DOI QR코드

DOI QR Code

Induction of Quinone Reductase and Glutathione S-Transferase in Murine Hepatoma Cells by Flavonoid Glycosides

  • Kim, Jung-Hyun (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Lee, Jeong-Soon (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Kim, Young-Chan (Department of Food Science Technology, Kyungpook National University) ;
  • Chung, Shin-Kyo (Department of Food Science Technology, Kyungpook National University) ;
  • Kwon, Chong-Suk (Department of Food Science and Nutrition, Andong National University) ;
  • Kim, Young-Kyoon (Department of Forest Product, Kookmin University) ;
  • Kim, Jong-Sang (Department of Animal Science and Biotechnology, Kyungpook National University)
  • Published : 2003.12.01

Abstract

The potential of seven flavonoid glycosides to induce quinone reductase (QR), an anticarcinogenic marker enzyme, in murine hepatoma cells (hepalc1c7) and its mutant cells (BPRc1) was evaluated. Among test compounds, kaempferol-3-O-glucoside, luteolin-6-c-glucoside, and quercetin-3-O-glucoside (Q-3-G) induced QR in hepalc1c7 cells in a dose-dependent manner. However, in BPRc1 cells lacking arylhydrocarbon receptor nuclear translocator (ARNT), only Q-3-G caused a significant induction of quinone reductase at the concentration range of 0.5 to 8 ug/mL, suggesting that it is a monofunctional inducer. Q-3-G induced not only phase 2 enzymes, including QR and glutathione-S-transferase, but also nitroblue tetrazolium reduction activity in HL-60 cells, a biochemical marker for cell differentiation promoting agents. In conclusion, Q-3-G merits further study to evaluate its cancer chemopreventive potential.

Keywords

References

  1. Am J Med v.13 Bioactive compounds in foods: their role in the prevention of cardiovascular disease and cancer Kris-Etherton PM;Hecker KD;Bonanome A;Coval SM;Binkoski AE;Hilpert KF;Griel AG;Etherton TD
  2. Food Chem Toxicol v.37 Dietary flavonoids: Intake, health effects and bioavailability Hollman PCH;Katan MB https://doi.org/10.1016/S0278-6915(99)00079-4
  3. Food Chem Toxicol v.33 Review of the biology of quercetin and related bioflavonoids Formica JV;Regelson W https://doi.org/10.1016/0278-6915(95)00077-1
  4. Nutr Biochem v.7 Flavonoids-Chemistry, metabolism, cardioprotective effects, and dietary sources Cook NC;Saman S https://doi.org/10.1016/0955-2863(95)00168-9
  5. Carcinogenesis v.22 Total intracellular accumulation levels of dietary isothiocyanates determine their activity in elevation of cellular glutathione and induction of Phase 2 detoxification enzymes Ye L;Zhang Y https://doi.org/10.1093/carcin/22.12.1987
  6. Adv Enzyme Regul v.33 The electrophile counterattack response-Protection against neoplasia and toxicity Prestera T;Zhang YS;Spencer SR;Wilczak CA;Talalay P https://doi.org/10.1016/0065-2571(93)90024-8
  7. J Biol Chem v.265 Transcriptional regulation of the glutathione S-transferase Ya subunit gene-Characterization of a xenobiotic-responsive element controlling inducible expression by phenolic antioxidants Rushmore TH;Pickett CB
  8. Proc Natl Acad Sci USA v.85 Identification of a common chemical signal regulating the induction of enzymes that protect against chemical carcinogenesis Talalay P;Delong MJ;Prochaska HJ https://doi.org/10.1073/pnas.85.21.8261
  9. Adv Enzyme Regul v.28 Mechanisms of induction of enzymes that protect against chemical carcinogenesis Talalay P https://doi.org/10.1016/0065-2571(89)90074-5
  10. Carcinogenesis v.17 Dietary quercetin glycosides: Antioxidant activity and induction of the anticarcinogenic phase II marker enzyme quinone reductase in Hepalclc7 cells Williamson G;Plumb GW;Uda Y;Price KR;Rhodes MJC https://doi.org/10.1093/carcin/17.11.2385
  11. Carcinogenesis v.23 Dietary silymarin suppresses 4-nitroquinoline 1-oxide-induced tongue carcinogenesis in male F344 rats Yanaida Y;Kohno H;Yoshida K;Hirose Y;Yamada Y;Mori H;Tanaka T https://doi.org/10.1093/carcin/23.5.787
  12. Proc Natl Acad Sci USA v.77 Increase of NAD(P)H:quinone reductase by dietary antioxdants; Possible role in protection against carcinogenesis and toxicity Benson AM;Hukeler MJ;Talalay P https://doi.org/10.1073/pnas.77.9.5216
  13. J Biol Chem v.249 Glutathione Stransferases. The first enzymatic step in mercapturic acid formation Habig WH;Pabst MJ;Jakoby WB
  14. Methods Enzymol v.113 Determination of glutathione and glutathione disulfide in biological samples. Anderson ME https://doi.org/10.1016/S0076-6879(85)13073-9
  15. Anticancer Res v.15 Discovery of natural product chemopreventive agents utilizing HL-60 cell differentiation as a model Suh N;Luyengi L;Fong HH;Kinghorn AD;Pezzuto JM
  16. Proc natl Acad Sci USA v.89 A major inducer of anticarcinogenic protective enzymes from broccoli-Isolation and elucidation of Structure Zhng YS;Talalay P;Cho CG;Posner GH https://doi.org/10.1073/pnas.89.6.2399
  17. J Food Sci Nutr v.4 Induction of quionone reductase, an anticarcinogenic marker enzyme, by vitamin E in both hepa1c1c7 cells and mice Kwon CS;Kim J-S
  18. Toxicol Lett v.119 Induction of human NAD(P)H:quinone oxidoreductase (NQO1) gene expression by the flavonol quercetin Valerio LG Jr;Kepa JK;Pickwell GV;Quattrochi LC https://doi.org/10.1016/S0378-4274(00)00302-7
  19. J Nutr v.131 Curcumin and especially tetrahydrocurcumin ameliorate oxidative stress-induced renal injury in mice Okada K;Wangpoengtrakul C;Tanaka T;Toyokuni S;Uchida K;Osawa T
  20. Biochem Pharmacol v.60 Role of NAD(P)H:quinone oxidoreductase 1(DT diaphorase) in protection against quinone toxicity Joseph P;Long DJ 2nd;Klein-Szanto AJ;Jaiswal AK https://doi.org/10.1016/S0006-2952(00)00321-X
  21. Am J Clin Nutr v.62 Absorption of dietary quercetin glycosides and quercetin in healthy ileostomy volunteers Hollman PC;de Vries JH;van Leeuwen SD;Mengelers MJ;Katan MB
  22. Eur J Nutr v.42 Deglycosylation by small intestinal epithelial cell beta-glucosidases is a critical step in the absorption and metabolism of dietary flavonoid glycosides in humans Nemeth K;Plumb GW;Berrin JG;Juge N;Jacob R;Naim HY;Williamson G;Swallow DM;Kroon PA https://doi.org/10.1007/s00394-003-0397-3
  23. Biochem Pharmacol v.55 Transport of quercetin and its glucosides across human intestinal epithelial Caco-2 cells Walgren RA;Walle UK;Wall T https://doi.org/10.1016/S0006-2952(98)00048-3
  24. Biol Pharm Bull v.25 Differentiation-inducing effects of verticinone, an isosteroidal alkaloid isolated from the bulbus of Fritillaria ussuriensis, on human promyelocytic leukemia HL-6- cells Pae HO;Oh H;Choi BM;Oh GS;Paik SG;Jeong S;Hwang KM;Yun YG;Chung HT https://doi.org/10.1248/bpb.25.1409