Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Antibacterial Activity of Onion Pathogens and Isolation of Bacillus ehimensis YJ-4 from the Rhizosphere of Healthy Onion Roots

건전 양파 근권으로부터 Bacillus ehimensis YJ-4의 분리 및 양파 병원균들에 대한 길항력 조사

  • Published : 2003.06.01
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Abstract

This study was carried out to isolate of antagonistic bacterium to Allium cepa L. pathogens. A total of 250 strains were isolated from A. cepa L. roots. The isolates were screened for antagonism to A. cepa L. pathogens and the isolated strain No. YJ-4 was selected among these bacteria. It was identified as Bacillus ehimensis based on morphological and physiological characteristics according to the Bergey's mannual of systematic bacteriology, Sherlock system of Microbial ID Int and 165 rDNA sequences methods. Bacillus ehimensis YJ-4 showed broad spectrum of antibacterial and antifungal activities against plant pathogens as Alternaria porri, Botrytis cinerea, Erwinia carotovora subsp. carotovora Fusarium of oxysporium, penicillium sp., Pseudomonas sp., Rhizoctonia solani, Sclerotium cepivotum, Septoria sp., Stemphylium botryosum. Speially B. ehimensis YJ-4 showed high antifungal activity on growth against F. oxysporium, the causal agent of onion Fusarium wilt.

양파 근권미생물은 양파 뿌리를 균원시료로 하여 약 250여종의 근권미생물을 순수 분리하였고 이들 중에서 길항미생물은 무름병균인 Erwinia carotovora subsp. carotovora와 시들음병균인 Fuarium oxysporium에 각각 서로 대치 배양하여 두 병원균 모두에 가장 강한 길항 효과를 나타내는 YJ-4 균주를 최종 선발하였다. 선발균주 YJ-4는 Bergey's mannual of systematic bacteriology 방법에 따라 조사하여 Bacillus 속으로 판정하였고, 세포벽 지방산을 분석하여 동정하는 Sherlock system에 의해 Bacillus ehimensis와 0.778의 유의성을 확인하였으며, 165 rDNA의 부분 염기서열(609bp)로 Bacillus ehimensis와 98.36%의 높은 유사도를 보여 길항균 YJ-4는 Bacillus ehimensis로 동정하였다. B. ehimensis YJ-4로 명명한길항균은 세균인 무름병균 (Erwinia carotovora subsp. carotovora)과 세균썩음병균 (Pseudomonas sp.)에도 길항력을 나타내었고, 진균인 잘록병균 (Rhizoctonia solani), 잎마름병균 (Stemphylium botryosum), 검은무의병균 (Alternaria porri), 검은점잎마름병균 (Septoria sp.), 잿빛곰팡이병균 (Botrytis cinerea), 흑색썩음균핵병균 (Sclerotium cepivotum), 시들음병균 (Fusarium oxysporium), 푸른곰팡이병균 (Penicillium sp.)등에도 길항 효과를 나타내었다. 특히 시들음병균인 F. oxysporium에 생육억제환의 길이가 24.8mm로 가장 높은 길항력을 나타내었다.

Keywords

References

  1. Chung, H.D. (1982) Control of onion bulb rot during storage at low temperature by postharvest treatment of fungicides. J. Kor. Soc. Hort. Sci. 23, 17-22
  2. David, M.W. (1988) Biological control of soilborne plant pathogens in the rhizosphere with bacteria. Annu. Rev. Phytopathol. 26, 379-407 https://doi.org/10.1146/annurev.py.26.090188.002115
  3. Cooksey, D.A. and Moore, L.W. (1982) High frequency spontaneous mutations to agrocin 84 resistance in Agrobacterium tumefecience and A. rhizogenes. Physiol. Plant Path. 20, 129-136 https://doi.org/10.1016/0048-4059(82)90078-9
  4. Aldrich, J. and Baker, R. (1979) Biological contiol of Fusarium roseum f. sp. dianthi by Bacillus subtilis. Plant Disease Reptr. 54, 446-452
  5. Chang, I. P. and Kommedahl, T. (1968) Biological control of seedling blight of corn by coating kernels with antagonistic microorganisms. Phytopathol. 58, 1395-1401
  6. Wood, R.K.S. and Tveit, M. (1955) Control of plant diseases by use of antagonistic organisms. Bot. Rev. 21, 441-447 https://doi.org/10.1007/BF02872441
  7. Howell, C.R. (1982) Effect of Gliocladium virens on Pythium ultimum, Rhizoctonia solani, and damping-off of cotton seedlings. Phytopathol. 72, 496-502 https://doi.org/10.1094/Phyto-72-496
  8. Tu, J.G. (1980) Gliocladium virens, a destructive mycoparasite of Sclerotinia sclerotiorum. Phytopathol. 70, 670-676 https://doi.org/10.1094/Phyto-70-670
  9. Kommedahl, T. and Windels, C.E. (1978) Evaluation of biological seed treatment for controlling root disease of pea. Phytopathol. 68, 1078-1084
  10. Wildels, C.E. (1978) Growth of Penicillium oxalicum as a seed protectant aganist seedling blight of pea. Phytopathol. 68, 1656-1662 https://doi.org/10.1094/Phyto-68-1656
  11. Sivasithamparam, K., Paker, C.A. and Edwards, C.S. (1979) Bacterial antagonistics to the take-all fungus and fluorescent pseudomonads in rhizosphere of wheat. Soil Biol. Biochem. 11, 161-167 https://doi.org/10.1016/0038-0717(79)90095-6
  12. Simmiley, R.W. (1978) Colonization of wheat roots with Gaumanomyces graminis inhibited by specific soils, microorganisms and ammonium nitrogen. Soil Bio. Biochem. 10, 175-181 https://doi.org/10.1016/0038-0717(78)90093-7
  13. Kloepper, J.W. (1991) Plant growth promoting rhizobacteria as biological control agents of soil-borne disease. p142-152. In The biological control of plant disease (Bay-Peterson, J.ed.) Food and Fertilizer Technology Center, Taiwan
  14. Tang, W.H. (1994) Yield-increasing bacteria(YIB) and biocontrol of sheath blight of rice. In Improving plant productivity with rhizosphere bacteria (Ryder, M. H., Stephens, P.M. and Bowen, G.D. ed.) Commonwealth Scientific and Industrial Research Organization, Adelaide, Australia. 267-278
  15. Lee, Y.H., Shin, G.Y., Lee, E.J. and Mew, T.W. (1990). Evaluation of biocontrol activity of fluorescent pseudomonads against some rice fungal disease in vivo and greenhouse. Kor. J. Plant Pathol. 6, 73-79
  16. Kloepper, J.W., Schroth, M.N. and Miller, T.D. (1980) Effect of rhizosphere colonization by plant growth promoting rhizobacteria on potato plant development and yield. Phytopathol. 70, 1078-1082 https://doi.org/10.1094/Phyto-70-1078
  17. Suslow, T.V. and Schroth, M.N. (1982) Rhizoctonia of sugar beets: Effect of seed application and root colonization on yield. Phytopathol. 72, 199-206 https://doi.org/10.1094/Phyto-72-199
  18. Dandurand, L.M. and Knudsen, G.R. (1993) Influence of Pseudomonas fluorescens on hyphal growth and biocontrol activity of Trichoderma harzianum in the spermosphere and rhizosphere of pea. Phytopathol. 83, 265-270 https://doi.org/10.1094/Phyto-83-265
  19. Shivanna, M. B., Meera, M. S. and Hyakumachi, M. (1994) Sterile fungi from zoysia grass rhizosphere as plant growth promoters in spring wheat. Can. J. Microbiol. 40, 637-644 https://doi.org/10.1139/m94-101
  20. Jee, H. J., Nam, C. G. and Kim, C. H. (1988) Studies on biological control of phytophthora blight of red-pepper. I. Isolation of antagonists and evaluation of antagonistic activity in vivo and in greenhouse. Kor. J. Plant Pathol. 4, 305-311
  21. Lee, E. J., Jee, H. J., Park, K. S. and Kim, C. H. (1990) Studies on biological control of phytophthora blight of red-pepper. IV. Performance agents in field under polyethylene filmhouse. Kor. J. Plant Pathol. 6, 58-64
  22. Nam, C, G., Jee, H. J. and Kim, C. H. (1990) Studies on biological control of phytophthora blight of red-pepper, II. Enhancement of antagonistic activity by soil amendment with organic materials. Kor. J. Plant Pathol. 4, 313-319
  23. Yi, Y. K., Kim, J. H. and Park, W. M. (1986) Inhibition effect of avirulent Pseudomonas solanacearum on the multiplication of vilulent isolate in tobacco plant. Kor. J. Plant Pathol. 2, 114-120
  24. Joo, G. J., Kim, J. H. and Kang, S. J. (2002) Isolation and antifungal activity of Bacillis ehimensis strain YJ-37 as antagonistic against vegetables damping-off fungi. Kor. J. Life Sci. 12, 242-249 https://doi.org/10.5352/JLS.2002.12.3.242
  25. Sneath, P. A., Mair, N. S., Sharpe, M. E. and Holt, G. J. (1986) Bergey's manual of systematic bacteriology. vol. 2., The Williams & Wilkins, Baltimore.
  26. Loffler, F. E., Sun, Q., Li, J. and Tiedje, J. (2000) 16S rRNA gene-based detection of tetrachoroethene-dechlorinating desulfuromonase and dehaloccoides species. Appl. Environ. Microbiol. 66, 1369-1374 https://doi.org/10.1128/AEM.66.4.1369-1374.2000
  27. Kuroshima, K., Sakane, T., Takata, R. and Yokota, A. (1996) Bacillus ehimensis sp. nov. and Bacillus chitinolyticus sp. nov., new chitinolytic members of the genus Bacillus. Intl. J. System. Bacteriol. 46, 76-80 https://doi.org/10.1099/00207713-46-1-76
  28. Akiyama, K., Fujita, T. Kuroshima, K. Sakane, T., Yokota, A. and Takata, R. (1999) Purification and gene cloning of a chitosanase from Bacillus ehimensis EAG1. J.Biosci. Bioengin. 87, 383-385 https://doi.org/10.1016/S1389-1723(99)80050-4
  29. Shimosaka, M., Fukumori, Y., Zhang, X. Y., He, N. J., Kodaira, R. and Okazaki, M. (2000) Molecular cloning and characterization of a chitosanase from the chitosanolytic bacterium Burkholderia gladioli strain CHB101. Appl. Microbiol. Biotechnol. 54, 354-360 https://doi.org/10.1007/s002530000388