Conjugated Linoleic Acid(CLA)가 축적된 돈육의 저장기간중 이화학적 특성변화

Changes of Physicochemical Properties of Conjugated Linoleic Acid(CLA) Accumulated Pork during Storage Time

  • 이정일 (경상남도 첨단양돈연구소) ;
  • 정재두 (경상남도 첨단양돈연구소) ;
  • 이진우 (경상남도 첨단양돈연구소) ;
  • 이제룡 (경상남도 첨단양돈연구소) ;
  • 정진연 (경상대학교 축산과학부) ;
  • 양한술 (경상대학교 축산과학부) ;
  • 주선태 (경상대학교 축산과학부)
  • 발행 : 2003.09.01

초록

Conjugated linoleic acid(CLA)를 비육돈에 급여하여 비육돈체조직내에 CLA를 축적시키고, CLA가 축적된 돈육의 품질 특성에 대한 연구를 수행하였다. 대조구는 80∼110 kg 까지는 일반사료회사에서 생산하는 사료를 급여하였으며, 처리구 1은 출하(출하체중: 110 kg) 2주전부터 일반사료회사에서 생산하는 사료에 CLA를 1.25% 첨가하여 급여하였으며, 처리구2는 출하 2주전부터 사료함량에 CLA를 2.5% 첨가하여 급여하였다. 처리구 3은 사료함량에 1.25% CLA를 첨가하여 4주간 급여하였으며, 처리구 4는 2.5% CLA를 4주간 급여하였다. 급여기간이 끝난 후 일괄적으로 함양도축장에서 도축하여 돈육의 등심부위를 함기포장하여 냉장온도(4'E)에서 14일간 저장하면서 각 실험항목을 조사하였다 저장기간에 따른 돈육등심의 일반성분, 이화학적 특성 및 관능평가등을조사하여 CLA가 축적된 돈육의 저장기간에 따른 품질특성을 규명하고자 하였다. pH변화를 보면 전 저장기간동안 대조구에 비하여 CLA 급여 처리구가 유의적으로 높은 pH를 보여(p<0.05) CLA 급여가 PH 변화에 영향을 미치는 것으로 나타났다. 저장기간에 따른 pH 변화에서 모든 처리구가 저장기간이 경과함에 따라 pH가 증가하는 경향을 보였다. CLA급여 수준과 급여기간이 등심근내 조지방 함량에는 유의적인 영향을 미치는 것으로 나타났으며(p<0.05), 대조구와 CLA 급여처리구간에는 대조구에 비하여 CLA급여 처리구가 높은 조지방 함량을 보였다(p<0.05). 보수성은 대조구에 비하여 CLA 급여 처리구가 높은 경향을 보였다. 저장 초기에는 대조구에 비하여 CLA 급여 처리구가 유의적으로(p<0.05) 낮은 육즙손실을 보였으며, 모든 처리구가 저장기간이 경과함에 따라 육즙손실이 유의적으로 감소하는 경향을 보였다. CLA 급여수준과 급여기간이 육색과 총육색소 함량에는 영향을 미치지 않는 것으로 나타났다. 관능적 특성평가 중 육색과 육즙참출은 대조구와 비교하여 CLA급여 처리구가 유의적인 차이가 없었으나, 근내지방 축적 정도와 전체적인 기호성은 대조구에 비하여 CLA 급여 처리구가 유의적으로 높은 평가를 받았다(p<0.05).

To investigate the effects of dietary conjugated linoleic acid 011 changes of physicochemical properties of pork. Twenty pigs were divided into 5 treatment groups and subjected to each experimental diet(0, 1.25% and 2.5% CLA for 2 weeks, 1.25% and 2.5% CLA for 4 weeks, as total fed diet before slaughtering(about 110 kg). Pork loin samples were aerobically packed and stored for 14 days at 4$^{\circ}C$. Samples were then analyzed for general compositions, physicochemical characteristics and sensory evaluation. pH value of CLA fed group pork was significantly increased than that of control group(p<0.05). Crude fat content of CLA treated group pork was significantly higher than the control pork(p<0.05), but there were no significant differences in crude protein, crude ash and total moisture contents between control and CLA treated groups. AU CLA fed group pork showed higher WHC value than control pork. Drip loss was significantly lower compared to those of control porks. No significant differences in lean meat and fat color(CIE L*, a*, b*) were observed between control and the CLA treated group porks. Meanwhile, CLA pork tended to be lower in content of total heme pigment compared to control pork. No remarkable differences were found in sensory properties(color and drip loss) among control and CLA diet-fed group porks. Marbling score and acceptability were significantly increased in CLA diet-fed pork compared to the control pork.

키워드

참고문헌

  1. AOAC (1995) Official Methods of Analysis. Association of Offcial Analytical Chemists, Washington, DC
  2. Aydin, R., Pariza, M. W., and Cook, M. E. (2001) Olive oil prevents the adverse effects of dietary conjugated linoleic acid on chick hatchability and egg quality. J. Nutr. 131(3), 800-806
  3. Bartholmew, D. T. and Blumer, J. N. (1977) Microbial interactions in country-style hams. J. Food Sci. 42, 498 https://doi.org/10.1111/j.1365-2621.1977.tb01531.x
  4. Belury, M. A. (1995) Conjugated dienoic linoleate: a polyunsaturated fatty acid with unique chemoprotective properties. Nutr. Rev. 53, 83-89 https://doi.org/10.1111/j.1753-4887.1995.tb01525.x
  5. Bendall, J. R. and Swatland, H. J. (1988) A review of the relationships of pH with physical aspects of pork quality. Meat Sci. 24, 85-126 https://doi.org/10.1016/0309-1740(88)90052-6
  6. Cannon, J. E., Morgan, J. B., Heavner, J., McKeith, F. K., Smith, G. C., and Meeker, D. L. (1995) Pork quality audit: A review of the factors influencing pork quality. J. Muscle Foods 6, 369-402 https://doi.org/10.1111/j.1745-4573.1995.tb00581.x
  7. Chan, W. K. M., Faustman, C., and Renerre, M. (1996) Model system for studying pigment and lipid oxidation relevant to muscle based foods. In Natural antioxidants, chemistry, health effects and application. Fereidoon Shahidi(Ed.), Ch. 20, ACDS Press, Champaign, Illinois, pp. 319-330
  8. Chin, S. F., Liu, W., Storkson, J. M., Ha, Y. L., and Pahza, M. W. (1992) Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcino-gens. J. Food Composition and Analysis 5, 185-197 https://doi.org/10.1016/0889-1575(92)90037-K
  9. Chin, S. F., Storkso, J. M., Albright, K. J., Cooke, M. E., and Pariza, M. W. (1994) Conjugated Imoleic acid is a growth factor for rats as shown by enhanced weight gain and improved feed efficiency. J. Nutr. 124, 2344-2349
  10. Cook, M. E., Miller, C. C., Park, Y., and Pariza, M. W. (1993) Immune modulation by altered nutrient metabolism: nutritional control of immune induced growth depression. Poult. Sci. 72, 1301-1305 https://doi.org/10.3382/ps.0721301
  11. Deymer, D. I. and Vandekerckhove, P. (1979) Compounds determining pH in dry sausage. Meat Sci. 3, 161 https://doi.org/10.1016/0309-1740(79)90033-0
  12. Doyle, E. (1998) Scientific forum explores CLA knowledge. Inform 9, 69-73
  13. Du, M., Ahn, D. U., Nam, K. C., and Sell, J. L. (2000) Influence of dietary conjugated linoleic acid on volatile profiles color and lipid oxidation of irradiated raw chicken meat. Meat Sci. 56, 387 https://doi.org/10.1016/S0309-1740(00)00067-X
  14. Dugan, M. E. R., Aalhue, J. L., Jeremiah, I. E., Kramer, J. K. G., and Schaefer, A. L. (1999) The effects of feeding conjugated linoleic acid on subsequent pork quality. Can. J.Anim. Sci. 79, 45-51 https://doi.org/10.4141/A98-070
  15. Dunshea, F. R., Ostrowska, E., Luxford, B- Smits, R. J., Campbell, R. G., D'Souza, D. N., and Mullan, B. P. (2002) Dietary conjugated linoleic acid can decrease backfat in pigs housed under commercial conditions. Asian-Aust. J. Anim. Sci. 15(7), 1011-1017 https://doi.org/10.5713/ajas.2002.1011
  16. Eggert, J. M., Belury, M. A., Kempa-Steczko, A. Mills, S. E., and Schinckel, A. (2001) Effects of conjugated linoleic acid on the belly firmness and fatty acid composition of genetically lean pigs. J. Anim. Sci. 79(11), 2866-2872 https://doi.org/10.2527/2001.79112866x
  17. Eggert, J. M., Carroll, A. L., Richert, B. T., Gerrard, D. E., Forrest, J. C., Bowker, B. C., Wynveen, E. J., Hammelman, J. E., and Schinckel, A. P. (1999) Effects of conjugated linoleic acid (CLA) on the growth, carcass composition and pork quality of two genotypes of lean gilts. J. Anim. Sci. 77(1), 178
  18. Fitch, H. B. (1996) Conjugated linoleic acid offers research promise, Inform 7, 152-159
  19. Fogerty, A. C., Ford, G. L., and Svoronos, D. (1988) Octadeca-9, 11-dienoic acid in food stuffs and in the lipids of human blood and breast milk. Nutrition Reports International 38, 937-944
  20. Ha, Y. L., Grimm, N. K., and Pariza, M. W. (1987) Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinosenesis 8, 1881-1887 https://doi.org/10.1093/carcin/8.12.1881
  21. Ha, Y. L., Park, G. B., Kang, S. J., Shim, K. H., and Kim, J. O. (1998) Newly recognized multifuctional fatty acids for the production of high quality meat, fish and agricultural products. Report of Ministry of Agriculture and Forestry, pp. 1-314
  22. Ha, Y. L., Storkson, J., and Pariza, M. W. (1990) Inhibition of benzo[a]Pyrene induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Res. 50, 1097-1101
  23. Holly, R. A., Gariepy, C., Delaquis, P., Doyon, G., and Gagnon, J. (1994) Static contro11ed($CO_2$) atmosphere packaging retail ready pork. J. Food Sci. 59(6), 1296 https://doi.org/10.1111/j.1365-2621.1994.tb14699.x
  24. Honikel, K. O. (1987) How to measure the water-holding capacity of meat. Recommendation of standardised methods. In Evaluation and control of meat quality in pigs, Tarrent, P. V., Eikelenboom, G., and Monin, G.(eds.), Martinus Nijhoff Publishers, Dordrecht, pp. 129-142
  25. Homsey, H. C. (1956) The colour of cooked cured pork. I. Estimation of the nitric oxide-haem pigments. J. Sci. FoodAgric. 7, 534-540 https://doi.org/10.1002/jsfa.2740070804
  26. Houseknecht, K. L., Vanden Heuvel, J. P., Moya-Camarena, S. Y., Portocarrero, C. P., Peck, L. W., Nickel, K. P., and Belury, M. A. (1998) Dietary conjugated Imoleic acid normalizes impaires glucose tolerance in the Zucker diabetic fatty fa/fa rat. Biochem. Biophys. Res. Comm. 244, 678-682 https://doi.org/10.1006/bbrc.1998.8303
  27. Hughes, P. E., Hunter, W. J., and Tove, S. B. (1982) Biohydrogenation of unsaturated fatty acids, Purification and properties of cis, 9-transoctadeca-dienoate reductase. J. Biol Chem. 257, 3643-3649
  28. IP, C., Scimeca, J. A. and Thompson, H. J. (1994) Conjugated linoleic acid. A powerful anticarcinogen from animal fat sources. Cancer. 74, 1050-1054
  29. Joo, S. T., Lee, J. I., Ha, Y. L., and Park, G. B. (2002) Effects of dietary conjugated linoleic acid on fatty acid composition, lipid oxidation, color and water-holding capacity of pork loin. J. Anim. Sci. 80, 108-112 https://doi.org/10.2527/2002.801108x
  30. Larsen, S. T., Wiegand, B. R., Jr. Parris; F. C., and Sparks, J. C. (1999) Effects of CLA supplementation on ham quality characteristics of crossbred growing-finishing barrows. J. Anim. Sci. 77(1), 47
  31. Lee, J. I., Joo, S. T., Park, T. S., Shin, T. S., Ha, Y. L., and Park, G. B. (1999) Changes in physico-chemical properties of accumulated broiler by conjugated linoleic acid(CLA) during cold storage. Kor. J. Food Sci. Ani. Resour. 19(1), 88-99
  32. Lee, K. N., Kritchevsky, D., and Pariza, M. W. 1994. Conjugated linoleic acid and atherosclerosis in rabits. Atherosclerosis. 108, 19-25 https://doi.org/10.1016/0021-9150(94)90034-5
  33. McCaw, J., Ellis, M., Brewer, M. S., and McKeith, F. K. (1997) Incubation - temperature effects on physical characteristics of normal, DFD and halothane carrier pork longissimus. J. Anim. Sci. 75, 1547-1552 https://doi.org/10.2527/1997.7561547x
  34. Melo, T. S., Blumer, T. N., Swaisgood, M. E., and Monroe, R. J. (1974) Catheptic enzyme activity in aged country-style hams as influenced by precuring treatments. J. Food Sci. 39, 511 https://doi.org/10.1111/j.1365-2621.1974.tb02936.x
  35. Miller, C. C., Park, Y., Pariza, M. W., and Cook, M. E. (1994) Feeding conjugated linoleic acid to animals partially overcomes catabolic responses due to endotoxin injection. Biochem. Biophys. Res. Commun. 198, 1107-1112 https://doi.org/10.1006/bbrc.1994.1157
  36. Mossoba, M. M., McDonald, R. E., and Armstrong, D. J. (1991) Identification of minor c18 triene and conjugated diene isomers in hydrogenated soybean oil and margarine byGC-MI-FT-IR spectroscopy. J. Chmmatogr. Sci. 29, 324-330 https://doi.org/10.1093/chromsci/29.8.324
  37. Nicolosi, R. J., Courtemanche, K. V., Laitinen, L., Scimeca, J. A., and Huth, P. J. (1993) Effect of feeding diets enriched in conjugated linoleic acid on lipoproteins and aortic atherogenesis in hamsters, Circulation 88 suppl. 2458
  38. Nicolosi, R. J., Rogers, E. J., Khtchevsky, D., Scimeca, J. A., and Huth, P. J. (1997) Dietary conjugated linoleic acid reduces plasma lipoproteins and early aortic atherosclerosis in hypercholesterolemic hamsters. Artery 22, 266-277
  39. Offer, G. 1991. Modelling of the formation of pale, soft and exudative meat: Effects of chilling regime and rate and extent of glycolysis. Meat Sci. 30, 157-169 https://doi.org/10.1016/0309-1740(91)90005-B
  40. O'Quinn, P. R., Smith, I. I., Nelssen, J. L., Tokach, M. D., Goodband, R. D., and Owen, K. Q. (2000) A comparison of modified tall oil and conjugated linoleic acid on growing-finishing pig growth performance and carcass characteristics. J. Anim. Sci. 78, 2359-2368 https://doi.org/10.2527/2000.7892359x
  41. Pariza, M. W. (1997) Conjugated linoleic acid a newly recognised nutrient. Chem. Ind.(London) 12, 464-466
  42. Park, G. B., Lee, J. I., Ha, Y. L., Kang. S. J., Jin, S. K., and Joo, S. T. (1998) Effect of conjugated Jinoleic acid on Fatty acid composition and lipid oxidation of egg yolk. Kor. J. Food Sci. Ani. Resour. 18(4), 339-347
  43. Park, G. B., Lee, J. I., Park, T. S., Kim, 'J. H., Shin, T. S., Kang, S. J., Ha, Y. L., and Joo, S. T. (1999) Effects of dietary conjugated linoleic acid(CLA) on cholesterol andCLA content of egg yolks. Kor. J. Ani. Sci. 41(1) , 65-74
  44. Park, Y. H., Albright, K. J., Liu, W., Storkson, J. M., Cook, M. E., and Pariza, M. W. (1997) Effect of conjugatedlinoleic acid on body composition in mice. Lipids 32, 853-858 https://doi.org/10.1007/s11745-997-0109-x
  45. Park, Y., Albright, K. J., Storkson, J. M., Cook, M. E., and Pariza, M. W. (1999) Changes in body composition during feeding and withdrawal of dietary conjugated linoleic acid. Lipids 34, 243-248 https://doi.org/10.1007/s11745-999-0359-7
  46. SAS (1999) SAS/STAT Software for PC. Release 6.11, SAS Institute, Cary, NC, U.S.A
  47. Sutton, D. (1997) Studies on the Napole gene and pork quality. Ph. D. dissertation, Univ. of Illinois, Urbana, IL
  48. Warner, R. D., Kauffman, R. G. and Russell, R. L. (1993) Quatity attributes of major porcine muscles: A comparison with longissimus lumborum. Meat Sci. 33, 359-372 https://doi.org/10.1016/0309-1740(93)90007-5
  49. Wiegand, B. R. Jr., Parrish, F. C., and Sparks, J. C. (1999) Effects of CLA supplementation on pork quality characte-ristics in crossbred growing-fmishing barrows. J. Anim. Sci. 77(1), 47
  50. Yurawecz, M. P., Hood, J. K., Mossoba, M. M., Roach, J. A., and Ku, Y. (1995) Furan fatty acids determined as oxidation products of conjugated octadecadienoic acid. Lipids 30, 595-598 https://doi.org/10.1007/BF02536995