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EFFECT OF DENTINAL TUBULES ORIENTATION ON PENETRATION PATTERN OF DENTIN ADHESIVES USING CONFOCAL LASER SCANNING MICROSCOPY

상아세관의 주행방향에 따른 상아질 접착제의 침투양상에 대한 공초점레이저주사현미경 연구

  • Kim, Dong-Jun (Dept. of Conservative Dentistry, College of Dentistry, DSRI, Chonnam Natl.Uni.) ;
  • Hwang, Yun-Chan (Dept. of Conservative Dentistry, College of Dentistry, DSRI, Chonnam Natl.Uni.) ;
  • Kim, Sun-Ho (Dept. of Conservative Dentistry, College of Dentistry, DSRI, Chonnam Natl.Uni.) ;
  • Oh, Won-Mann (Dept. of Conservative Dentistry, College of Dentistry, DSRI, Chonnam Natl.Uni.) ;
  • Hwang, In-Nam (Dept. of Conservative Dentistry, College of Dentistry, DSRI, Chonnam Natl.Uni.)
  • 김동준 (전남대학교 치과대학 보존학교실, 치의학연구소) ;
  • 황윤찬 (전남대학교 치과대학 보존학교실, 치의학연구소) ;
  • 김선호 (전남대학교 치과대학 보존학교실, 치의학연구소) ;
  • 오원만 (전남대학교 치과대학 보존학교실, 치의학연구소) ;
  • 황인남 (전남대학교 치과대학 보존학교실, 치의학연구소)
  • Published : 2003.09.01

Abstract

The purpose of this study was to evaluate the penetration pattern of dentin adhesives according to the orientation of dentinal tubules with confocal laser scanning microscopy. Specimens having perpendicular. parallel and oblique surface to dentinal tubules were fabricated. The primer of dentin adhesives (ALL $BOND^{\circledR}{\;}2,{\;}CLEARFIL^{TM}$ SE BOND and PQ1) was mixed with fluorescent material. rhodamine B isothio-cyanate (Aldrich Cherm. CO., Milw., USA), It was applied to the specimens according to the instructions of manufactures. The specimens were covered with composite resin (Estelite, shade A2) and then cut to a thickness of 500$\mu\textrm{m}$ with low speed saw (Isomet^{TM}, Buehler. USA). The adhesive pattern of dentin adhesives were observed by fluorescence image using confocal laser scanning microscopy. The results were as follows. 1. For the groups with tubules perpendicular to bonded surface. funnel shape of resin tag was observed in all specimen. However. resin tags were more prominent in phosphoric acid etching system (ALL $BOND^{\circledR}$ 2 and PQ1) than self etching system ($CLEARFIL^{TM}$ SE BOND). 2. For the groups with tubules parallel to bonded surface. rhodamine-labeled primer penetrated into peritubular dentin parallel to the orientation of dentinal tubules. But rhodamine-labeled primer of PQ1 diffused more radially into surrounding intertubular dentin than other dentin adhesive systems. 3. For the groups with tubules oblique to bonded surface. resin tags appeared irregular and discontinuous. But they penetrated deeper into dentinal tubules than other groups.

Keywords

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