Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Adaptive Estimation of Hairy Root Mass Using Conductometry

  • Kim, Ji-Hyeon (Department of Chemical and Biochemical Engineering, Dongguk University) ;
  • Yoo, Young-Je (School of Chemical Engineering, Seoul National University)
  • Published : 2003.10.01
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Abstract

An accurate and efficient method for measuring the mass of hairy roots using conductometry is established. A conductivity equation expressed in terms of the concentration of the ion species in the medium is suggested. By using this equation, the effect of the individual ions on the total conductivity can be quantitatively analyzed. An equation for the in situ estimation of the cell growth coefficient for determining the mass of hairy roots is established based on measurements of the nitrogen concentration and conductivity during cultivation. The proposed equation does not require preliminary experiments to determine the cell growth coefficient. Instead, the physiological characteristics of the plant species are reflected by introducing the cellular nitrogen content. Since the cell growth coefficient is determined by measuring the major ionic nutrient concentrations, it is more effective to express the dynamics of an actual culture system. This improved method for determining the mass of hairy roots was successfully utilized in a fed-batch culture system.

Keywords

References

  1. Plant Cell Physiol. v.29 Uptake and utilization of sugars in cultured rice cell Amino,S.I.;M.Tazawa
  2. J. Microbiol. Biotechnol. v.11 Control of glucose concentration in a fedbatch cultivation of Scutellaria baicalensis G. Plant cells using a self-organizing fuzzy logic controller Choi,J.W.;J.M.Cho;Y.K.Kim;S.Y.Park;I.H.Kim;Y.H.Park
  3. Methods in plant biochemistry v.3 Enzymes of sugar metabolism Copeland,L.;P.J.Lea(ed.) https://doi.org/10.1016/B978-0-12-461013-2.50011-3
  4. Plant Cell Culture A Practical Approach Dixon,R.A.
  5. Plant Tissue Culture and Its Biotchnological Application Current problems in regulation of nitrogen metabolism in plant cell cultures Dougall,D.K.;W.Barz(ed.);E.Reinhard(ed.);M.H.Zenk(ed.)
  6. Proceedings of Annual Meeting of AIChE Culture of Nicotiana tabacum with on-line product removal Gomez,P.L.;A.E.Humphrey
  7. Planta v.124 Further studies on the relationship between the rates of nitrate uptake, growth and conductivity changes in the medium of plant cell cultures Hahlbrock,K. https://doi.org/10.1007/BF00388694
  8. J. Microbiol. Biotechnol. v.11 Development of high performance liquid chromatography for paclitaxel purification from plant cell cultures Kim,J.H.;H.K.Choi;S.S.Hong;H.S.Lee
  9. J. Microbiol. Biotechnol. v.12 Optimization of SOD biosynthesis by controlling sucrose concentration in the culture of carrot hairy root Kim,J.H.;Y.J.Yoo
  10. J. Chem. Eng. Japan v.24 Conductometric estimation of main inorganic nutrients in plant cell culture Kino-Oka,M.M.;M.Taya;S.Tone https://doi.org/10.1252/jcej.24.381
  11. Anal Chem. v.31 Use of Dinitrosalicylic acid reagent for determination of reducing sugar Miller,G.L. https://doi.org/10.1021/ac60147a030
  12. Biotechnol. Letters v.8 Nicotine production by hairy root cultures of Nicotiana rustica: Fermentation and product recovery Rhodes,M.J.C.;M.Hilton;A.J.Parr;R.J.Robins https://doi.org/10.1007/BF01026743
  13. Can. J. Botany v.52 Parameter for growth measurement in suspension cultures of plant cell Rose,D.;S.M.Martin https://doi.org/10.1139/b74-114
  14. Biotechnol. Bioeng. v.35 Determination of growth rate for plant cell cultures: Comparative studies Ryu,D.D.Y.;S.O.Lee;R.J.Romani https://doi.org/10.1002/bit.260350312
  15. Biotechnol. Bioeng. v.26 Characterization of intracellular accumulation of Poly-β-Hydroxybutyrate(PHB) in individual cells of Alcaligenes eutrophos H16 by Flow Cytometry Srienc,F.;B.Arnold;J.E.Bailey https://doi.org/10.1002/bit.260260824
  16. J. Ferment. Bioeng. v.73 Turbidimetric measurement of cell biomass of plant cell suspensions Tanaka,H.;H.Aoyagi;T.Jitsufuchi https://doi.org/10.1016/0922-338X(92)90527-2
  17. Standard methods of chemical analysis(6th ed.) v.2 Industrial and Products and Noninstrumental Methods Part B Welcher,F.J.
  18. Biotechnol. Techniques v.5 Measurement of Myxobacterial growth using conductivity Yasouri,F.N.;H.A.Foster