DOI QR코드

DOI QR Code

락토스 오페론에서 Cyclic AMP Receptor Protein에 의한 두 결합 부위(CRP1과 CRP2)의 결합 특성에 관한 연구

The Binding Affinities of Two Binding Sites(CRP1 and CRP2 Sites) by Cyclic AMP Receptor Protein at Lactose Operon

  • 강종백 (경원대학교 화학과) ;
  • 권건 (경원대학교 화학과)
  • Kang, Jong-Baek (Department of Chemistry, Kyungwon University) ;
  • Kwon, Gun (Department of Chemistry, Kyungwon University)
  • 발행 : 2003.10.01

초록

Lactose operon contains two CRP binding sites at promoter(CRP1 site) and operator(CRP2 site) regions at lac operon. CRP protein can bind to both sites with the different binding affinity. CRP1 site, major CRP binding site, acts the transcription activation with the fully unknown mechanism by binding of CRP. In this study, the binding affinities of CRP1 site and CRP2 site were measured with the fluorescein-labeled oligomers, which contain CRP1 site and the three different spacing sequences between GTGA and TCAC at CRP2 site. Results showed that CRP:cAMP complex bound to CRP1 site 3 times more strongly than CRP2 site and the base spacing between GTGA and TCAC was not the only factor to affect the binding affinity of CRP to CRP2 site.

키워드

참고문헌

  1. Nucleic Acids Res. v.21 Mutagenesis of the cyclic AMP receptor protein of Escherichia Coli: targeting positions of 72 and 82 of cyclic nucleotide binding pocket Belduz,A.O.;E.J.Lee;J.G.Harman https://doi.org/10.1093/nar/21.8.1827
  2. J. Biol. Chem. v.273 Interactive and dominant effects of residues 128 and 141 on cyclicnucleotide and DNA bindings in Escherichia coli cAMP receptor protein Cheng,X.;J.C.Lee https://doi.org/10.1074/jbc.273.2.705
  3. J. Mol. Biol. v.182 Analogs of cyclic AMP that elicit the biochemically defined conformational change in catabolite gene activator protein(CAP) but do not stimulate binding to DNA Ebright,R.H.;Le Grice,S.F.J.;Miller,J.P.;J.S.Krakow https://doi.org/10.1016/0022-2836(85)90030-0
  4. J. Biol. Chem. v.261 Structure-function analysis of three cAMP-dependent forms of the cAMP receptor protein Harman,J.G.;K.McKenney;A.Peterkofsky
  5. J. Mol. Biol. v.281 Relative binding affinities of OmpR and OmpR-phosphate at the ompF and ompC regulatory sites Head,C.G.;A.Tardy;L.J.Kenney https://doi.org/10.1006/jmbi.1998.1985
  6. Biochemistry v.28 Escherichia coli cAMP receptor protein evidence for three protein conformational states with different promoter binding affinities Heyduk,T.;J.C.Lee https://doi.org/10.1021/bi00443a021
  7. Proc. Natl. Acad. Sci. USA v.87 Application of fluorescence energy transfer and polarization to monitor Escherichia coli cAMP receptor protein and lac promoter interaction Heyduk,T.;J.C.Lee https://doi.org/10.1073/pnas.87.5.1744
  8. J. of Bacteriol. v.173 The binding of cyclic AMP receptor protein to two lactose promoter sites is not cooperative in vitro Hudson,J.M.;M.G.Fried
  9. Ann. Rev. Biochem. v.62 Transcriptional regulation by cAMP and its receptor protein Kolb,A.;H.Buc;S.Garges;S.Adhya https://doi.org/10.1146/annurev.bi.62.070193.003533
  10. Nucleic Acid Res. v.22 Mutagenesis of the cyclic AMP receptor protein of Escherichia Coli: targeting positions of 83, 127 and 128 of cyclic nucleotide binding pocket Lee,E.J.;J.Glasgow;S.F.Leu;A.O.Beluz;J.G.Harman https://doi.org/10.1093/nar/22.15.2894
  11. J. Biol. Chem. v.257 Structure of catabolite gene activator protein at 2.9 angstrom resolution Mckay,D.B.;I.T.Weber;T.A.Steitz
  12. FEBS Letters v.453 Functional roles of the cyclic AMP-dpendent forms of cyclic AMP receptor protein from Escherichia coli Mukhopadhyay,J.;R.Sur;P.Parrack https://doi.org/10.1016/S0014-5793(99)00719-X
  13. J. Bacteriol. v.174 Catabolite gene activator protein activation of lac transcription Reznikoff,W.S.
  14. Nucleic Acids Res. v.9 Cyclic AMP receptor protein interacts with lactose operator DNA Schmidt,A. https://doi.org/10.1093/nar/9.2.277
  15. Biochemistry v.39 Intersubunit association induces unique allosteric de pendence of the T127L CRP mutant on pH Shi,Y.;S.Wang;F.P.Schwarz https://doi.org/10.1021/bi000225m
  16. J. Mol. Biol. v.198 Structure of a complex of catabolite gene activator protein and cyclic AMP defined at 2.5 angstrom resolution Weber,I.T.;T.A.Steitz https://doi.org/10.1016/0022-2836(87)90315-9