioxidant enzymes in high cholesterol-fed rats Bok SH;Park SY;Park YB;Lee MK;Jeon SM;Jeong TS;Choi MS https://doi.org/10.1024/0300-9831.72.3.161
  13. Bonchohak Kang BS
  14. Korean resources plants Kim TJ
  15. Arch Pharm Res v.25 Cytotoxic and antimutagenic stilbents from seeds of Paeonia lactiflora Kim HJ;Chang EJ;Bae SJ;Shim SM;Park HD;Rhee CH;Park JH;Choi SW https://doi.org/10.1007/BF02976629
  16. Biosci Biotechnal Biochem v.66 Antioxidative activity of resveratrol and its derivatives isolated from seeds of Paeonia lactiflora Kim HJ;Chang EJ;Cho SH;Chung SK;Park HD;Choi SW https://doi.org/10.1271/bbb.66.1990
  17. Nutraceut Food v.7 Inhibition of tyrosinase and lipoxygenase activities by resveratrol and its derivatives from seeds of Paeonia lactiflora Kim HJ;Ha SC;Choi SW https://doi.org/10.3746/jfn.2002.7.4.447
  18. J Korea Acupuncture Moxibustion Soc v.16 Antioxidant effects of Paeoniae Radix aqua-acupuncture Lim CS;Kim KS
  19. J Korea Acupuncture Moxibustion Soc v.17 Effects of Paeoniae Radix aqua-acupuncture solution on tert-butyl hydroperoxide induced lipid peroxidation and antioxidative enzymes in cultured rat liver cells Moon JY
  20. Korea J Medicinal Crop Sci v.8 Screening of antioxidant and antimicrobial activity in native plants Rim YS;Park YM;Park MS;Kim KY;Kim MJ;Choi YH
  21. Korean J Food Sci Technol v.8 Antioxidative activity of ethanol extract from Korean medicinal plants Lim DK;Choi U;Shin DH
  22. Life Sci v.70 Antioxidant activity of anti-inflammatory plant extracts Schinella GR;Tournier HA;Prieto JM;Mordujovich D;Rios JL https://doi.org/10.1016/S0024-3205(01)01482-5
  23. Biol Pharm Bull v.23 Antiinflammatory effect of Forsythia suspensa V(AHL) and its active principle Ozaki Y;Rui J;Tang YT https://doi.org/10.1248/bpb.23.365
  24. Zhongguo Zhong Yao Za Zhi v.25 Investigation on antibacterial activity of Forsythia suspense Vahl in vitro with Mueller-Hinton agar Li ZX;Wang XH;Zhao JH;Yang JF;Wang X
  25. Kor J Pharmacogn v.19 Pharmacological studies on Forsythae fructus Lee EB;Keum HJ
  26. Korea J nutrition v.36 Effect of seed extract of Paeonia lactiflora on antioxidative system and lipid peroxidation of liver in rats fed high-cholesterol diet Lee JM;Choi SW;Cho SH;Rhee SJ
  27. Korean J Nutr v.36 no.10 Effect of Forsythia viridissima extracts on antioxidative system and lipid peroxidation of liver in rats fed high-cholesterol diet Lee JM;Choi SW;Cho SH;Rhee SJ
  28. Eur J Biochem v.47 Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase Marklund S;Mrklund G
  29. Biochem Biophys Res Commun v.71 Glutathione peroxidase activity in selenium-deficient rat liver Lawrence RA;Burk RF https://doi.org/10.1016/0006-291X(76)90747-6
  30. Biochem Med v.15 A simple fluorometric assay for lipoperoxide in blood plasma Yagi K https://doi.org/10.1016/0006-2944(76)90049-1
  31. Principles and procedures of statistics Yagi K
  32. Clin Biochem v.23 Rapid automated analysis of glutathione reductase, peroxidase and S-transferase activity: application to cisplatin-induced toxicity Bompart GJ;Prevot DS;Bascands JL https://doi.org/10.1016/0009-9120(90)80039-L
  33. Bioorg Med Chem v.10 Supplementation of naringenin and its synthetic derivative alters antioxidant enzyme activities of erythrocyte and liver in high cholesterol-fed rats Lee MK;Bok SH;Jeong TS;Moon SS;Lee SE;Park YB;Choi MS https://doi.org/10.1016/S0968-0896(02)00059-7
  34. Annu Rev Pharmacol Toxicol v.16 Chemicals, drugs, and lipid peroxidation Plaa GL;Witscri H https://doi.org/10.1146/annurev.pa.16.040176.001013