Enantioselective Hydrolysis of Racemic Styrene Oxide by Epoxide Hydrolase of Rhodosporidium kratochvilovae SYU-08

  • Lee, Ji-Won (Department of Food Science and Technology, Kyungsung University) ;
  • Lee, Eun-Jung (Department of Food Science and Technology, Kyungsung University) ;
  • Yoo, Seung-Sik (Department of Chemical Engineering, Pusan National University) ;
  • Park, Sung-Hoon (Department of Chemical Engineering, Pusan National University) ;
  • Kim, Hee-Sook (Department of Food Science and Technology, Kyungsung University) ;
  • Lee, Eun-Yeol (Department of Food Science and Technology, Kyungsung University)
  • 발행 : 2003.10.01

초록

Enantioselective hydrolysis for the production of chiral styrene oxide was investigated using the epoxide hydrolase activity of a newly isolated Rhodosporidium kratochvilovae SYU-08. The effects of reaction parameters - buffer type, pH, temperature, initial substrate concentrations, phenyl-1,2-ethanediol concentrations on hydrolysis rate, and enantioselectivity - were analyzed. Optically active (S)-styrene oxide with an enantiomeric excess higher than 99 % was obtained from its racemate. with a yield of 38 % (theoretically 50% maximum yield) from an initial concentration of 80 mM.

키워드

참고문헌

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