Korean Journal of Materials Research (한국재료학회지)

Materials Research Society of Korea (한국재료학회)
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Fabrication of Au Nanoparticle for Au-conjugate Immuno Chemistry Probe

Au-conjugate 면역화학 진단용 금 나노입자 제조

  • Park, Sung-Tae (Department of Materials Science Engineering and Nano Technology Research Center Chonnam National University) ;
  • Lee, Kwang-Min (Department of Materials Science Engineering and Nano Technology Research Center Chonnam National University)
  • 박성태 (전남대학교 신소재공학부 및 나노기술연구센터) ;
  • 이광민 (전남대학교 신소재공학부 및 나노기술연구센터)
  • Published : 2003.08.01
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Abstract

Current nanogold cluster synthesized by chemical routine with 11 or 55 atoms of gold has been widely used for immuno chemistry probe as a form of nanocluster conjugated with biomolecules. It would be an undeveloped region that the 1 nm size of nanogold could be made by materials engineering processing. Therefore, objective of this study is to minimize the size of gold nanocluster as a function of operating temperature and chamber pressure in inert gas condensation (IGC) processing. Evaporation temperature was controlled by input current from 50 A to 65 A. Chamber pressure was controlled by argon gas with a range of 0.05 to 2 torr. The gold nanocluster by IGC was evaluated by X-ray diffraction (XRD) and transmission electron microscopy (TEM). The gold nanocluster for TEM analysis was directly sampled with special in-situ method during the processing. Atomic force microscopy (AFM) was used to observe 3-D nanogold layer surfaces on a slide glass for the following biomolecule conjugation step. The size of gold nanoclusters had a close relationship with the processing condition such as evaporation temperature and chamber pressure. The approximately 1 nm size of nanogold was obtained at the processing condition for 1 torr at $1124 ^{\circ}C$.

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