Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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High Frequency Bulblet Formation in Scale and Stem Thin Cell Layer Explant Cultures of Lilium Oriental Hybrids

오리엔탈 백합의 인편 및 줄기의 박판 세포층 절편으로부터 고빈도 자구형성

  • Oh, Seung-Cheol (Laboratory of Plant Genomics Services, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Chung, Myung-Hee (Laboratory of Plant Genomics Services, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Suk-Won (Laboratory of Plant Genomics Services, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Liu, Jang-Ryol (Laboratory of Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 오승철 (한국생명공학연구원 식물유전체연구소재은행실) ;
  • 정명희 (한국생명공학연구원 식물유전체연구소재은행실) ;
  • 김석원 (한국생명공학연구원 식물유전체연구소재은행실) ;
  • 유장렬 (한국생명공학연구원 식물유전체연구소재은행실, 식물세포공학연구실)
  • Published : 2003.09.01
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Abstract

An efficient system for in vitro bulblet formation of Lilium oriental hybrids(cvs). Casa Blanca and Siberia is described. Transverse thin cell layer(tTCL)(1mm thick) explants of 'Casa Blanca' formed bulblets at a frequency of 97.7％ when cultured on Murashige and Skoog (MS) medium supplemented with 1mg/L 2,4 dichlorophenoxyacetic acid(2,4-D) (On average 15.6 bulblets were formed per explant). The frequency of bulblet formation was drastically reduced when the explant ghickness was thinner than 1 mm. Explants from the outermost layer of bulb scale produced greater frequency of bulblet formation than middle or innermost layer. Among auxins supplemented to culture medium at 1 mg/L, 2,4-D led to greater frequency of bulblet formation on explants than dicamba, picamba, or phenylacetic acid(PAA). tTCL explants from the middle region of the outermost layer bulb scale yielded greater frequency of bulblet formation than the upper or lower region. tTCL stem explants of 'Siberia' formed bulblets at a frequency of 95.3% when cultured on MS medium with 1 mg/L 2,4-D(On average 9.1 bulblets were formed per explant). The system estabilished in this study will be useful for in vitro rapid propagation and genetic transformation of Lilium Oriental hybrids.

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References

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