International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Comparative Study on Trichoplusia ni Tn 5B1-4 Cells and Bombyx mori BmN Cells for Recombinant Endostatin Production

  • Sohn, Bong-Hee (Department of Sericultue and Entomology, The National Institute of Agricultural Science and Technology) ;
  • Lee, Jong-Min (Department of Genetic Engineering and Plant Metabolism Research Center, Kyung Hee University) ;
  • Kang, Pil-Don (Department of Sericultue and Entomology, The National Institute of Agricultural Science and Technology) ;
  • Lee, Sang-Uk (Department of Sericultue and Entomology, The National Institute of Agricultural Science and Technology) ;
  • Kim, Yong-Soon (Department of Sericultue and Entomology, The National Institute of Agricultural Science and Technology) ;
  • Chung, In-Sik (Department of Genetic Engineering and Plant Metabolism Research Center, Kyung Hee University)
  • Published : 2003.12.01
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Abstract

The recombinant plasmids harboring a heterologous gene coding mouse endostatin were transfected and expressed stably in Trichoplusia ni Tn 5B1-4 cells and Bombyx mori BmN cells, respectively. Recombinant endostatin expressed in the stably transformed Tn 5B1-4 and BmN cells was secreted into the medium. BmN cells are relatively lower in maximum cell growth and recombinant endostatin production than Tn 5B 1-4 cells. Recombinant endostatin was also purified to homogeneity using a simple one-step ${Ni^2+}$ affinity fractionation method. Purified recombinant endostatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition $({ED_50})$ for recombinant endostatin was approximately 0.35 ${\mu}g$/ml.

Keywords

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