Conception Rate of Ovulation-Estrus Synchronization Method in Hanwoo

한우에 있어서 발정ㆍ배란 동기화법에 의한 수태율

  • Published : 2003.09.01

Abstract

This objective of this experiment were to evaluate the effect of various estrus synchronization programs on estrus detection rate and pregnancy rate in Hanwoo. After Postpartum 60 Days, a total of 150 cows divided into 2 groups. Cows Group 1 were treated with one luteolytic dosage of PGF$_2$$\alpha$(25 mg, im; lutalyse. USA) on Day 0, and with a second dosage 14 d later; cows in Group 2 were treated with GnRH(l00 $\mu\textrm{g}$, im; Conceral. Korea) on Day 0, PGF$_2$$\alpha$ 7 d later, GnRH 2 d later, and then time-inseminated approximately 16 h after this second treatment with GnRH. Ovarian morphology was monitored cows by trans-rectal ultrasonography from 24 hr to 32 hrs after second GnRH injection. The result obtained summarized as follows: 1. Cows synchronization of estrus with GnRH+PGF$_2$$\alpha$+GnRH(Ov-synch) and PGF$_2$$\alpha$ were 91.3 and 40.0%, respectively. 2. Induced ovulation were 24 to 32hr after the second GnRH injection, but high induced ovulation was 28hr. 3. High conception rate were 24hr insemination after the second GnRH injection. 4. Conception rate with PGF$_2$$\alpha$, CIDR and GnRH treatment were 50.0, 36.0 and 76.9%, respectively.

본 연구는 한우에 PGF$_2$$\alpha$와 GnRH+PGF$_2$$\alpha$+GnRH(Ov-synch)를 처리하여 발정 및 배란을 동기화 시켰으며, 2차 GnRH 투여후 배란시간, 2차 GnRH 투여후 시간 경과에 따른 수태율과 발정ㆍ배란 동기화법에 의한 수태율을 조사하고자 실시하였다. 시험축은 총 4개 농가에서 경산우 150두를 무작위로 선발하여 시험에 공시하였으며, 발정ㆍ배란동기화 방법에 따라 발정을 유기한 후 1회 인공수정을 실시하고 수태율에 미치는 영향을 조사하였다. 호르몬처리 방법으로는 GnRH+PCF$_2$$\alpha$+GnRH(Ov-synch)와 PGF$_2$$\alpha$를 이용한 발정동기화 방법을 사용하였다. 2차 GnRH 투여 후 배란시간을 알아보기 GnRH 투여 후 24시간 후부터 32시간까지 2시간 간격으로 초음파 Sonovet-600(Medison. Korea)를 이용하여 난소를 촬영하였다. 1. 호르몬 투여후 발정동기 화율은 PGF$_2$$\alpha$ 투여구에서 40.0%와 GnRH+PGF$_2$$\alpha$+GnRH(Ov-synch)처리구에서 91.3%로 나타났다. 2. 2차 GnRH 주사후 24시간에 배란이 시작되어 32시간에 배란이 종료되었으며, 배란율은 28시간째에 46.6%가 배란되어 가장 높게 나타났다. 3. 2차 GnRH주사후 6∼24시간에 수정한 군이 6시간 이전과 30시간 이후에 수정시킨 군에 비해 높은 수태율을 나타냈다. 4. 호르몬 처리별 수태율은 PGF$_2$$\alpha$, CIDR 및 GnRH+PGF$_2$$\alpha$+GnRH(Ov-synch)에서 각각 50.0, 36.0와 76.9%로 GnRH+PGF$_2$$\alpha$+CnRH(Ov-synch)군에서 가장 높은 수태율을 나타냈다.

References

  1. Lucy, M. C., Stevenson, J. S. and Call, E. P. 1986. Controlling first service and calving interval by prostaglandin $F_2\alpha$, gonadotropin-releasing hormone, and timed insemination. J. Dairy. Sci. 69:2186-2294 https://doi.org/10.3168/jds.S0022-0302(86)80652-X
  2. Martines, N. F., Kastelic, J. P., Adams, G. P., and Mapletoft, R. J. 2001. The use of a progesterone-releasing device(ClDR-B) or melengestrol acetate with GnRH, LH, or estradiol benzoate for fixed-time Al in beef heifers. J. Anim. Sci. 80:1746-1751
  3. Pursley, J. R., Kosorok, M. and Wiltbank, M. 1990. Reproductive management of lactating dairy cows using synchronization of ovulation. J. Dairy. Sci. 80:301-306 https://doi.org/10.3168/jds.S0022-0302(97)75938-1
  4. Pursley, J. R., Mee, M. O. and Wilbank, M. C. 1995. Synchronization of ovulation in dairy cows using $PGF_2\alpha$ and GnRH. Theriogenology 44:915-923 https://doi.org/10.1016/0093-691X(95)00279-H
  5. Pursley, J. R., Wilbank, M. C., Stevenson, J. S., Ottobre, J. S., Garverick, H. A. and Anderson, L. L. 1997. Pregnancy raye per artificial insemination for cows and heifers inseminated at a synchronized ovulation or synchronized estrus. J. Dairy. Sci. 80:295-300 https://doi.org/10.3168/jds.S0022-0302(97)75937-X
  6. Pursley, J. R., Silcox, R. and Wilbank, M. C. 1998. Effect of time of artificial insemination on pregnancy rates, calving rates, pregnancy loss, and gender ratio after synchronization of ovulation in lactating dairy cows. J. Dairy. Sci. 81:2139-2144 https://doi.org/10.3168/jds.S0022-0302(98)75790-X
  7. Roche, J. F., Prendiville, D. J. and Davis, W. D. 1987. Calving rate following fixed time insemination after a 12-day progesterone treatment in dairy cows, beef cows and heifers. Vet. Rec, 101(21):417-419
  8. Ryan, D. P., Bao, M. K. and Williams, G. L. 1995. Metabolic and luteal sequelae to heightened dietary fat intake in under nourished, anestrous beef cows induced to ovulate. J. Anim. Sci. 73:2086-2093 https://doi.org/10.2527/1995.7372086x
  9. Ryan, D. P., Snijders, S., Yaakub, H. and O'Farrell, K. J. 1995. An evaluation of estrus synchronization programs in reproductive management of dairy herds. J. Anim. Sci. 73:3687-95 https://doi.org/10.2527/1995.73123687x
  10. Stephens. L. A. and Rajamahendran, R. 1996. A comparison of two estrus synchronization methods in beef heifers. Can. J. Anim. Sci. 78:437-439 https://doi.org/10.4141/A98-043
  11. Stephen, J. L., Ken, E. L., Henry, J. C., David, F. K. and Gregory, P. K. 1998. Measures of estrus detection and pregnancy in dairy cows after administration of gonadotropinreleasing hormone within an estrus synchronization program based on prostaglandin$F_2\alpha$. J. Dairy. Sci. 81:375-381 https://doi.org/10.3168/jds.S0022-0302(98)75587-0
  12. Stevenson, J. S., Lucy, M. C. and Call, E. P. 1987. Failure of time inseminations and associated luteal function in dairy cattle after two injections of prostaglandin $F_2\alpha$. Theriogenology Vol. 28 No. 6
  13. Stevenson, J. S., Kobayashi, Y. and Thompson, K. E. 1999. Reproductive performance of dairy in various programmed breeding systems including ovsynch and combinations of gonadotropin-releasing hormone and prostaglandin $F_2\alpha$. J. Dairy. Sci. 82:506-15 https://doi.org/10.3168/jds.S0022-0302(99)75261-6
  14. Stevenson, J. S., Mee, M. O. and Stewart, R. E. 1989. Conception rates and calving intervals after prostaglandin $F_2\alpha$ or prebreeding progesterone in dairy cows. J. Dairy. Sci. 72:208-18. https://doi.org/10.3168/jds.S0022-0302(89)79099-8
  15. Stevenson, J. S., Smith, J. F. and Hawkins, D. E. 2000. Reproductive outcomes for dairy heifers treated with combinations of prostaglandin $F_2\alpha$ alpha, norgestomet, and gonado-tropin-releasing hormone. Daily. Sci. 83:2008-15 https://doi.org/10.3168/jds.S0022-0302(00)75079-X
  16. Stevenson, J. S., Thompson, K. E., Forbes, W. L., Lamb, G. C., Grieger, D. M. and Corah, L. R. 2000. Synchronizing estrus and(or) ovulation in beef cows after combinations of GnRH, norgestomet, and prostaglandin $F_2\alpha$ with or without timed insemination. J. Anim. Sci. 78: 1747-1752 https://doi.org/10.2527/2000.7871747x
  17. Thatcher, W. W., Macmillan, K. L., Hansen, P. J. and Drost, M. 1989. Concepts for regulation of corpus luteum function by the conceptus and ovarian follicle to improve fertility. Theriogenology 31:149-164
  18. Twagiramungu, H., Guilbault, L. A., Proulx, J., Villeneuve, P. and Dufour, J. J. 1992. Infiuence of an agonist of gonadotropin-releasing hormone (buserelin) on estrus synchronization and fertility in beef cows. J. Anim. Sci. 70:1904-1910 https://doi.org/10.2527/1992.7061904x
  19. Xu, Z. Z., Burton, L. J., and Macmillan, K. L. 1997. Reproductive performanmce of lactating dairy cows followong estrus synchronzation regimens with $PGF_2\alpha$ and progesterone. Theriogenlogy 47:687-701 https://doi.org/10.1016/S0093-691X(97)00027-7
  20. Xu, Z. Z. and Burton, L. J. 2000. Estrus synchronization of lactating dairy cows with GnRH, progesterone, and prostaglandin $F_2\alpha$. J. Dairy. Sci. 83:471-6 https://doi.org/10.3168/jds.S0022-0302(00)74905-8
  21. Yavas, Y., Johnson, W. H. and Walton, J. S. 1999. Modification of follicular dynamics by exogenous FSH and progesterone, and the induction of evaluation using hCG in postpartum beef cows. Theriogenology 52:949-963 https://doi.org/10.1016/S0093-691X(99)00185-5