Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Photosynthetic Efficiency in Transgenic Tobacco Plants Expressing both CuZnSOD and APX in Chloroplasts against Oxidative Stress Caused by Highlight and Chilling

CuZnSOD와 APX를 엽록체에 발현시킨 담배식물체의 Highlight와 Chilling 스트레스에 대한 광합성 효율

  • Kim, Yun-Hee (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwon, Suk-Yoon (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Bang, Jae-Wook (Department of Biology, Chungnam National University) ;
  • Kwak, Sang-Soo (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 김윤희 (한국생명공학연구원 환경생명공학연구실) ;
  • 권석윤 (한국생명공학연구원 환경생명공학연구실) ;
  • 방재욱 (충남대학교 생물학과) ;
  • 곽상수 (한국생명공학연구원 환경생명공학연구실)
  • Published : 2003.12.01
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Abstract

In order to understand the protection effects of antioxidant enzymes against oxidative stress caused by various environmental stresses, transgenic tobacco (Nicotiana tabacum cv, Xanthi) plants expressing both copper/zinc superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) in chloroplasts (referred to as CA plants) were subjected to highlight (1,100$\mu$mol m$^{-2}$ sec$^{-1}$) and chilling at 4$^{\circ}C$. The protection effects of CA plants using leaf discs were compared with those of transgenic plants expressing either CuZnSOD or APX in chloroplasts (SOD plants or APX plants, respectively) and non-transgenic (NT) plants. CA plants showed about 15％ protection in the photosynthetic efficiency (Fv/Fm) of photosystem II relative to NT plants 1 hr after treatment of both highlight and chilling, whereas they showed about 23％ protection in the redox state of P700 in photosystem I at 3 hr after treatment. SOD plants or APX plants showed an intermediate protection effect between CA plants and NT plants. These results demonstrated that the coexpression of CuZnSOD and APX in chloroplasts importantly involves in the protection effects against oxidative stress caused by various environmental stresses.

환경스트레스에 대한 항산화효소의 보호효과를 이해하고 효과적인 스트레스내성 식물을 개발하기 위하여 CuZnSOD와 APX를 엽록체에 발현시킨 형질전환식물체 (CA식물체)에 강한 빛 (highlight, 1,100$\mu$mol m$^{-2}$ sec$^{-1}$)과 4$^{\circ}C$ chilling을 처리하였다. CA식물체 잎절편에서의 보호효과를 CuZnSOD을 도입한 SOD식물체, APX을 도입된 APX식물체 및 비형질전환식물체 (NT식물체)의 것과 비교하였다. CA식물체의 광계2에서의 광합성 효율 (Fv/Fm)은 highlight와 4$^{\circ}C$ chilling복합처리 1시간째에서 NT식물체에 비해 약 15%의 보호효과를 나타내었고, 광계1에서의 P700 redox state는 처리 후 3시간째에 약 23%의 보호효과를 나타내었다. SOD식물체와 APX식물체의 복합처리에 대한 보호효과는 CA식물체와 NT식물체의 중간 효과를 나타내었다. 이러한 결과는 엽록체에서 CuZnSOD와 APX의 동시발현이 highlight와 chilling에 의한 산화스트레스를 극복하는 데 매우 중요하게 관여함을 직접적으로 제시하는 것이다.

Keywords

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Cited by

  1. Transgenic Plants with Enhanced Tolerance to Environmental Stress by Metabolic Engineering of Antioxidative Mechanism in Chloroplasts vol.32, pp.3, 2005, https://doi.org/10.5010/JPB.2005.32.3.151
  2. Effects of Chilling on the Structure, Function and Development of Chloroplasts vol.9, pp.1664-462X, 2018, https://doi.org/10.3389/fpls.2018.01715