Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
권호 표지
DOI QR코드

DOI QR Code

Changes of Vascular Contractility of isolated Rat Aorta treated with Salt Stress

Salt 스트레스에 의한 흰쥐 적출대동맥의 수축력 변화양상

  • 김종일 (경북대학교 의학과 약리학교실) ;
  • 박태규 (경북대학교 의학과 약리학교실) ;
  • 김중영 (경북대학교 의학과 약리학교실)
  • Published : 2003.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

To examine whether salt stress would alter or not contractility of isolated rat aorta, under anesthesia with sodium pentobarbital(50 mg kg-1 i.p.), male Sprague Dawley rats(300-330 g) were subjected to 0, 50, and 150 mM of sodium chloride at 37$^{\circ}C$ for 60 min. where as the sham group was left at modified Krebs-bicarbonate solution. To measure contractile response of vascular ring preparation isolated from rat was determined in organ bath and was recorded on physiograph connected to isometric transducer. And the strip was checked for expression of heat shock protein(Hsp) by Western blotting. One, three and eight hours later, we measured vascular contractility of isolated rat aorta treated with KCI, phenylephrine from organ bath study. The dose-vascular responses of potassium chloride and phenylephrine showed a little augmentation by NaCl concentration in the strips exposed to NaCl for 8 hours. And the response of relaxation induced by nitroprusside and acetylcholine was not influenced by NaCl stress in isolated aorta ring for 8 hours, respectively. Expression pattern of Hsp 70 of vascular muscle in isolated rat aorta showed a little increase in 150 mM NaCl group at 8 hours after NaCl treatment but not at 3 hours, and Hsp 60 expression of rat aorta was markedly increased in 50 mM NaCl group at 8 hours after NaCl treatment. Taken together, NaCl induced dose-and time dependent accumulation of the Hsp but not affected contraction of rat aorta. These data suggest that short term high salt stress was not sufficient to induce hypertension of rat aorta.

Keywords

References

  1. Muntzel, M. and T. Drueke, 1992, A comprehensive review of the salt and blood pressure relationship, Am. J. Hypertension, 5, 1S-42S https://doi.org/10.1093/ajh/5.4S.1S
  2. Wu, C. and C. H. Fly, 2001, $Na^+/Ca2^+$ exchange and its role in intracellular Cat regulation in guinea pig detrusor smooth muscle, Am. J. Physiol., 280, C1090-C1096 https://doi.org/10.1152/ajpcell.2001.280.5.C1090
  3. Caplea, A., D. Seachrist, G. Dunphy and D. Ely, 2001, Sodium-induced rise in blood pressure is suppressed by androgen receptor blockade, Heart and Circulatory Physiol., 280(4), 1793-1801 https://doi.org/10.1152/ajpheart.2001.280.4.H1793
  4. Henry, J. P., Y. Liu, W. Nadra, C. Quan, P. Mormede, V. Lemaire, D. Ely and E. Hendley, 1993, Psychosocial stress can induce chronic hypertension in normotensive strains of rats, Hypertension Soc. Sci. Med., 26, 293-302
  5. Egan, B. M., J. Petrin and R. G. Hoffmann, 1991, NaCl induces differential changes of regional vascular reactivity in salt-sensitive versus salt-resistant men, Am. J. Hypertens, 4(ptl), 924-931 https://doi.org/10.1093/ajh/4.12.924
  6. Fang, Z., S. H. Carlson, N. Peng and J. M. Wyss, 2000, Circadian rhythm of plasma sodium is disrupted in spontaneously hyper-tensive rats fed a high-NaCl diet, Regul. Integrative and Comparative Physiology, 278, 1490-1495 https://doi.org/10.1152/ajpregu.2000.278.6.R1490
  7. Kurokawa, K., 2001, Salt, kidney and hypertension: why and what to learm from genetic analyses?, Nephron, 89(4), 369-376 https://doi.org/10.1159/000046105
  8. Buemi, M., M. Senatore, F. Corica, C. Aloisi, A. Romeo, D. Tramontana and N. Frisina, 2002, Diet and arterial hypertension: is the sodium ion alone important?, Med. Res. Rev., 22(4), 419-428 https://doi.org/10.1002/med.10013
  9. Udelsman, R., M. J. Blake and N. J. Holbrook, 1991, Molecular response to surgical stress: specific and simultaneous heat shock protein induction in the adrenal cortex, aorta, and vena cava, Surgery, 110, 1125-1131
  10. Kim, Y. H., J. H. Kim, M. K. Kim, W. Y. Baek and I. K. Kim, 1999, Effect of heat shock on the vascular contractility in isolated rat aorta, J. Pharmacol. Toxicol. Methods, 42, 171-174 https://doi.org/10.1016/S1056-8719(00)00068-X
  11. Yang, E. K. and I. K. Kim, 2001, Pulse exposure to ethanol augments vascular contractility through stress response, Kor. J. Physiol. Phmacol., 5, 47-53
  12. Park, T. G., Y. J. Kwon and C. Y. Kim, 2003, Effects of arsenic on heat shock protein and vascular contractility of rat aorta, J. of the Environ. Sci., 12(6), 651-657
  13. Benjamin, I J. and D. R. McMillan, 1998, Stress (Heat Shock) Proteins, Circulation Research, 83, 117-132 https://doi.org/10.1161/01.RES.83.2.117
  14. Blake, M. J., L. M. Klevay, E. S. Halas and A. M. Bode, 1995, Blood pressure and heat shock protein expression in response to acute and chronic stress, Hypertension, 25, 539-540 https://doi.org/10.1161/01.HYP.25.4.539
  15. Cohen, D. M., J. C. Wasserman and S. R. Gullans, 1991, lmmediate early gene and Hsp70 expression in hyperosmotic stress in MDCK cells, Am. J Physiol., 261, C594-C601 https://doi.org/10.1152/ajpcell.1991.261.4.C594
  16. Hurwitz, L., 1986, Pharmacology of calcium channels and smooth muscle, Ann. Rev. Pharmacol., 26, 225-258 https://doi.org/10.1146/annurev.pa.26.040186.001301
  17. Khalil, R. A. and C. van Breemen, 1995, Mechanisms of calcium mobilization and homeostasis in vascular smooth muscle and their relevance to hypertension. In: Laraagh, J. H., and B. M. Brenner, eds. Hypertension: Pathophysiology, Diagnosis, and Management, New York, NY: Raven Press, 523-540pp
  18. Carlson, S. H., S. Oparil, Y. F. Chen and J. M. Wyss, 2002, Blood Pressure and NaCl-Sensitive Hypertension Are Influenced by Angiotensin-Converting Enzyme Gene Expression in Transgenic Mice, Hypertension, 39 (2), 214-221 https://doi.org/10.1161/hy0202.104267
  19. Ouchi, Y., L. Share, J. T. Crofton, K.Iitake and D. P. Brooks, 1998, Sex difference in pressor responsiveness to vasopressin and baroreflex function in DOC-salt hypertensive rats, J. Hypertens, 6, 381-387
  20. Brown, W. J., F. K. Brown and I. Krishan, 1971, Exchangeable sodium and blood volume in normotensive and hypertensive humans on high and low sodium intake, Circulation, 43, 508-519 https://doi.org/10.1161/01.CIR.43.4.508
  21. Kuller, L. H., 1997, Salt and blood pressure: population and individual perspectives, Am. J. Hypertension, 10(pt 2), 29S-36S https://doi.org/10.1016/S0895-7061(97)00071-X
  22. Lee, J. T., E. B. Lee, CR. Park, I. K. Kim, W. S. Yu and Y. S. Yoo, 1998, Reactivity of Human Isolated Gastroepiploic Artery to constrictor and Relaxant Agents, Kor. J. Thoracic and Cardiovascular Surgery, 31(9), 884-891
  23. Lindquist, S. and E. A. Craig, 1988, The heat shock proteins, Ann. Rev. Genet., 22, 631-677 https://doi.org/10.1146/annurev.ge.22.120188.003215
  24. Muller, E., W. Neuhofer, A. Ohno, S. Rucker, K. Thurau and F. X. Beck, 1996, Heat shock proteins Hsp25, Hsp60, Hsp72, Hsp73 in isoosmotic cortex and hyperosmotic medulla of rat kidney, Pflugers Arch., 431, 608-617 https://doi.org/10.1007/BF02191910