한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제20권3호
  • /
  • Pages.294-301
  • /
  • 2003
  • /
  • 1598-298X(pISSN)
  • /
  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

개에서 전층피부 창상에 대한 Hydrocolloid(Duoderm$\circledR$)과 Hydrogel(Nu-Gel$\circledR$)의 치료 효과 비교 연구

A Comparative Study of Hydrocolloid(Duoderm$\circledR$) and Hydrogel(Nu-Gel$\circledR$) Occlusive Dressing Materials in the Treatment of Full-Thickness Skin Wound in Dogs

  • Kwon, Young-Sam (College of Veterinary Medicine Kyungpook National University) ;
  • Rhee, Jung-Woo (College of Veterinary Medicine Kyungpook National University) ;
  • Jang, Kwang-ho (College of Veterinary Medicine Kyungpook National University)
  • 발행 : 2003.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

개의 전층피부 창상에서 삼출물의 정도와 창상수축, 상피화, 그리고 치유에 대해 hydrocollodi(Duoderm/$\circledR$, HC)과 hydrogel(Nu-Gel$\circledR$, HG) occlusive dressing materials 의 효과를 비교하였다. 3쌍의 정사각형 전층피부 창상을 2${\times}$2cm 의 크기로 12마리 개의 몸통 등외측 면에(6개의 창상/한 마리) 만들었다. HC와 HG, 생리 식염수(대조창)를 무작위로 배분한 2개의 창상에 각각 적용하였다. 창상의 육안적 소견과 병리조직학적 소견을 4주 동안 평가하였다. 창상유발 7일 후, 비교 그룹 간의 평균 창상수축 정도, 평균 상피화율, 평균 창상 치유율에 유의성있는 변화가 관찰되지 않았다. 창상 유발 14일(P<0.01)과 21일(P<0.05) 후, HG 처치창의 평균 상피화율이 HC 처치창의 평균 상피화율과 대조창의 상피화율보다 유의적으로 더 높게 나타났다. 창상 유발 21일 후(P<0.05), HG 처치창의 창상 수축율이 HC 처치창의 평균 창상 수축율과 대조창의 평균 창상 수축율보다 유의적으로 더 높게 나타났다. 창상 유발 21일 후(P<0.02), HG 처치창의 창상 치유율이 HC 처치창의 평균 창상 치유율과 대조창의 평균 창상 치유율보다 유의적으로 더 높게 나타났다. 창상 유발 1, 4, 7일 후, HC 처치창과 대조창의 피하에 다형핵 백혈구의 심한 침착이 관찰되었고, HG 처치창의 피하에 중등도의 다형핵 백혈구의 침착이 관찰되었으나, HG 처치창, HC 처치창 간의 어떠한 유의적인 변화도 발견되지 않았다. 창상 유발 14일 후, HG 처치창에서 상피성 세포가 유의적으로 표면 위로 자라났고, 부종은 창상 밑 조직까지 감소하였으며, 육아조직은 교원질 섬유로 대체되었다. 창상 유발 21일 후, 다른 창상에 비해, HG 처치창은 유의적으로 창상 표면이 거의 재생성 상피로 덮였으며, 육아조직은 창상 유발 14일 후와 비교해서 유의적으로 교원질 섬유로 대체되었다. 위의 모든 결과에서 보듯이, 개에서 전층피부 창상의 처치 시 HG의 사용은 HC와 생리 식염수에 비해 창상치유 복구기의 치유 속도를 촉진하는 것으로 사료된다.

This study was performed to compare the effects of hydrocolloid(Duoderm$\circledR$, HC in this study) and hydrogel (Nu-Gel$\circledR$, HG in this study) occlusive dressing materials on degree of exudate, wound contraction, epithelialization, and healing of full-thickness skin wound in dogs. Three wounds measuring 2${\times}$2 cm in size were created bilaterally(6 wounds/dog) on the dorsolateral aspect of the trunk of 12 dogs. In each dog, the wounds were treated with HC, HG, and normal saline, respectively. For a 4 week period, the wounds were evaluated gross aspects and histopathological aspects. There were no statistically significant differences between treatment groups in percentage of wound contraction, percentage of epithelialization, and percentage of wound total healing during the first week. Significant differences were first detected on day 14. On day l4(P < 0.01) and 21 (P < 0.05), mean percentage of epithelialization of HG-treated wound was significantly greater than those in HC- and normal saline-treated wound. Mean percentage of wound contraction of HG-treated wound was significantly greater than that in HC- and control wounds on day 21(P< 0.05). On day 21, mean percentage of wound healing of HG-treated wound was significantly greater than that in HC- and control wounds(P < 0.02). On day 1, 4, and 7 after wound creation, although severe infiltration of PMN (polymorphonuclear leukocyte) cells in HC- and control wounds were observed in the subcutis and moderate infiltration of PMN cells in HG-treated wound were observed in the subcutis, we did not detect significant differences. On day 14 after wounding creation, in the wounds treated with HG dressing, epithelial cells were found over the surface, and edema further decreased in the tissue under the wounds, and the granulation tissue was replaced with collagen fibers. On day 21 after wound creation, in HG-treated wound compared with other experimental material-treated wounds, regenerated epidermis covered most of the wound surface, and the granulation tissue was more replaced with collagen fibers than that on day 14. Overall results indicated that the use of hydrogel dressing materials(Nu-Gel$\circledR$) as hydrocolloid dressing (Duoderm$\circledR$) materials and normal saline treatment on full-thickness skin wounds in dogs increased the rate of healing at repair stage.

키워드

참고문헌

  1. Cellular and Molecular Immunology(2nd ed.) Functional anatomy of local and systemic immune responses Abbas,A.K.;Lichtman,A.H.;Pover,J.S.
  2. J. Surg Res v.35 The effect of occlusive dressings on collagen synthesis and re-epithelialization in superficial wounds Alvarez,O.M.;Mertz,P.M.;Eaglstein,W.H. https://doi.org/10.1016/0022-4804(83)90136-1
  3. Infect Surg v.3 Healing wounds: occlusion or exposure Alvarez,O.M.;Hefton,J.M.;Eaglastein,W.H.
  4. J. Dermatol Surg v.8 The debatable benefit of occlusive dressing for wounds Bennett,R.G. https://doi.org/10.1111/j.1524-4725.1982.tb00257.x
  5. Burns v.8 Laboratory investigation of the composition and properties of pig skin wound exudate under Op-site Buchan,I.A.;Andrews,J.K.;Lang,S.M. https://doi.org/10.1016/0305-4179(81)90088-7
  6. Handbook of inflammation(3rd ed.) Tissue repair and regeneration Castor,C.W.
  7. J. Dermatol Sci v.13 Healing of full-thickness wounds in pigs: effects of occlusive and non-occlusive dressings associated with a gel vehicle De Coninck A.;Draye,J.P.;Van Strubarq A.;Vanpee,E.;Kaufman,L.;Delaey,B.;Verbeken,G.;Roseeuw,D. https://doi.org/10.1016/S0923-1811(96)00536-1
  8. J. Invest Dermatol v.99 Comparison of the effects of moist and dry conditions on the process of angiogenesis during dermal repair Dyson,M.;Young,S.R.;Hart,J.;Lynch,J.A.;Lang,S. https://doi.org/10.1111/1523-1747.ep12614460
  9. Clin Dermatol v.2 Effect of occlusive dressings on wound healing Eaglstein,W.H.
  10. J. Am Acad Dermatol v.12 Experiences with biosynthetic dressings Eaglstein,W.H. https://doi.org/10.1016/S0190-9622(85)80006-2
  11. Arch Dermatol v.124 Optimal use of an occlusive dressing to enhance healing : effect of a delayed application and early removal on wound healing Eaglstein,W.H.;davis,S.C.;Mehle,A.L.;Mertz,P.M. https://doi.org/10.1001/archderm.124.3.392
  12. J Invest Derm v.71 New method for assessing epidermal wound healing: The effects or triamcinolone acetonide and polyethylene film occlusion Eaglstein,W.H.;Mertz,P.M. https://doi.org/10.1111/1523-1747.ep12556814
  13. Am Fam Pract v.35 Occlusive dressings Eaglstein,W.H.;Mertz,P.M.;Falanga,V.
  14. Arch Dermatol v.124 Occlusive wound dressing Falanga,V. https://doi.org/10.1001/archderm.124.6.872
  15. Science v.245 Human KGF is FGF-related with properties of a paracrine effector of epithelial cell growth Finch,P.W.;Rubin,J.S.;MIKI,T.;Ron,D.;Aaronson,S.A. https://doi.org/10.1126/science.2475908
  16. Surgical Complications and Wound Healing in the Small Animal Practice Principles of wound healing Fowler,D.
  17. J. Dermatol Surg Oncol v.8 The effect of two new dressings on epidermal wound healing Geronimus,R.G.;Robin,P. https://doi.org/10.1111/j.1524-4725.1982.tb03992.x
  18. Can Med J. v.118 Human amniotic membrane: a versatile wound dressing Gruss,J.S.;Jirsch,D.W.
  19. Arch Dermatol v.124 Facilitated wound healing using transparent film dressing following Mohs micrographic surgery Hien,N.T.;Prawer,S.E.;Katz,H.I. https://doi.org/10.1001/archderm.124.6.903
  20. Nature v.200 Effect of air exposure and occlusion on experimental human skin wounds Hinman,C.D.;Maibach,H.;Winter,G.D. https://doi.org/10.1038/200377a0
  21. J. Plast Surg v.28 The use of Op-site, a vapor permeable dressing, on skin graft donor sites Jamea,J.H.;Watson,A.C.H.
  22. J. Invest Dermatol v.94 Accelerated epithelization under a highly vapor-permeable wound dressing is associated with increased precipitation of fibrin(ogen) and fibronectin Jonkman,M.F.;Hoeksma,E.A.;Nieuwenhuis,P. https://doi.org/10.1111/1523-1747.ep12874624
  23. J. Am Vet Med Assoc v.190 Effects of nonadherent dressing materials on the healing of open wounds in dogs Lee,A.H.;Swaim,S.F.';McGuire,J.A.;Hughes,K.S.
  24. Am J. Vet. Res. v.45 Effects of gentamicin solution and cream on the healing of open wounds Lee,A.H.;Swaim,S.F.;Yang,S.T.;Wilken,L.O.
  25. The Surgical Wound The effects of dressings in wound inflammation and scar tissue Linsky,L.B.;Vovee,D.T.;Dow,T.
  26. Nature v.200 The effect of a semiocclusive dressing on the microbial population in superficial wounds Mertz,P.M.;Eaglstein,W.H.
  27. J. Am Acad Dermatol v.12 Occlusive wound dressings to prevent bacterial invasion and wound infection Mertz,P.M.;Marshall,D.A.;Eaglstein,W.H. https://doi.org/10.1016/S0190-9622(85)70091-6
  28. Vet Surg v.23 The effect of occlusive and semi-occlusive dressings on the healing of acute full-thickness skin wounds on the forelimbs of dogs Morgan,P.W.;Binnington,A.G.;Miller,C.W.;Smith,D.A.;Valliant,A. https://doi.org/10.1111/j.1532-950X.1994.tb00511.x
  29. Scand J. Plast Reconstr Surg Hand Surg v.28 Evaluation of cytokines in donor site wound fluids Ono,I.;Gunji,H.;Suda,K.;Iwatsuki,K.;Kaneko,F. https://doi.org/10.3109/02844319409022010
  30. J. Dermatol Sci v.10 Studies on cytokines related to wound healing in donor site wound fluid Ono,I.;Gunji,H.;Zhang,J.Z.;Maruyama,K.;Kaneko,F. https://doi.org/10.1016/0923-1811(95)00454-Z
  31. Biologic Basis of Wound Healing The skin in wound healing Osment,L.S.
  32. Ann Plast Surg v.4 Biological dressings in burn-a review Peter,W.J. https://doi.org/10.1097/00000637-198002000-00010
  33. Scand J. Plast Reconstr Surg v.23 The effects of occlusive dressings on inflammation and granulation tissue formation in excised wounds in rats Reuterving,C.O.;Agren,M.S.;Soderberg,T.A.;Tengrup,I.;Hallmans,G. https://doi.org/10.3109/02844318909004499
  34. Plast Reconstr Surg v.20 Bandages and topical agents Swaim,S.F.
  35. Semin Vet Med Surg(Small Anim) v.4 The effects of dressings and bandages on wound healing Swaim,S.F.
  36. Am J. Vet. Res. v.56 Effects of three occlusive dressing materials on healing of full-thickness skin wounds in dogs Swaim,S.F.;Pope,E.R.;Wagner-Mann,C.;Berg,J.N.;Ramsey.D.T.
  37. Small Animal Wound Management(2nd ed.) Wound dressing materials and topical medication Swaim,S.F.;Henderson,R.A.Jr.
  38. Small Animal Wound Management(2nd ed.) Wound dressing materials and topical medication Swaim,S.F.;Henderson,R.A.Jr.
  39. Small Animal Wound Management(2nd ed.) Wound dressing materials and topical medication Swaim,S.F.;Henderson,R.A.Jr.
  40. Compend Contin Educ v.7 The physics, physiology, and chemistry of bandaging open wounds Swaim,S.F.;Wilhalf,D.
  41. Dermato Clin v.5 The newer surgical dressings and wound healing Wheeland,R.G.
  42. J. Comp Pathol v.85 The epithelial reaction in the healing of excised cutaneous wounds in the dog Winstanley,E.W. https://doi.org/10.1016/0021-9975(75)90085-7
  43. Epidermal Wound Healing Epidermal regeneration studied in the domestic pig Winter,G.D.
  44. Nature v.193 Formation of a scab and the rate of epithelialization of superficial wounds in the skin of domestic pig Winter,G.D. https://doi.org/10.1038/193293a0
  45. Wound dressings, Wound Healing in Biochemical and Clinical Aspects Wiseman,D.M.;Pharm,M.R.;Rovee,D.T.