Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Molecular Cloning and Characterization of a Peroxiredoxin cDNA from Cell Cultures of Sweetpotato

고구마 배양세포에서 Peroxiredoxin cDNA의 분리 및 발현 특성

  • Park, Soo-Young (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience Biotechnology (KRIBB)) ;
  • Ryu, Sun-Hwa (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience Biotechnology (KRIBB)) ;
  • Kwon, Suk-Yoon (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience Biotechnology (KRIBB)) ;
  • Kim, Jong-Guk (Department of Microbiology, Kyungpook National University) ;
  • Kwak, Sang-Soo (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience Biotechnology (KRIBB))
  • 박수영 (한국생명공학연구원 환경생명공학연구실) ;
  • 류선화 (한국생명공학연구원 환경생명공학연구실) ;
  • 권석윤 (한국생명공학연구원 환경생명공학연구실) ;
  • 김종국 (경북대학교 미생물학과) ;
  • 곽상수 (한국생명공학연구원 환경생명공학연구실)
  • Published : 2003.06.01
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Abstract

Peroxiredoxin(Pix) are large family of peroxidases that reduce alkyl hydroperoxides and hydrogen peroxide. A cDNA clone (referred to as swPrxl) encoding Pix was from a sweetpotato cDNA library constructed from suspension-sultured cells, and its expression was investigated in terms of stress. The swPrxl contained an open reading frame (ORF) encoding mature protein of 193 amino acids with calculated molecular mass of 20.8kDa. The predicted amino acid sequence of swPrxl has two conserved cysteines that are essential resicues for the reduction of peroxides. It showed high amino acid sequence homology ot PixIIF of Arabidopsis (77%) and putative Prx of rice(72%). RNA gel-blot analysis showed that swPrxl gene was expressed dominantly in leave among intact tissues, and also highly detect in suspension-cultured cells. Interestingly, the level of swPrxl transcripts was almost the same regardless of the growth stage in suspension culture. Furthermore, the transcription level of swPrxl gene was not significantly changed in response to various stress treatments such as wounding, extreme temperature and stress-related chemicals RT-PCR analyses.

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