Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Regulation of Acetylcholine Esterase and Monoamine Oxidase in Oryzias Latipes by Carbofuran

카보푸란에 의한 송사리 acetylcholine esterase 및 monoamine oxidase의 활성조절

  • Kim, Sung-Hee (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Kim, Woo-Keun (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Lee, Jeong-Soon (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Koh, Sung-Cheol (Division of Civil and Environmental System Engineering, Korea Maritime University) ;
  • Lee, Sung-Kyu (Environmental Toxicology Laboratory, Toxicology Research Center, Korea Research Institute of Chemical Technology) ;
  • Chon, Tae-Soo (Department of Biology, Pusan National University) ;
  • Kim, Jong-Sang (Division of Civil and Environmental System Engineering, Korea Maritime University)
  • Published : 2003.03.01
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Abstract

카바메이트계 농약인 카보푸란은 어류에 대한 독성이 매우 높으며, 낮은 농도에서 어류의 척추기형이나 행동이상을 유발한다. 이러한 카보푸란의 독성기전을 밝히기 위한 일환으로 신경물질대사와 관련이 깊은 acetylcholine esterase(AChE)와 monoamine oxidase(MAO)에 미치는 농약의 효과를 송사리(Oryzias latipes; Medaka fish)를 이용하여 평가하였다. Medaka fish에 대한 카보푸란의 반수치사농도(LC$_{50}$)는 2.5 ppm이었으며, 1 ppb 카보푸란에 24시간 노출된 경우, AChE 효소활성이 머리와 몸통부위에서 각각 30, 20%씩 감소되었다. 한편, MAO 효소활성은 카보푸란의 농도가 증가함에 따라 머리부위에서는 감소한 반면, 몸통부위에서는 증가하는 경향을 보였다. 특히 카보푸란의 농도가 1 ppb에서도 송사리의 MAO 효소활성이 영향을 받는 것으로 나타나, 카보푸란에 의한 송사리의 행동이상은 AChE 활성 뿐 아니라 MAO활성의 변화에 의한 복합적인 효과일 가능성이 높다.

Keywords

References

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