Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
권호 표지

Isolation and Selection of Actinomycetes Producing Anti-fungal Materials

항진균성 활성물질을 생성하는 토양방선균의 분리

  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Anti-fungal materials producing bacteria were isolated from soil by bennett's agar and actinomycete isola-tion agar medium. The bacterla were identified as synonym of Actinomycetes. Based on the data obtained from its morphological and colony characteristics. The medium for production of anti-fungal materials was YEME (yeast extract 4 g, malt extract l0g, glucose 4 g, D.W 1ι, pH 7.0${\pm}$0.2). The culture conditions were 30$^{\circ}C$, 7 days and 200 rpm in shaking incubator. No. 13, No. 15 and No.28 strains were produced anti-fungal materials against fungal plant pathogens. Specially, The No. 28 strain showed a powerful biopesticide activity and broad spectrum effects of anti -fungal materials on Collectrichum coccodes, Botrytis cinerea, Cladosporium cucumerinum, Didymella bryoniae.

Keywords

References

  1. 강신욱, 정만재, 여익현, 김동호 Chitjnase를 생산하는 Pseudomonas vesicularis KW-15의 분리 및 특성, 한국 키틴 $\bulletin$ 키토산 연구회지 1998; 3(4): 303-312
  2. 김승현, 이영득, 하원숙, 노희명. 비포화 무기성 다공매질에서 잔류농약의 계면거동 및 지하수 오염현상 연구, 대한환경공학회지 1998; 20(11): 1545-1553
  3. 김창진, 이강현, 아끼라 시마즈, 권오성, 박동진. 토양 특성에 따른 다양한 희소방선균의 분리, 산업미생물학회지 1995; 23(1): 36-42
  4. 김창진, 산업적 유용 미생물(IV) '산업적 유용 방선균의 분리 및 분류', 생물산업 1997; 10(2): 34-44
  5. 농용미생물보존센터, http://kacc.rda.go.kr/?menu:m3&gotourl:orderlshop_cart.asp
  6. 박영일, 한영환. 녹강균 (Metarhizium anisopliae)의 최적 배양조건 및 효소활성, 한국미생물학회지 2000; 36(2): 97-102
  7. 오윤근, 김정호. 유기염소계 잔류농약이 제주도 연안 해양 환경에 미치는 영향, 한국수질보전학회지 1997; 13(3): 317-324
  8. 이강표, 김창남, 유주현, 오두환. Aeromonas salmonicida YA7-625에 의한 Chitinase의 생산 및 정제, 산업미생물학회지 1990; 18(6): 199-606
  9. 이규승. 농업 생태계에 대한 잔휴농약의 영향평가, 한국환경농학회지 1997; 16(1): 80-93
  10. 이은탁, 김상달. Chitinase를 생산하는 길항미생물 Serratiasp. 30959의 선발과 Fusarium 속에 대한 항진균성, 한국농화학회지 1999; 42(3): 181-187
  11. 이인경, 김창진, 김신덕, 유익동. Streptomyces parvullus균주가 생산하는 항 고추 역병성 항생물질, 산업미생물학회지 1990; 18(2): 142-147
  12. 이형환, 김기상. Bacillus thuringiensis을 이용한 미생물 살충제에 관한 연구, 산업미생물학회지 1983; 11(3): 223-231
  13. Chanpen W, Saranya T, Somsak P and Amaret B. Toxicity of Chitinase-Producing Bacillus thuringiensis ssp. kurstaki HD-l (G) toward Plutella xylostella, J. Inver. Pathol. 2000; 76: 270-227 https://doi.org/10.1006/jipa.2000.4976
  14. Goodfellow, M, Cross T and Lechevalier HA. Suprageneric classification of Actinomycetes. In S. T. Williams, M. E. Sharpe, and J. G. Holt (eds.), Bergey's Mannual of Systemtic Bacteriology Vol. 4. Williams and Wilkins, Baltimore 1989
  15. Gun Lee D, Shin SY, Maeng CY, Jin ZZ, Kim KL and Hahm KS. Isolation and characterization of a novel antifungal peptide from Aspergillus niger, Biochem. Biophys. Res. Commun. 1999; 263: 646-651 https://doi.org/10.1006/bbrc.1999.1428
  16. Han KH and Kim SD. Selection and identification of a antagonistic Promicromonospora sp. KH-28 producing chitinase and antifungal antibiotic, Kor. J. Appl. Microbiol. Biotechnol. 1999; 27(3): 191-196
  17. Hao JJ, Geng C, Xie W, Gong Z, Liu WY and Wang E. Isolation and characterization of viridin, a new 65 kDa antifungal protein from the mould Trichoderma viride, BioI. Chem. 1999; 380: 1243-1245 https://doi.org/10.1515/BC.1999.158
  18. Lachhab K, Tyagi RD and Valero JR. Production of Bacillus thuringiensis biopesticides using wastewater sludge as a raw material: effect of inoculum and sludge solids concentration, Process Biochemistry 2001; 37: 197-208 https://doi.org/10.1016/S0032-9592(01)00198-4
  19. Lee MG and Lee SR. Assessment of oncogenicity from pesticide residues in Korean food, Kor. J. Food Sci. Technol. 1995; 27(6): 871-877
  20. Marx F, Haas H, Reindl M, Stoffler G, Lottspeich F and Redl B. Cloning, structural organization and regulation of expression of the Penicillium chrysogenum paf gene encoding an abundantly secreted protein with antifungal activity, Gene 1995; 167: 167-171 https://doi.org/10.1016/0378-1119(95)00701-6
  21. Mathivanan N, Kabilan V and Murugesan K. Purification, characterization, and antifungal activity of chitinase from Fusarium chlamydosporum, a mycoparasite to groundut rust, Puccinia arachidis, Can. J. Microbiol. 1998; 44: 646-651 https://doi.org/10.1139/cjm-44-7-646
  22. Paola L, Francesca V, Antonio E, Silvia L, Aldo C and Marco G. Purification and Characterization of novel Antifungal Compounds from the sourdough Lactobacillus platarum Strain 21B, Appl. & Environ. Microbiol. 2000; 66(9): 4084-4090 https://doi.org/10.1128/AEM.66.9.4084-4090.2000
  23. Shim TH, Lee TJ, Kim KC, Ryu MJ, Jung EH and Lee HK. Survey on the contents of resodual pesticide in the agricultural products on Kangweon-Do, Kor. J. Food Hygiene 1992; 7(4): 149-156
  24. Siegel M and Sisler HD. Antifungal compounds, interactions in Ecological System, Marcel Dekker, Inc., New York 1977; 2: 277
  25. Sin WC, Lee DS, Kim TH, Woo JH, Lee JM, Kim JG and Hong SD. Isolation and Characterization of Acinetobacter sp. WC-17 Producing Chitinase, J. Microbiol. & Biotech. 1995; 5(2): 80-86
  26. Sivan A and Chet I. Degradation of fungal cell wall by lytic enzymes of Trichoderma harzianum, J. Gen. Microbiol. 1989; 135: 675-682