Environmental Analysis Health and Toxicology

환경독성보건학회 (The Korean Society of Environmental Health and Toxicology)
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내분비계 장애물질인 Bisphenol A의 free radical 생성을 통한 독성발현

Cytotoxicity of Environmental Estrogenic Compound, Bisphenol A, via Generation of Free Radicals

  • 발행 : 2003.09.01
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초록

Bisphenol A shares similarities in structure, metabolism and action with DES, a known human teratogen and carcinogen. Bisphenol A, a monomer of polycarbonate and epoxy resins, has been detected in canned food and human saliva. The purpose of the this study was to evaluate the cytotoxicity, cell proliferation of bisphenol A In the presence of a rat liver S9 mix, contaning cytochrome P450 enzymes, and Cu (II). In the present study, Bisphenol A in combination with Cu (II) exhibited a enhancement in cytotoxicity which were inhibited by free radical scavengers. The content of malondialdehyde, an end product of lipid peroxidation, was also found to increase with concentration of bisphenol A. Also, we examined the change of CuZn-SOD, Mn-SOD, catalase and GPx activities in the MCF-7 cells exposed to bisphenol A. The activities of CuZn-SOD, CPx, catalase were found to decrease with bisphenol A concentration. Meanwhile, the activity of Mn-SOD was unchanged. This indicated that elevated oxidative stress caused by imbalance between the production and removal of free radicals occurred in cells.

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참고문헌

  1. 김현진, 김봉희. In vitro 실험법에 의한 천연물 중의 UVA 광독선 억제제 검색. 한국환경독성학회지 2002; 17(3): 253-259
  2. Arnold SF and McLachlan JA. Synergistic signals in the environment, Environ. Health. Perspect. 1996; 101: 1020-1023
  3. Birnbaum LS. Endocrine effects of prenatal exposure to PCBs, dioxins, and other xenobiotics: implications for policy and future research. Environ. Health. Perspect., 1994; 102: 676-679 https://doi.org/10.2307/3432197
  4. Bolt HM. Metabolism of estrogens-natural and synthetic, Pharm. Ther. 1979; 4: 155-181 https://doi.org/10.1016/0163-7258(79)90018-4
  5. Brotons JA, Olea-Serrano MF, Villalobos M, Pedraza V and Olea N. Xenoestrogens released from lacquer coatings in food cans. Environ health Perspect. 1995; 103: 608-612 https://doi.org/10.2307/3432439
  6. Bruder JM, Sobek L and Oettel M. Dehydroepiandroestrone stimulates the estrogen response element, J. Steroid. Biochem. Mol. Biol. 1997; 62: 461-466 https://doi.org/10.1016/S0960-0760(97)00056-3
  7. Cavalieri EL, Stack DE, Devanesan PD, Todorovic R, Dwivedy I, Higginbotham S, Johansson SL, Patil KD, Gross ML, Gooden JK, Ramanathan R, Cerny RL and Rogan EG. Molecular origin of cancer: catechol estrogen 3,4-quinones as endogenous tumors initiators, Proc. Natl. Acad. Sci. USA 94, 1997; 10937-10942 https://doi.org/10.1073/pnas.94.20.10937
  8. Colborn T, Dumanoski D and Myers JP. In our stolen Future: Are We Threatening Our Fertility, Intelligence, and Survival? A Scientific Detective Story. Dutton Books, New York. 1996
  9. Davis DL, Bredlow HL, Wolff M, Woodruff T, Hoel DG, and Anton-Culver H. Medical hypothesis: Xenoestrogens as preventable causes of breast cancer, Environ. Health Perspect. 1993; 101: 372-377 https://doi.org/10.2307/3431889
  10. Dhar JD, Mishra R and Setty BS. Estrogen, androgen and antiestrogen responses in the accessory organs of male rats during different phases of life, Endocr. Res. 1998; 24: 159-169 https://doi.org/10.1080/07435809809135525
  11. Kavlock RJ and Ankley GT. A perspective on the risk assement process for endocrine = disruptive effects of wildlife and human health, Risk Anal. 1996; 16: 731-739 https://doi.org/10.1111/j.1539-6924.1996.tb00824.x
  12. Roy D and Liehr JG. Estrogen, DNA damage and mutations, Mutat. Res. 1999; 424: 107-115 https://doi.org/10.1016/S0027-5107(99)00012-3
  13. Safe SH. Environmental and deitary estrogens and human health: Is there a problem? Environ. Health Perspect., 1995; 103: 346-351 https://doi.org/10.2307/3432287
  14. Seacat AM, Kuppusamy P, Zweier JL and Yager JD. ESR identification of free radicals formed from the oxidation of catechol estrogens by $Cu^{2+}$, Arch, Biochem. Biophys. 1997; 347: 45-52 https://doi.org/10.1006/abbi.1997.0323
  15. Sharpe RM and Skakkebake NE. Are oestrogens involved in failing sperm ounts and disorders of the male reproductive tract?, Lancet 1993; 341: 1392-1395 https://doi.org/10.1016/0140-6736(93)90953-E