The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Liquid Chromatographic Determination of Astaxanthin in Phaffia rhodozyma

HPLC에 의한 Phaffia rhodozyma 중의 Astaxanthin의 측정법

  • Xu, Bao-Jun (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam Natioanl University) ;
  • Fang, Zhe-Ming (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam Natioanl University) ;
  • Kang, Hyo-Jin (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam Natioanl University) ;
  • Wang, Qi-Jun (College of Food and Bioengineering, South China University of Technology) ;
  • Mo, Eun-Kyoung (Biotechnology Research Institute) ;
  • Sung, Chang-Keun (Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam Natioanl University)
  • Published : 2003.09.01
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Abstract

A sensitive and accurate method for the detection of astaxanthin was developed. The optimum HPLC system for the extraction and quantification of astaxanthin was based on a reversed phase column, and a quaternate mobile phase contained dichloromethane. The amount of astaxanthin can be accurately quantified in Phaffia rhodozyma cultures. The results of this study suggest that the mixture solution of dimethylsulphoxide and acetone is the optimal extraction solvent for astaxanthin and samples treatment for astaxanthin analysis must control low temperature and absent light operation strictly.

Astaxanthin은 수산양식에 필수적인 carotenoid 첨가물일 뿐 아니라 우수한 항암 및 항산화 물질로서 알려져 있다. 현재 astaxanthin은 P. rhodozyma에 의해 생산 할 수 있으나 P. rhodozyma에 함유된 유효성분인 astafanthin의 정량적인 분석 방법이 제시되지 못하였다. 본 연구에서는 astaxanthin에 대한 정확한 정성법과 정량분석법을 제안하였다. 이 방법은HPLC의 역상컬럼을 사용하여 dichlorornethane및 4개의 혼합 용매를 이동상으로 사용하는 것이다. 이 제시된 방법을 활용했을 때 P. rhodozyma 배양액 중의 astaxanthin의 함유량을 정확하게 검출할 수 있다. DMSO 와 acetone의 혼합용매가 최적의 astaxanthin 추출용매이었고 또한 낮은 온도와 어두운 환경 이 최적의 시료처리조건이었다.

Keywords

References

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